Polymorphs of selinexor

ABSTRACT

The present invention relates to crystalline forms of the compound represented by Structural Formula I, and compositions comprising crystalline forms of the compound represented by Structural Formula I described herein. The crystalline forms of the compound of Structural Formula I and compositions comprising the crystalline forms of the compound of Structural Formula I provided herein, in particular, single crystalline Form A, can be incorporated into pharmaceutical compositions, which can be used to treat various disorders associated with CRM1 activity, including cancer. Also described herein are methods for preparing the compound of Structural Formula I and its single crystalline forms.

RELATED APPLICATION

This application claims the benefit of U.S. Provisional Application No.62/038069 filed on Aug. 15, 2014. The entire teachings of the aboveapplication are incorporated herein by reference.

BACKGROUND OF THE INVENTION

International Publication No. WO 2013/019548 describes a series ofcompounds that are indicated to have inhibitory activity againstchromosomal region maintenance 1 (CRM1, also referred to as exportin 1or XPO1) and to be useful in the treatment of disorders associated withCRM1 activity, such as cancer.(Z)-3-(3-(3,5-bis(trifluoromethyl)phenyl)-1H-1,2,4-triazol-1-yl)-N′-(pyrazin-2-yl)acrylohydrazide(also referred to as selinexor) is one of the compounds disclosed inInternational Publication No. WO 2013/019548. Selinexor has the chemicalstructure shown in Structural Formula I:

The solid form of a compound can be important in the formulation ofpharmaceutical compositions. For example, crystalline and amorphousforms of a compound can have different physical properties (e.g.,stability, dissolution rate, density, etc.) relating to theirsuitability for use in pharmaceutical compositions. The difference inphysical properties can also affect a crystalline or amorphous form'susefulness, for example, as an intermediate in the synthesis of a formsuitable for use in pharmaceutical compositions.

There is a need for crystalline forms of Selinexor that arethermodynamically stable and suitable for use in pharmaceuticalcompositions (e.g. are readily dissolvable, exhibit good flowproperties, have desirable particle size distribution and good chemicalstability). There is a further need for crystalline forms of Selinexorhaving physical properties that enable the manufacture of selinexor foruse in pharmaceutical compositions in high yield and high purity.

SUMMARY OF THE INVENTION

The present invention relates to crystalline forms of Selinexor, andcompositions comprising crystalline forms of Selinexor described herein.Selinexor has the chemical structure shown in Structural Formula I:

and is also referred to herein as KG8.

In one embodiment, a single crystalline form of a compound representedby Structural Formula I is provided, wherein the single crystalline formis Form A. In this embodiment, single crystalline Form A ischaracterized by at least three X-ray powder diffraction peaks at 2θangles selected from 4.4°, 19.9°, 21.3° and 22.0°. Single crystallineForm A is the thermodynamically most stable of the forms describedherein.

In another embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form D. In this embodiment, single crystalline FormD is characterized by at least three X-ray powder diffraction peaks at2θ angles selected from 3.7°, 7.3°, 10.9°, 18.3° and 21.9°. Form D isparticularly advantageous when used as an intermediate in thepreparation of From A because it creates a unique ability for high yieldand purity of Selinexor.

In yet another embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form B. In this embodiment, single crystalline FormB is characterized by at least three X-ray powder diffraction peaks at2θ angles selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°.

In another embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form C. In this embodiment, single crystalline FormC is characterized by at least three X-ray powder diffraction peaks at2θ angles selected from 3.7°, 11.2°, 12.1° and 18.6°.

Another embodiment is a composition comprising particles of a singlecrystalline form of a compound represented by Structural Formula I,wherein the single crystalline form is Form A. Single crystalline Form Ais characterized by at least three X-ray powder diffraction peaks at 2θangles selected from 4.4°, 19.9°, 21.3° and 22.0°. In some embodiments,the particles of the composition have a unimodal particle sizedistribution characterized by a d(0.9) of 100 microns or less. In someembodiments, the particles of the composition have a unimodal particlesize distribution characterized by a d(0.9) of 70 microns or less.

Another embodiment is a pharmaceutical composition comprising acomposition comprising particles of single crystalline Form A of acompound of Structural Formula I and a pharmaceutically acceptablecarrier. Single crystalline Form A is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 4.4°, 19.9°,21.3° and 22.0°. In some embodiments, the particles of the compositionhave a unimodal particle size distribution characterized by a d(0.9) of100 microns or less. In some embodiments, the particles of thecomposition have a unimodal particle size distribution characterized bya d(0.9) of 70 microns or less.

The pharmaceutical composition can be used in a method for treating adisorder associated with CRM1 activity (e.g., cancer) or for promotingwound healing in a subject in need thereof. The methods compriseadministering to a subject in need thereof a therapeutically effectiveamount of the pharmaceutical composition.

One embodiment is a method of preparing a single crystalline form of acompound represented by Structural Formula I, wherein the singlecrystalline form is Form A. Single crystalline Form A is characterizedby at least three X-ray powder diffraction peaks at 2θ angles selectedfrom 4.4°, 19.9°, 21.3° and 22.0°. The method comprises suspendingsingle crystalline Form B, C or D of the compound of Structural FormulaI, or or a mixture comprising two or more of the single crystallineforms, in isopropanol or a mixture of isopropanol and water to form aslurry; heating the slurry to a temperature less than or equal to about70° C. to form a second slurry or a solution; cooling the second slurryor the solution and adding water to the second slurry or the solution,thereby forming solid particles of crystalline Form A of the compound ofStructural Formula I; and isolating the solid particles of crystallineForm A. Single crystalline Form D is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 3.7°, 7.3°,10.9°, 18.3° and 21.9°. Single crystalline Form B is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single crystalline Form C ischaracterized by at least three X-ray diffraction peaks at 2θ anglesselected from 3.7°, 11.2°, 12.1° and 18.6°.

Another embodiment is a method of preparing a single crystalline form ofa compound represented by Structural Formula I, wherein the singlecrystalline form is Form A. Single crystalline Form A is characterizedby at least three X-ray powder diffraction peaks at 2θ angles selectedfrom 4.4°, 19.9°, 21.3° and 22.0°. The method comprises suspendingsingle crystalline Form B, C or D of the compound of Structural FormulaI, or or a mixture comprising two or more of the single crystallineforms, in isopropanol or a mixture of isopropanol and water to form aslurry; heating the slurry to a temperature less than or equal to about70° C. to form a second slurry or a solution; adding water to the secondslurry or the solution and cooling the second slurry or the solution,thereby forming solid particles of crystalline Form A of the compound ofStructural Formula I; and isolating the solid particles of crystallineForm A. Single crystalline Form D is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 3.7°, 7.3°,10.9°, 18.3° and 21.9°. Single crystalline Form B is characterized by atlest three X-ray powder diffraction peaks at 2θ angles selected from9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single crystalline Form C ischaracterized by at least three X-ray diffraction peaks at 2θ anglesselected from 3.7°, 11.2°, 12.1° and 18.6°.

Another embodiment is a method of preparing a single crystalline form ofa compound represented by Structural Formula I, wherein the singlecrystalline form is Form A. Single crystalline Form A is characterizedby at least three X-ray powder diffraction peaks at 2θ angles selectedfrom 4.4°, 19.9°, 21.3° and 22.0°. The method comprises heating singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more crystalline forms of the compound ofStructural Formula I and inducing formation of solid particles ofcrystalline Form A; or maturing single crystalline Form B, C or D of thecompound of Structural Formula I, or a mixture comprising two or morecrystalline forms of the compound of Structural Formula I in a solventsystem and inducing formation of solid particles of crystalline Form A;or drying single crystalline Form B, C or D of the compound ofStructural Formula I, or a mixture comprising two or more crystallineforms of the compound of Structural Formula I, thereby forming solidparticles of crystalline Form A; or any combination of the foregoing;and isolating the solid particles of crystalline Form A.

Yet another embodiment is a method of preparing a single crystallineform of a compound represented by Structural Formula I wherein thesingle crystalline form is Form D. Single crystalline Form D ischaracterized by at least three X-ray powder diffraction peaks at 2θangles selected from 3.7°, 7.3°, 10.9°, 18.3° and 21.9°. The methodcomprises dissolving the compound of Structural Formula I in a solventsystem comprising acetonitrile; inducing formation of solid particles ofcrystalline Form D of the compound of Structural Formula I; andisolating the solid particles of crystalline Form D.

In another embodiment, a method of preparing a compound of StructuralFormula I is provided. The method comprises combining a trialkylamine,2-methyltetrahydrofuran, a compound of Structural Formula II:

-   -   and a compound of Structural Formula III:

-   -   to form a reaction mixture        cooling the reaction mixture to from about −80° C. to about 0°        C.; treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I; and isolating the compound of Structural        Formula I from the mixture.

Another embodiment is a method of preparing a single crystalline form ofa compound represented by Structural Formula I wherein the singlecrystalline form is Form A. Single crystalline Form A is characterizedby at least three X-ray powder diffraction peaks at 2θ angles selectedfrom 4.4°, 19.9°, 21.3° and 22.0°. The method comprises combining atrialkylamine, 2-methyltetrahydrofuran, a compound of Structural FormulaII and a compound of Structural Formula III to form a reaction mixture.The reaction mixture is cooled to from about −80° C. to about 0° C. andtreated with propylphosphonic anhydride to provide a mixture comprisingthe compound of Structural Formula I. The compound of Structural FormulaI is isolated from the reaction mixture and the isolated compound ofStructural Formula I is dissolved in a solvent system comprisingacetonitrile. To obtain single crystalline Form D of the compound ofStructural Formula I, formation of solid particles of single crystallineForm D of the compound of Structural Formula I is induced and the solidparticles of crystalline Form D are isolated. In some instances, inaddition to solid particles of crystalline Form D, solid particles ofcrystalline Form B or C of Structural Formula I or a mixture comprisingtwo or more crystalline Forms B, C or D of the compound of StructuralFormula I is isolated. Single crystalline Form D or in some instancessingle crystalline Form B or C of Structural Formula I or a mixturecomprising two or more crystalline Forms B, C or D of the compound ofStructural Formula I is heated and formation of solid particles ofcrystalline Form A is induced; or single crystalline Form D or in someinstances single crystalline Form B or C of Structural Formula I or amixture comprising two or more crystalline Forms B, C or D of thecompound of Structural Formula I is matured in a solvent and formationof solid particles of crystalline Form A is induced; or singlecrystalline Form D or in some instances single crystalline Form B or Cof Structural Formula I or a mixture comprising two or more crystallineForms B, C or D of the compound of Structural Formula I is dried,thereby forming solid particles of crystalline Form A; or anycombination of the foregoing. The solid particles of crystalline Form Aare isolated. Single crystalline Form D is characterized by at leastthree X-ray powder diffraction peaks at 2θ angles selected from 3.7°,7.3°, 10.9°, 18.3° and 21.9°. Single crystalline Form B is characterizedby at least three X-ray powder diffraction peaks at 2θ angles selectedfrom 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single crystalline Form C ischaracterized by at least three X-ray diffraction peaks at 2θ anglesselected from 3.7°, 11.2°, 12.1° and 18.6°.

Another embodiment provides a method for preparing a single crystallineform of a compound represented by Structural Formula I wherein thesingle crystalline form is Form A. Single crystalline Form A ischaracterized by at least three X-ray powder diffraction peaks at 2θangles selected from 4.4°, 19.9°, 21.3° and 22.0°. The method comprisescombining a trialkylamine, 2-methyltetrahydrofuran, a compound ofStructural Formula II and a compound of Structural Formula III to form areaction mixture. The reaction mixture is cooled to from about −80° C.to about 0° C. and treated with propylphosphonic anhydride to provide amixture comprising the compound of Structural Formula I. The compound ofStructural Formula I is isolated from the mixture and dissolved in asolvent system comprising acetonitrile. To obtain single crystallineForm D of the compound of Structural Formula I, formation of solidparticles of crystalline Form D are induced and the solid particles ofcrystalline Form D are isolated. In some instances, in addition to solidparticles of crystalline Form D, solid particles of crystalline Form Bor C of Structural Formula I or a mixture comprising two or morecrystalline Forms B, C or D of the compound of Structural Formula I isisolated Single crystalline Form D, or single crystalline Form B or C ofthe compound of Structural Formula I or a mixture comprising two or morecrystalline forms of the compound of Structural Formula I, is suspendedin isopropanol or a mixture of isopropanol and water to form a slurryand the slurry is heated to a temperature less than or equal to about70° C. to form a second slurry or a solution. The second slurry or thesolution is allowed to cool and water is added, thereby forming solidparticles of crystalline Form A of the compound of Structural Formula I.The solid particles of crystalline Form A are isolated. Singlecrystalline Form D is characterized by at least three X-ray powderdiffraction peaks at 2θ angles selected from 3.7°, 7.3°, 10.9°, 18.3°and 21.9°. Single crystalline Form B is characterized by at lest threeX-ray powder diffraction peaks at 2θ angles selected from 9.4°, 11.1°,16.5°, 18.3° and 18.8°. Single crystalline Form B is characterized by atleast three X-ray diffraction peaks at 2θ angles selected from 3.7°,11.2°, 12.1° and 18.6°. In an alternative embodiment to the foregoing,water is added to the second slurry or solution and then cooling isperformed.

Single crystalline Form D described herein can be prepared, inaccordance with the methods disclosed herein, in high yield and purity.The exceptional purity of single crystalline Form D can be translatedinto highly pure single crystalline Form A for pharmaceutical use, whilethe high yield of single crystalline Form D can be translated into amethod for making single crystalline Form A that is amenable to use on amanufacturing scale e.g., achieving high yield and purity). Using theprocedures for converting single crystalline Form D (or Form B or C)into single crystalline Form A described herein, single crystalline FormA can be isolated as a composition comprising particles of singlecrystalline Form A having a particle size distribution ready forformulation as a pharmaceutical composition (e.g., oral bioavailabilitydespite not being readily dissolvable and/or exhibiting good flowproperties). In addition, of the four forms described herein, Form A isthe thermodynamically most stable form.

BRIEF DESCRIPTION OF THE FIGURES

The foregoing will be apparent from the following more particulardescription of example embodiments of the invention.

FIG. 1A is an X-ray powder diffraction (XRPD) pattern of StructuralFormula I-Form A, prepared in accordance with the procedure described inExample 1.

FIG. 1B is a differential scanning calorimetry (DSC) thermogram and athermogravimetric analysis (TGA) thermogram of Structural Formula I-FormA, prepared in accordance with the procedure described in Example 1.

FIG. 2A is an XRPD pattern of an acetonitrile solvate of StructuralFormula I-Form D, prepared in accordance with the procedure described inExample 7.

FIG. 2B is a DSC thermogram and a TGA thermogram of Structural FormulaI-Form D, prepared in accordance with the procedure described in Example7.

FIG. 3A is an XRPD pattern of Structural Formula I-Form B, prepared inaccordance with the procedure described in Example 7.

FIG. 3B is a DSC thermogram of Structural Formula I-Form B, prepared inaccordance with the procedure described in Example 7.

FIG. 4A is an XRPD pattern of Structural Formula I-Form C, prepared inaccordance with the procedure described in Example 7.

FIG. 4B is a DSC thermogram of Structural Formula I-Form C, prepared inaccordance with the procedure described in Example 7.

FIG. 5A and FIG. 5B show the particle size distribution of Selinexorparticles from Lot No. 1305365, prepared according to the proceduredescribed in Example 1.

FIG. 5C and FIG. 5D show the particle size distribution of Selinexorparticles from Lot No. 1341-AK-109-2, prepared according to theprocedure described in Example 2.

FIG. 5E and FIG. 5F show the particle size distribution of Selinexorparticles from Lot No. PC-14-005, prepared according to the proceduredescribed in Example 3.

FIG. 5G and FIG. 5H show the particle size distribution of Selinexorparticles from Lot No. 1339-BS-142-1, prepared according to theprocedure described in Example 5.

FIG. 5I and FIG. 5J show the particle size distribution of Selinexorparticles from Lot No. 1339-B S-142-2, prepared according to theprocedure described in Example 5.

FIG. 5K and FIG. 5L show the particle size distribution of Selinexorparticles from Lot No. PC-14-008, prepared according to the proceduredescribed in Example 5.

FIG. 5M and FIG. 5N show the particle size distribution of Selinexorparticles from Lot No. PC-14-009, prepared according to the proceduredescribed in Example 4.

FIG. 5O and FIG. 5P shows the particle size distribution of Selinexorparticles from Lot No. 1405463, prepared according to the proceduredescribed in Example 6.

FIG. 5Q is a graph and shows an overlay of the particle sizedistributions depicted in the graphs of FIGS. 5A-5P.

DETAILED DESCRIPTION OF THE INVENTION

A description of example embodiments of the invention follows.

Crystalline Forms of Selinexor

Provided herein are crystalline forms of the compound of StructuralFormula I, designated crystalline Form A, crystalline Form B,crystalline Form C and crystalline Form D.

“Crystalline,” as used herein, refers to a homogeneous solid formed by arepeating, three-dimensional pattern of atoms, ions or molecules (e.g.,an anhydrous molecule or a salt thereof, solvate thereof, or combinationof the foregoing) having fixed distances between constituent parts. Theunit cell is the simplest repeating unit in this pattern.

A crystalline form provided herein can be a single crystalline form orcan comprise a mixture of two or more different crystalline forms. Forexample, in some embodiments, crystalline Forms A, B, C and D of acompound of Structural Formula I are provided as single crystallineforms (i.e., single crystalline Form A, single crystalline Form B,single crystalline Form C, single crystalline Form D). Alternatively, acrystalline form can comprise a mixture of two or more crystalline formsof a compound of Structural Formula I (e.g., a mixture of two or more ofcrystalline Forms A, B, C and D, specifically, two or more ofcrystalline Forms B, C and D).

“Single crystalline form,” as used herein, refers to a single crystal ofa crystalline solid or a plurality of crystals of a crystalline solidwherein each of the plurality of crystals has the same crystal form.

The crystalline forms (e.g., the single crystalline forms) providedherein can be identified on the basis of characteristic peaks in anX-ray powder diffraction (XRPD) analysis. XRPD is a scientific techniquethat measures the X-rays, neutrons or electrons scattered by a powder ormicrocrystalline material as a function of scattering angle. XRPD can beused to identify and characterize crystalline solids, as the diffractionpattern produced by a particular solid is typically distinctive to thatsolid and can be used as a “fingerprint” to identify that solid. Forexample, an XRPD pattern or diffractogram (e.g., a pattern ordiffractogram produced by a sample, such as an unknown sample) that issubstantially in accordance with a reference XRPD pattern ordiffractogram can be used to determine the identity between the samplematerial and the reference material. Both the position and the relativeintensity of the peaks in an XRPD diffractogram are indicative of theparticular phase and identity of a material.

FIGS. 1A, 2A, 3A and 4A show XRPD patterns of various single crystallineforms described herein. An XRPD pattern that is “substantially inaccordance” with one or more figures herein showing an XRPD pattern ordiffractogram is an XRPD pattern that would be considered by one skilledin the art to represent the same single crystalline form of the compoundof Structural Formula I as the sample of the compound of StructuralFormula I that provided the XRPD pattern of one or more figures providedherein. Thus, an XRPD pattern that is substantially in accordance may beidentical to that of one of the figures or, more likely, may be somewhatdifferent from one or more of the figures. An XRPD pattern that issomewhat different from one or more of the figures may not necessarilyshow each of the lines of the diffraction pattern presented hereinand/or may show a slight change in appearance or intensity of the linesor a shift in the position of the lines. These differences typicallyresult from differences in the conditions involved in obtaining the dataor differences in the purity of the sample used to obtain the data. Aperson skilled in the art is capable of determining if a sample of acrystalline compound is of the same form as or a different form from aform disclosed herein by comparison of the XRPD pattern of the sampleand the corresponding XRPD pattern disclosed herein.

It is to be understood that any 2θ angle specified herein, with theexception of the 2θ angles specified in the Figures or theExemplification, means the specified value ±0.2°. For example, when adescribed embodiment or a claim specifies a 2θ of 4.4°, this is to beunderstood to mean 4.4°±0.2°, that is, a 2θ angle of from 4.2° to 4.6°.

The crystalline forms (e.g., the single crystalline forms) providedherein can also be identified on the basis of differential scanningcalorimetry (DSC) and/or thermogravimetric analysis (TGA) DSC is athermoanalytical technique in which the difference in the amount of heatrequired to increase the temperature of a sample is measured as afunction of temperature. DSC can be used to detect physicaltransformations, such as phase transitions, of a sample. For example.DSC can be used to detect the temperature(s) at which a sample undergoescrystallization, melting or glass transition.

TGA is a method of thermal gravimetric analysis in which changes inphysical and chemical properties of a material are measured as afunction of increasing temperature (with constant heating rate) or as afunction of time (with constant temperature anal/or constant mass loss).TGA can provide information about physical phenomena, such assecond-order phase transitions, or about chemical phenomena, such asdesolvation and/or decomposition.

FIGS. 1B, 2B, 3B and 4B show DSC thermograms of various singlecrystalline forms described herein. FIGS. 1B and 2B show TGA thermogramsof various single crystalline forms described herein. A DSC or TGAthermogram that is “substantially in accordance” with one or morefigures herein showing a DSC or TGA thermogram is a DSC or TGAthermogram that would be considered by one skilled in the art torepresent the same single crystalline form of the compound of StructuralFormula I as the sample of the compound of Structural Formula I thatprovided the DSC or TGA thermogram of one or more figures providedherein.

It is to be understood that any temperature associated with DSC or TGAspecified herein, with the exception of the DSC or TGA temperatures inthe Figures or Exemplification, means the specified value ±5° C. orless. For example, when an embodiment or a claim specifies anendothermic peak at about 179° C., this is to be understood to mean 179°C.±5° C. or less, that is a temperature of from 174° C. to 184° C. Inpreferred embodiments, a DSC or TGA temperature is the specified value±3° C., in more preferred embodiments, ±2° C.,

In a first embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form A. Form A is the thermodynamically most stableof the four forms described herein. Single crystalline Form A can becharacterized by at least three X-ray powder diffraction peaks at 2θangles selected from 4.4°, 19.9°, 21.3° and 22.0°. In a particularembodiment, single crystalline Form A is characterized by X-ray powderdiffraction peaks at 2θ angles of 4.4°, 19.9°, 21.3° and 22.0°, moreparticularly, by X-ray powder diffraction peaks at 2θ angles of 4.4°,19.9°, 20.3°, 21.3°, 22.0°, 23.5° and 25.0°, yet more particularly, byX-ray powder diffraction peaks at 2θ angles of 4.4°, 13.1°, 15.8°,18.2°, 19.9°, 20.3°, 21.3°, 22.0°, 23.5°, 23.7°, 25.0°, 27.0°, 28.3° and28.5°. In some embodiments, single crystalline Form A is characterizedby an X-ray powder diffraction pattern substantially in accordance withthat depicted in FIG. 1A.

Single crystalline Form A can be further characterized by a DSCthermogram comprising an endothermic peak at about 179° C. In someembodiments, single crystalline Form A is further characterized by a DSCthermogram and/or a TGA thermogram substantially in accordance with thatdepicted in FIG. 1B.

In a second embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form D. Single crystalline Form D can becharacterized by at least three X-ray powder diffraction peaks at 2θangles selected from 3.7°, 7.3°, 10.9°, 18.3° and 21.9°. In a particularembodiment, single crystalline Form D is characterized by X-ray powderdiffraction peaks at 2θ angles of 3.7°, 7.3°, 10.9°, 18.3° and 21.9°,more particularly, by X-ray powder diffraction peaks at 2θ angles of3.7°, 7.3°, 9.7°, 10.9°, 18.3°, 19.2° and 21.9°, yet more particularly,by X-ray powder diffraction peaks at 2θ angles of 3.7°, 7.3°, 9.7°,10.9°, 11.1°, 18.3°, 19.2°, 19.5°, 20.6° and 21.9°. In some embodiments,single crystalline Form D is characterized by an X-ray powderdiffraction pattern substantially in accordance with that depicted in.FIG. 2A.

Single crystalline Form D can be further characterized by a DSCthermogram and/or a TGA thermogram substantially in accordance with thatdepicted in FIG. 2B. The DSC thermogram indicates multiple melting andrecrystallizing events indicating interconversion of forms.

In some embodiments, single crystalline Form D is in the form of asolvate, for example, an acetonitrile solvate. In some embodiments, thesolvate (e.g., acetonitrile solvate) comprises from about 0.5 to about1.5 molar equivalents of solute (e.g., acetonitrile) per molarequivalent of the compound of Structural Formula I, more particularly,one molar equivalent of solute per molar equivalent of the compound ofStructural Formula I.

“Solvate,” as used herein, refers to a chemical compound formed by theinteraction of a solute (e.g., a compound of Structural Formula I) andone or more solvents (e.g., acetonitrile, water). Thus, “solvate”includes solvates containing a single type of solvent molecule andsolvates containing more than one type of solvent molecule (mixedsolvates). Typically, the one or more solvents in solvates describedherein is an organic solvent or a combination of organic solvents,although water can also form solvates, called hydrates. Exemplarysolvates include acetonitrile solvates.

In a third embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form B. Single crystalline Form B can becharacterized by at least three X-ray powder diffraction peaks at 2θangles selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. In aparticular embodiment, single crystalline Form B is characterized byX-ray powder diffraction peaks at 2θ angles of 9.4°, 11.1°, 16.5°, 18.3°and 18.8°, more particularly, by X-ray powder diffraction peaks at 2θangles of 9.4°, 11.1°, 16.5°, 18.3°, 18.8°, 20.2° and 20.8°, yet moreparticularly, by X-ray powder diffraction peaks at 2θ angles of 8.1°,9.4°, 11.1°, 13.8°, 16.5°, 18.3°, 18.8°, 20.2° and 20.8°. In someembodiments, single crystalline Form B is characterized by an X-raypowder diffraction pattern substantially in accordance with thatdepicted in FIG. 3A.

Single crystalline Form B can be further characterized by a DSCthermogram and/or a TGA thermogram substantially in accordance with thatdepicted in FIG. 3B. The DSC thermogram indicates multiple melting andrecrystallizing events indicating interconversion of forms.

In a fourth embodiment, a single crystalline form of a compoundrepresented by Structural Formula I is provided, wherein the singlecrystalline form is Form C. Single crystalline Form C can becharacterized by at least three X-ray powder diffraction peaks at 2θangles selected from 3.7°, 11.2°, 12.1° and 18.6°. In a particularembodiment, single crystalline Form C is characterized by X-ray powderdiffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1° and18.6°, more particularly, by X-ray powder diffraction peaks at 2θ anglesselected from 3.7°, 11.2°, 17.7°, 12.1°, 18.6°, 19.7°, 21.2° and 22.2°.In some embodiments, single crystalline Form C is characterized by anX-ray powder diffraction pattern substantially in accordance with thatdepicted in FIG. 4A.

Single crystalline Form D can be further characterized by a DSCthermogram and/or a TGA thermogram substantially in accordance with thatdepicted in FIG. 4B. The DSC thermogram indicates multiple melting andrecrystallizing events indicating intercoversion of forms.

Compositions

Also provided herein are compositions comprising particles (e.g., solidparticles) of a single crystalline form (e.g., Form A, B, C or D) of acompound of Structural Formula I, wherein characteristics andalternative characteristics of the single crystalline form in thecomposition, including alternative XRPD, DSC and/or TGA characteristics,are as described above with respect to the first through fourthembodiments.

A fifth embodiment is a composition comprising particles (e.g., solidparticles) of a single crystalline form of a compound represented byStructural Formula I, wherein the single crystalline form is Form A.Single crystalline Form A is characterized by at least three X-raypowder diffraction peaks at 2θ angles selected from 4.4°, 19.9°, 21.3°and 22.0°. In some embodiments, the particles of the composition have aparticle size distribution (e.g., unimodal particle size distribution)characterized by a d(0.9) of 100 microns or less. In some embodiments,the particles of the composition have a particle size distribution(e.g., a unimodal particle size distribution) characterized by a d(0.9)of 70 microns or less.

“Particle size distribution,” as used herein, refers to a list of valuesor a mathematical function that defines the relative amount, typicallyby mass or volume, of particles present in a sample according to size.Particle size distribution can be characterized by one or more values,such as d(0.9), d(0.5) or d(0.1) or a ratio of any of the foregoing, orby the shape of the mathematical function, when graphed. Exemplaryshapes of a particle size distribution graph include unimodal, bimodal,normal and Gaussian.

“d(0.9),” as used herein, describes the value of particle size at which90% of the total volume of particles is comprised of particles no largerthan the indicated size. d(0.9) is used interchangeably herein with d90,d₉₀ and D90. It is to be understood that any d(0.9) value specifiedherein, with the exception of the d(0.9) values in the Figures or theExemplification, means the specified value ±15% or less of the specifiedvalue, in preferred embodiments, d(0.9) is the specified value ±10% Forexample, when an embodiment or a claim specifies a d(0.9) of about 70microns, this is to be understood to mean 70 microns±7 microns, that isfrom 63 microns to 77 microns. In preferred embodiments, d(0.9) is thespecified value ±7.5%, more preferably, ±6.5%. Similarly, when anembodiment or a claim specifies a d(0.9) of about 100 microns, this isto be understood for example of the specified value being ±10%, to mean100 microns ±10 microns, that is from 90 microns to 110 microns.

“d(0.5),” as used herein, describes the value of particle size at which50% of the total volume of particles is comprised of particles no largerthan the indicated size. d(0.5) is typically the median of the particlesize distribution, d(0.5) is used interchangeably herein with d50, d₅₀and D50. It is to be understood that any d(0.5) value specified herein,with the exception of the d(0.5) values in the Figures or theExemplification, means the specified value ±10% or less of the specifiedvalue. For example, when an embodiment or a claim specifies a d(0.5) ofabout 25 microns, this is to be understood to mean 25 microns±2.5microns, that is front 22.5 microns to 27.5 microns. In preferredembodiments, d(0.5) is the specified value ±5%, more preferably, ±2.5%,yet more preferably, ±1.5%.

“d(0.1),” as used herein, describes the value of particle size at which10% of the total volume of particles is comprised of particles no largerthan the indicated size. d(0.1) is used interchangeably herein with d10,d₁₀ and D10. It is to be understood that any d(0.1) value specifiedherein, with the exception of the d(0.1) values in the Figures or theExemplification, means the specified value ±30% or less of the specifiedvalue. For example, when an embodiment or a claim specifies a d(0.1) ofabout 10 microns, this is to be understood to mean 10 microns±3.0microns, that is from 7 microns to 13 microns. In preferred embodiments,d(0.1) is the specified value ±15%, more preferably, ±5%, yet morepreferably, ±3%.

It is to be understood that any ratio of d(0.9):d(0.1) or d(0.9):d(0.5)specified herein, with the exception of the d(0.9):d(0.1) ord(0.9):d(0.5) ratios in the Figures or Exemplification, means thespecified value ±15% or less of the specified value. For example, whenan embodiment or a claim specifies a ratio of d(0.9):d(0.1) of about 10,this is to be understood to mean 10±1.5, that is from 8.5 to 11.5. Inpreferred embodiments, the d(0.9):d(0.1) or d(0.9):d(0.5) ratio is thespecified value ±10%, more preferably, ±5%.

In a first aspect of the fifth embodiment, the particles have a particlesize distribution characterized by a d(0.9) of from 10 microns to 100microns, specifically, a d(0.9) of from 25 microns to 100 microns, morespecifically, a d(0.9) of from 60 microns to 100 microns.

In a second aspect of the fifth embodiment, the particles have aparticle size distribution characterized by a d(0.9) of from 10 micronsto 70 microns, specifically, a d(0.9) of from 25 microns to 70 microns,more specifically, a d(0.9) of from 60 microns to 70 microns.

In a third aspect of the fifth embodiment, the particles have a particlesize distribution characterized by a d(0.5) of from 10 microns to 35microns, specifically, a d(0.5) of from 15 microns to 30 microns, morespecifically, a d(0.5) of from 25 to 30 microns. Values and alternativevalues for d(0.9) are as described in the fifth embodiment, or firstaspect thereof.

In a fourth aspect of the fifth embodiment, the particles have aparticle size distribution characterized by a d(0.1) of 5 microns orgreater, more specifically, 10 microns or greater. For example, in someaspects, the particles have a particle size distribution characterizedby a d(0.1) of from 5 microns to 15 microns, more specifically, from 10microns to 15 microns. Values and alternative values for d(0.9) andd(0.5) are as described in the fifth embodiment, or first or secondaspect thereof.

In a fifth aspect of the fifth embodiment, the particles have a particlesize distribution characterized by a d(0.9):d(0.1) ratio of 10 or less,specifically, of 7.5 or less, more specifically, of 6 or less. Forexample, in some aspects, the particles have a particle sizedistribution characterized by a d(0.9):d(0.1) ratio of from 2 to 10,specifically, of from 5 to 7.5 or, more specifically, of from 5 to 6.Values and alternative values for d(0.9), d(0.5) and d(0.1) are asdescribed in the fifth embodiment, or first, second or third aspect ofthe foregoing.

In a sixth aspect of the fifth embodiment, the particles have a particlesize distribution characterized by a d(0.9):d(0.5) ratio of 4.5 or less,more specifically, of 3 or less. For example, in some aspects, theparticles have a particle size distribution characterized by ad(0.9):d(0.5) ratio of from 1.5 to 4.5 or, more specifically, of from 2to 3. Values and alternative values for d(0.9), d(0.5) and d(0.1), andratios thereof, are as described in the fifth embodiment, or first,second, third or fourth aspect of the foregoing.

In a seventh aspect of the fifth embodiment, the particles have aunimodal particle size distribution, for example, a normal particle sizedistribution. Values and alternative values for d(0.9), d(0.5) andd(0.1), and ratios thereof, are as described in the fifth embodiment, orfirst through fifth aspects of the foregoing.

“Unimodal,” used herein in connection with particle size distribution,refers to a particle size distribution that, when graphed, contains asingle local maxima. An exemplary unimodal particle size distributioncan be found in FIG. 5K.

As used herein, “normal particle size distribution” refers to a particlesize distribution that obeys a function that represents the distributionof particle sizes in a sample as a symmetrical or substantiallysymmetrical bell-shaped graph. At least FIG. 5K depicts a normalparticle size distribution.

Alternative characteristics of single crystalline Form A in acomposition of the fifth embodiment, including alternative XRPD, DSCand/or TGA characteristics, are as described above with respect to thefirst embodiment.

Pharmaceutical Compositions

Also provided herein are pharmaceutical compositions comprising a singlecrystalline form (e.g., Form A, B, C or D) or composition describedherein and a pharmaceutically acceptable carrier. The compositioncomprises particles of a single crystalline form (e.g., Form A, B, C orD) of a compound of Structural Formula I. Characteristics andalternative characteristics of the single crystalline form, includingalternative XRPD, DSC and/or TGA characteristics, arc as described abovewith respect to the first through fourth embodiments.

A sixth embodiment is a pharmaceutical composition comprising acomposition (e.g., a composition of the fifth embodiment, or any aspectthereof) comprising particles of single crystalline Form A of a compoundof Structural Formula I and a pharmaceutically acceptable carrier.Characteristics and alternative characteristics of single crystallineForm A, including alternative XRPD, DSC and/or TGA characteristics, areas described above with respect to the first embodiment. Values andalternative values for d(0.9), d(0.5) and d(0.1), and ratios thereof, aswell as characteristics of the particle size distribution (e.g.,unimodal, normal) of the particles of single crystalline Form A, are asdescribed in the fifth embodiment, of any aspect thereof.

The term “pharmaceutically acceptable carrier” means a non-toxicsolvent, dispersant, excipient, adjuvant or other material which ismixed with the active ingredient in order to permit formation of apharmaceutical composition, i.e., a dosage form capable of beingadministered to a subject. A “pharmaceutically acceptable carrier”should not destroy the activity of the compound with which it isformulated. Pharmaceutically acceptable carriers are well known in theart.

Pharmaceutically acceptable carriers, adjuvants or vehicles that may beused in the pharmaceutical compositions of this invention include, butare not limited to, ion exchangers, alumina, aluminum stearate,lecithin, serum proteins, such as human serum albumin, buffer substancessuch as phosphates, glycine, sorbic acid, potassium sorbate, partialglyceride mixtures of saturated vegetable fatty acids, water, salts orelectrolytes, such as protamine sulfate, disodium hydrogen phosphate,potassium hydrogen phosphate, sodium chloride, zinc salts, colloidalsilica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-basedsubstances, polyethylene glycol, sodium carboxymethylcellulose,polyacrylates, waxes, polyethylene-polynxypropylene-block polymers,polyethylene glycol and wool fat.

Pharmaceutical compositions of the present invention may be administeredorally, parenterally (including subcutaneous, intramuscular, intravenousand intradermal), by inhalation spray, topically, rectally, nasally,buccally, vaginally or via an implanted reservoir. In some embodiments,provided pharmaceutical compositions are administrable intravenouslyand/or intraperitoneally.

The term “parenteral” as used herein includes subcutaneous, intravenous,intramuscular, intraocular, intravitreal, intra-articular,intra-synovial, intrastemal, intrathecal, intrahepatic, intraperitonealintralesional and intracranial injection or infusion techniques.Preferably, the pharmaceutical compositions are administered orally,subcutaneously, intraperitoneally or intravenously. Sterile injectableforms of the pharmaceutical compositions of this invention may beaqueous or oleaginous suspension. These suspensions may be formulatedaccording to techniques known in the art using suitable dispersing orwetting agents and suspending agents. The sterile injectable preparationmay also be a sterile injectable solution or suspension in a non-toxicparenterally acceptable diluent or solvent, for example as a solution in1,3-butanediol. Among the acceptable vehicles and solvents that may beemployed are water, Ringer's solution and isotonic sodium chloridesolution. In addition, sterile, fixed oils are conventionally employedas a solvent or suspending medium.

Pharmaceutical compositions of this invention may be orally administeredin any orally acceptable dosage form including, but not limited to,capsules, tablets, aqueous suspensions or solutions. In the case oftablets for oral use, carriers commonly used include lactose and cornstarch. Lubricating agents, such as magnesium stearate, are alsotypically added. For oral administration in a capsule form, usefuldiluents include lactose and dried cornstarch. When aqueous suspensionsare required for oral use, the active ingredient is combined withemulsifying and suspending agents. If desired, certain sweetening,flavoring or coloring agents may also be added. In some embodiments, aprovided oral formulation is formulated for immediate release orsustained/delayed release. In some embodiments, the composition issuitable for buccal or sublingual administration, including tablets,lozenges and pastilles. A provided compound can also be inmicro-encapsulated form.

Specific pharmaceutically acceptable carriers suitable for use in anoral formulation such as a tablet or capsule include, but are notlimited to, microcrystalline cellulose (Avicel PH101), croscarmelloseSodium (Ac-Di-Sol), kollidon 30 powder (polyvinylpyrrolidone, povidone),colloidal silicon dioxide M5-P, magnesium stearate, microcrystallinecellulose (Avcel PH102), sodium lauryl sulfate (Kolliphor SLS Fine) andColloidal Silicon Dioxide M5-P. Each of the above listed carriers can beused in an oral formulation either alone or in any combination.

Alternatively, pharmaceutical compositions of this invention may beadministered in the form of suppositories for rectal administration.Pharmaceutical compositions of this invention may also be administeredtopically, especially when the target of treatment includes areas ororgans readily accessible by topical application, including diseases ofthe eye, the skin, or the lower intestinal tract. Suitable topicalformulations are readily prepared for each of these areas or organs.

Topical application for the lower intestinal tract can be effected in arectal suppository formulation (see above) or in a suitable enemaformulation. Topically-transdermal patches, ointments, creams, foams andgels may also be used. Specific carriers for use in topical formulationsinclude, but are not limited to, 2-hydroxypropyl beta-cyclodextrin(HPBCD), methylcellulose, sodium benzoate, water and glycerin.

For ophthalmic use, provided pharmaceutical compositions may beformulated as micronized suspensions or in an ointment such aspetrolatum.

Pharmaceutical compositions of this invention may also be administeredby nasal aerosol or inhalation.

In some embodiments, pharmaceutical compositions of this invention areformulated for intra-peritoneal administration.

The amount of compound of Structural Formula I (e.g., single crystallineForm A of the compound of Structural Formula I) in pharmaceuticalcompositions of this invention is such that is effective to measurablyinhibit CRM1, in a biological sample or in a subject. In certainembodiments, a pharmaceutical composition of this invention isformulated for administration to a subject in need of suchpharmaceutical composition. The term “subject,” as used herein, means ananimal. In some embodiments, the animal is a mammal. In certainembodiments, the subject is a veterinary patient (i.e., a non-humanmammal patient, such as a dog, a cat, a horse, a pig or a rodent, suchas a mouse or rat). In some embodiments, the subject is a dog. In otherembodiments, she subject is a human (e.g., a human patient).

The amount of compound of Structural Formula I (e.g., single crystallineForm A of the compound of Structural Formula I) that may be combinedwith the pharmaceutically acceptable carrier materials to produce apharmaceutical composition in a single dosage form will vary dependingupon the host treated and/or the particular mode of administration. Inone embodiment, provided pharmaceutical compositions should beformulated so that a dosage of between 0.01-100 mg/kg body weight/day ofthe compound of Structural Formula I can be administered to a patientreceiving these compositions. In another embodiment, the dosage is fromabout 0.5 to about 100 mg/kg of body weight, or between 1 mg and 1000mg/dose, every 4 to 120 hours, or according to the requirements of theparticular drug. Typically, the pharmaceutical compositions of thisinvention will be administered from about 1 to about 6 times per day.

It should also be understood that a specific dosage and treatmentregimen for any particular subject (e.g., patient) will depend upon avariety of factors, including the activity of the specific compoundemployed, the age, body weight, general health, sex, diet, time ofadministration, rate of excretion, drug combination, and the judgment ofthe treating physician and the severity of the particular disease beingtreated.

Upon improvement of a subject's condition, a maintenance dose of apharmaceutical composition of this invention may be administered, ifnecessary. Subsequently, the dosage or frequency of administration, orboth, may be reduced, as a function of the symptoms, to a level at whichthe improved condition is retained when the symptoms have beenalleviated to the desired level. Subjects may, however, requireintermittent treatment on a long-term basis upon any recurrence ofdisease symptoms.

Methods of Treatment and Uses for Pharmaceutical Compositions

Pharmaceutical compositions and compounds described herein are generallyuseful for the inhibition of CRM1 and are, therefore, useful fortreating one or more disorders associated with activity of CRM1. Thus,in certain embodiments, the present invention provides a method fortreating a disorder associated with CRM1 activity, comprisingadministering to a subject in need thereof a therapeutically effectiveamount of a pharmaceutical composition described herein. The compound ofStructural Formula I or (single) crystalline form thereof,pharmaceutical composition thereof or combination of the foregoing canalso be administered to cells in culture, e.g., in vitro or ex vivo, orto a subject, e.g., in vivo, to treat, prevent, and/or diagnose avariety of disorders, including those described hereinbelow.

The activity of a compound Structural Formula I, or (single) crystallineform thereof, pharmaceutical composition thereof, or combination of theforegoing as an inhibitor of CRM1 may be assayed in vitro, in vivo or ina cell line. Detailed conditions for assaying a compound of StructuralFormula I as an inhibitor of CRM1 are set forth in InternationalPublication No. WO 2013/019548.

The term “treat” or “treating” means to alleviate symptoms, eliminatethe causation of the symptoms, either on a temporary or permanent basis,or to prevent or slow the appearance of symptoms of the named disorderor condition.

The term “CRM1-mediated” disorder or condition or “disorder associatedwith CRM1 activity,” as used herein, means any disease or otherdeleterious condition in which CRM1 is known to play a role.Accordingly, another embodiment of the present invention relates totreating or lessening the severity of one or more diseases in which CRM1is known to play a role. In some embodiments, the present inventionprovides methods of treating a disease associated with expression oractivity of p53, p73, p21, pRB, p27, IκB, NFκB, c-Abl, FOXO proteins,COX-2, or an HDAC (historic deacetylases) in a subject comprisingadministering to the subject a therapeutically effective amount of apharmaceutical composition described herein. In another embodiment, thepresent invention relates to a method of treating or lessening theseverity of a disease or condition selected from a proliferativedisorder (e.g., cancer), an inflammatory disorder, an autoimmunedisorder, a viral infection, an ophthalmological disorder or aneurodegenerative disorder wherein said method comprises administeringto a patient in need thereof a compound or composition according to thepresent invention. In a more specific embodiment, the present inventionrelates to a method of treating or lessening the severity of cancer.Specific examples of the above disorders are set forth in detail below.

The term “therapeutically effective amount” means an amount of thecompound of Structural Formula I or (single) crystalline form thereof(typically, in a pharmaceutical composition described herein) which iseffective in treating or lessening the severity of one or more symptomsof a disorder or condition. In the case of promoting wound healing, atherapeutically effective amount is an amount of the compound ofStructural Formula I or (single) crystalline form thereof (typically, ina pharmaceutical composition described herein) that promotes healing ofa wound.

As used herein, “promoting wound healing” means treating a subject witha wound and achieving healing, either partially or fully, of the wound.Promoting wound healing can mean, e.g., one or more of the following:promoting epidermal closure; promoting migration of the dermis;promoting dermal closure in the dermis; reducing wound healingcomplications, e.g., hyperplasia of the epidermis and adhesions;reducing wound dehiscence; and promoting proper scab formation.

Cancers treatable by the pharmaceutical compositions or compounds ofthis invention include, but are not limited to, hematologic malignancies(leukemias, lymphomas, myelomas including multiple myeloma,myelodysplastic and myeloproliferative syndromes) and solid tumors(carcinomas such as prostate, breast, lung, colon, pancreatic, renal,ovarian as well as soft tissue and osteosarcomas, and stromal tumors).Breast cancer (BC) can include basal-like breast cancer (BLBC), triplenegative breast cancer (TNBC) and breast cancer that is both BLBC andTNBC. In addition, breast cancer can include invasive or non-invasiveductal or lobular carcinoma, tubular, medullary, mucinous, papillary,cribriform carcinoma of the breast, male breast cancer, recurrent ormetastatic breast cancer, phyllodes tumor of the breast and Paget'sdisease of the nipple.

Inflammatory disorders treatable by the pharmaceutical compositions orcompounds of this invention include, but are not limited to, multiplesclerosis, rheumatoid arthritis, degenerative joint disease, systemiclupus, systemic sclerosis, vasculitis syndromes (small, medium and largevessel), atherosclerosis, inflammatory bowel disease, irritable bowelsyndrome, Crohn's disease, mucous colitis, ulcerative colitis,gastritis, sepsis, psoriasis and other dermatological inflammatorydisorders (such as eczema, atopic dermatitis, contact dermatitis,urticaria, scleroderma, and dermatosis with acute inflammatorycomponents, pemphigus, pemphigoid, allergic dermatitis), and urticarialsyndromes.

Viral diseases treatable by the pharmaceutical compositions or compoundsof this invention include, but are not limited to, acute febrilepharyngitis, pharyngoconjunctival fever, epidemic keratoconjunctivitis,infantile gastroenteritis, Coxsackie infections, infectiousmononucleosis, Burkitt lymphoma, acute hepatitis, chronic hepatitis,hepatic cirrhosis, hepatocellular carcinoma, primary HSV-1 infection(e.g., gingivostomatitis in children, tonsillitis and pharyngitis inadults, keratoconjunctivitis), latent HSV-1 infection (e.g., herpeslabialis and cold sores), primary HSV-2 infection, latent HSV-2infection, aseptic meningitis, infectious mononucleosis, Cytomegalicinclusion disease, Kaposi's sarcoma, multicentric Castleman disease,primary effusion lymphoma, AIDS, influenza, Reye syndrome, measles,postinfectious encephalomyelitis, Mumps, hyperplastic epithelial lesions(e.g., common, flat, plantar and anogenital warts, laryngeal papillomas,epidermodysplasia verruciformis), cervical carcinoma, squamous cellcarcinomas, croup, pneumonia, bronchiolitis, common cold, Poliomyelitis,Rabies, influenzae-like syndrome, severe bronchiolitis with pneumonia,German measles, congenital rubella, Varicella, and herpes zoster. Viraldiseases treatable by the compounds of this invention also includechronic viral infections, including hepatitis B and hepatitis C.

Exemplary ophthalmology disorders include, but are not limited to,macular edema (diabetic and nondiabetic macular edema), aged relatedmacular degeneration wet and dry forms, aged disciform maculardegeneration, cystoid macular edema, palpebral edema, retina edema,diabetic retinopathy, chorioretinopathy, neovascular maculopathy,neovascular glaucoma, uveitis, iritis, retinal vasciditis,endophthalmitis, panophthalmitis, metastatic ophthalmia, choroiditis,retinal pigment epitheliitis, conjunctivitis, cyclitis, scleritis,episcleritis, optic neuritis, retrobulbar optic neuritis, keratitis,blepharitis, exudative retinal detachment, corneal ulcer, conjunctivalulcer, chronic nummular keratitis, ophthalmic disease associated withhypoxia or ischemia, retinopathy of prematurity, proliferative diabeticretinopathy, polypoidal choroidal vasculopathy, retinal angiomatousproliferation, retinal artery occlusion, retinal vein occlusion, Coats'disease, familial exudative vitreoretinopathy, pulseless disease(Takayasu's disease), Eales disease, antiphospholipid antibody syndrome,leukemic retinopathy, blood hyperviscosity syndrome, macroglobulinemia,interferon-associated retinopathy, hypertensive retinopathy, radiationretinopathy, corneal epithelial stem cell deficiency or cataract.

Neurodegenerative diseases treatable by pharmaceutical compositions orcompounds of the invention include, but are not limited to, Parkinson's,Alzheimer's, and Huntington's, and Amyotrophic lateral sclerosis(ALS/Lou Gehrig's Disease).

Pharmaceutical compositions or compounds described herein may also beused to treat disorders of abnormal tissue growth and fibrosis includingdilative cardiomyopathy, hypertrophic cardiomyopathy, restrictivecardiomyopathy, pulmonary fibrosis, hepatic fibrosis,glomerulonephritis, polycystic kidney disorder (PKD) and other renaldisorders.

Pharmaceutical compositions or compounds described herein may also beused to treat disorders related to food intake such as obesity andhyperphagia.

In another embodiment, a pharmaceutical composition or compoundsdescribed herein may be used to treat or prevent allergies andrespiratory disorders, including asthma, bronchitis, pulmonary fibrosis,allergic rhinitis, oxygen toxicity, emphysema, chronic bronchitis, acuterespiratory distress syndrome, and any chronic obstructive pulmonarydisease (COPD).

In some embodiments, the disorder or condition associated with CRM1activity is beta-thalassemia, muscular dystrophy, arthritis, forexample, osteoarthritis and rheumatoid arthritis, ankylosingspondilitis, traumatic brain injury, spinal cord injury, sepsis,rheumatic disease, cancer atherosclerosis, type 1 diabetes, type 2diabetes, leptospiriosis renal disease, glaucoma, retinal disease,ageing, headache, pain, complex regional pain syndrome, cardiachypertrophy, musclewasting, catabolic disorders, obesity, fetal growthretardation, hypercholesterolemia, heart disease, chronic heart failure,ischemic/reperfusion, stroke, cerebral aneurysm, angina pectoris,pulmonary disease, cystic fibrosis, acid-induced lung injury, pulmonaryhypertension, asthma, chronic obstructive pulmonary disease, Sjogren'ssyndrome, hyaline membrane disease, kidney disease, glomerular disease,alcoholic liver disease, gut diseases, peritoneal endometriosis, skindiseases, nasal sinusitis, mesothelioma, anhidrotic ecodernialdysplasia-ID, behcet's disease, incontinentia pigmenti, tuberculosis,asthma, crohn's disease, colitis, ocular allergy, appendicitis, paget'sdisease, pancreatitis, periodonitis, endometriosis, inflammatory boweldisease, inflammatory lung disease, silica-induced diseases, sleepapnea, AIDS, HIV-1, autoimmune diseases, antiphospholipid syndrome,lupus, lupus nephritis, familial mediterranean fever, hereditaryperiodic fever syndrome, psychosocial stress diseases, neuropathologicaldiseases, familial amyloidotic polyneuropathy, inflammatory neuropathy,parkinson's disease, multiple sclerosis, alzheimer's disease,anlyotropic lateral sclerosis, huntington's disease, cataracts, orhearing loss.

In other embodiments, the disorder or condition associated with CRM1activity is head injury, uveitis, inflammatory pain, allergen inducedasthma, non-allergen induced asthma, glomerular nephritis, ulcerativecolitis, necrotizing enterocolitis, hyperimmunoglobulinemia D withrecurrent fever (HIDS), TNF receptor associated periodic syndrome(TRAPS), cryopyrin-associated periodic syndromes, Muckle-Wells syndrome(urticaria deafness amyloidosis), familial cold urticaria, neonatalonset multisystem inflammatory disease (NOMID), periodic fever, aphthousstomatitis, pharyngitis and adenitis (PFAPA syndrome), Blau syndrome,pyogenic sterile arthritis, pyoderma gangrenosum, acne (PAPA),deficiency of the interleukin-1-receptor antagonist (DIRA), subarachnoidhemorrhage, polycystic kidney disease, transplant, organ transplant,tissue transplant, myelodysplastic syndrome, irritant-inducedinflammation, plant irritant-induced inflammation, poison ivy/urushioloil-induced inflammation, chemical irritant-induced inflammation, beesting-induced inflammation, insect bite-induced inflammation, sunburn,burns, dermatitis, endotoxemia, lung injury, acute respiratory distresssyndrome, alcoholic hepatitis, or kidney injury caused by parasiticinfections.

In further aspects, the present invention provides a use of apharmaceutical composition or compounds described herein for themanufacture of a medicament for the treatment of a disorder associatedwith CRM1 activity. The present invention also provides a pharmaceuticalcomposition described herein for use in treating a disorder associatedwith CRM1 activity. Specific examples of disorders associated with CRM1activity are as set forth in detail herein.

In yet further aspects, the present invention provides a use of apharmaceutical composition or compounds described herein for themanufacture of a medicament for the treatment of a disease associatedwith expression or activity of p53, p73, p21, pRB, p27, IκB, NFκB,c-Abl, FOXO proteins, COX-2 or an HDAC in a subject. In someembodiments, the present invention provides a use of a pharmaceuticalcomposition described herein in the manufacture of a medicament for thetreatment of any of cancer and/or neoplastic disorders, angiogenesis,autoimmune disorders, inflammatory disorders and/or diseases,epigenetics, hormonal disorders and/or diseases, viral diseases,neurodegenerative disorders and/or diseases, wounds, and ophthalmologicdisorders.

In some embodiments, the present invention provides a method forinhibiting CRM1 in a biological sample comprising contacting thebiological sample with, or administering to the patient, apharmaceutical composition of the invention.

Neoplastic Disorders

A pharmaceutical composition or compound described herein can be used totreat a neoplastic disorder. A “neoplastic disorder” is a disease ordisorder characterized by cells that have the capacity for autonomousgrowth or replication, e.g., an abnormal state or conditioncharacterized by proliferative cell growth. Exemplary neoplasticdisorders include: carcinoma, sarcoma, metastatic disorders, e.g.,tumors arising from prostate, brain, bone, colon, lung, breast, ovarian,and liver origin, hernatopoietic neoplastic disorders, e.g., leukemias,lymphomas, myeloma and other malignant plasma cell disorders, andmetastatic tumors. Prevalent cancers include: breast, prostate, colon,lung, liver, and pancreatic cancers. Treatment with the compound can bein an amount effective to ameliorate at least one symptom of theneoplastic disorder, e.g., reduced cell proliferation., reduced tumormass, etc.

The disclosed methods are useful in the prevention and treatment ofcancer, including for example, solid tumors, soft tissue tumors, andmetastases thereof, as well as in familial cancer syndromes such as LiFraumeni Syndrome, Familial Breast-Ovarian Cancer (BRCA1 or BRAC2mutations) Syndromes, and others. The disclosed methods are also usefulin treating non-solid cancers. Exemplary solid tumors includemalignancies (e.g., sarcomas, adenocarcinomas, and carcinomas) of thevarious organ systems, such as those of lung, breast, lymphoid,gastrointestinal (e.g., colon), and genitourinary (e.g., renal,urothelial, or testicular tumors) tracts, pharynx, prostate, and ovary.Exemplary adenocarcinomas include colorectal cancers, renal-cellcarcinoma, liver cancer, non-small cell carcinoma of the lung, andcancer of the small intestine.

Exemplary cancers described by the National Cancer Institute include:Acute Lymphoblastic Leukemia, Adult; Acute Lymphoblastic Leukemia,Childhood; Acute Myeloid Leukemia, Adult; Adrenocortical Carcinoma;Adrenocortical Carcinoma, Childhood; AIDS-Related Lymphoma; AIDS-RelatedMalignancies; Anal Cancer; Astrocytoma, Childhood Cerebellar;Astrocytoma, Childhood Cerebral; Bile Duct Cancer, Extrahepatic; BladderCancer; Bladder Cancer, Childhood; Bone Cancer, Osteosarcoma/MalignantFibrous Histiocytoma; Brain Stem Glioma, Childhood; Brain Tumor, Adult;Brain Tumor, Brain Stem Glioma, Childhood; Brain Tumor, CerebellarAstrocytoma, Childhood; Brain Tumor, Cerebral Astrocytoma/MalignantGlioma, Childhood; Brain Tumor, Ependymoma, Childhood; Brain Tumor,Medulloblastoma, Childhood; Brain Tumor, Supratentorial PrimitiveNeuroectodermal Tumors, Childhood; Brain Tumor, Visual Pathway andHypothalamic Glioma, Childhood; Brain Tumor, Childhood (Other); BreastCancer; Breast Cancer and Pregnancy; Breast Cancer, Childhood; BreastCancer, Male; Bronchial Adenomas/Carcinoids, Childhood; Carcinoid Tumor,Childhood; Carcinoid Tumor, Gastrointestinal; Carcinoma, Adrenocortical;Carcinoma, Islet Cell; Carcinoma of Unknown Primary; Central NervousSystem Lymphoma, Primary; Cerebellar Astrocytoma, Childhood; CerebralAstrocytoma/Malignant Glioma, Childhood; Cervical Cancer; ChildhoodCancers; Chronic Lymphocytic Leukemia; Chronic Myelogenous Leukemia;Chronic Myeloproliferative Disorders; Clear Cell Sarcoma of TendonSheaths; Colon Cancer; Colorectal Cancer, Childhood; Cutaneous T-CellLymphoma; Endometrial Cancer; Ependymoma, Childhood; Epithelial Cancer,Ovarian; Esophageal Cancer; Esophageal Cancer, Childhood; Ewing's Familyof Tumors; Extracranial Germ Cell Tumor, Childhood; Extragonadal GermCell Tumor; Extrahepatic Bile Duct Cancer; Eye Cancer, IntraocularMelanoma; Eye Cancer, Retinoblastoma; Gallbladder Cancer; Gastric(Stomach) Cancer; Gastric (Stomach) Cancer, Childhood; GastrointestinalCarcinoid Tumor; Germ Cell Tumor, Extracranial, Childhood; Germ CellTumor, Extragonadal; Germ Cell Tumor, Ovarian; Gestational TrophoblasticTumor; Glioma, Childhood Brain Stem; Glioma, Childhood Visual Pathwayand Hypothalamic; Hairy Cell Leukemia; Head and Neck Cancer;Hepatocellular (Liver) Cancer, Adult (Primary); Hepatocellular (Liver)Cancer, Childhood (Primary); Hodgkin's Lymphoma, Adult; Hodgkin'sLymphoma, Childhood; Hodgkin's Lymphoma During Pregnancy; HypopharyngealCancer; Hypothalamic and Visual Pathway Glioma, Childhood; IntraocularMelanoma; Islet Cell Carcinoma (Endocrine Pancreas); Kaposi's Sarcoma;Kidney Cancer; Laryngeal Cancer; Laryngeal Cancer, Childhood; Leukemia,Acute Lymphoblastic, Adult; Leukemia, Acute Lymphoblastic, Childhood;Leukemia, Acute Myeloid, Adult; Leukemia, Acute Myeloid, Childhood;Leukemia, Chronic Lyinphocytic; Leukemia, Chronic Myelogenous; Leukemia,Hairy Cell; Lip and Oral Cavity Cancer; Liver Cancer, Adult (Primary);Liver Cancer, Childhood (Primary); Lung Cancer, Non-Small Cell; LungCancer, Small Cell; Lymphoblastic Leukemia, Adult Acute; LymphoblasticLeukemia, Childhood Acute; Lymphocytic Leukemia, Chronic; Lymphoma,AIDS-Related; Lymphoma, Central Nervous System (Primary); Lymphoma,Cutaneous T-Cell: Lymphoma, Hodgkin's, Adult; Lymphoma, Hodgkin's,Childhood; Lymphoma, Hodgkin's During Pregnancy; Lymphoma,Non-Hodgkin's, Adult; Lymphoma, Non-Hodgkin's, Childhood; Lymphoma,Non-Hodgkin's During Pregnancy; Lymphoma, Primary Central NervousSystem; Macroglobulinemia, Waldenstrom's; Male Breast Cancer; MalignantMesothelioma, Adult; Malignant Mesothelioma, Childhood; MalignantThymoma; Medulloblastoma, Childhood; Melanoma; Melanoma, Intraocular;Merkel Cell Carcinoma; Mesothelioma, Malignant; Metastatic Squamous NeckCancer with Occult Primary; Multiple Endocrine Neoplasia Syndrome,Childhood; Multiple Myeloma/Plasma Cell Neoplasm; Mycosis Fungoides;Myelodysplastic Syndromes; Myelogenous Leukemia, Chronic; MyeloidLeukemia, Childhood Acute; Myeloma, Multiple; MyeloproliferativeDisorders, Chronic; Nasal Cavity and Paranasal Sinus Cancer;Nasopharyngeal Cancer; Nasopharyngeal Cancer, Childhood; Neuroblastoma;Non-Hodgkin's Lymphoma, Adult; Non-Hodgkin's Lymphoma, Childhood;Non-Hodgkins Lymphoma During Pregnancy; Non-Small Cell Lung Cancer; OralCancer, Childhood; Oral Cavity and Lip Cancer; Oropharyngeal Cancer;Osteosarcoma/Malignant Fibrous Histiocytoma of Bone; Ovarian Cancer,Childhood; Ovarian Epithelial Cancer; Ovarian Germ Cell Tumor; OvarianLow Malignant Potential Tumor; Pancreatic Cancer; Pancreatic Cancer,Childhood; Pancreatic Cancer, Islet Cell; Paranasal Sinus and NasalCavity Cancer; Parathyroid Cancer; Penile Cancer; Pheochromocytoma;Pineal and Supratentorial Primitive Neuroectodermal Tumors, Childhood;Pituitary Tumor; Plasma Cell Neoplasm/Multiple Myeloma; PleuroptilmonaryBlastoma; Pregnancy and Breast Cancer; Pregnancy and Hodgkin's Lymphoma;Pregnancy and Non-Hodgkin's Lymphoma; Primary Central Nervous SystemLymphoma; Primary Liver Cancer, Adult; Primary Liver Cancer, Childhood;Prostate Cancer; Rectal Cancer; Renal Cell (Kidney) Cancer; Renal CellCancer, Childhood; Renal Pelvis and Ureter, Transitional Cell Cancer;Retinoblastoma; Rhabdomyosarcoma, Childhood; Salivary Gland Cancer;Salivary Gland Cancer, Childhood; Sarcoma, Ewing's Family of Tumors;Sarcoma, Kaposi's; Sarcoma (Osteosarcoma)/Malignant Fibrous Histiocytomaof Bone; Sarcoma, Rhabdomyosarcoma, Childhood; Sarcoma, Soft Tissue,Adult; Sarcoma, Soft Tissue, Childhood; Sezary Syndrome; Skin Cancer;Skin Cancer, Childhood; Skin Cancer (Melanoma); Skin Carcinoma, MerkelCell; Small Cell Lung Cancer; Small Intestine Cancer; Soft TissueSarcoma, Adult; Soft Tissue Sarcoma, Childhood; Squamous Neck Cancerwith Occult Primary, Metastatic; Stomach (Gastric) Cancer; Stomach(Gastric) Cancer, Childhood; Supratentorial Primitive NeuroectodermalTumors, Childhood; T-Cell Lymphoma, Cutaneous; Testicular Cancer;Thymoma, Childhood; Thymoma, Malignant; Thyroid Cancer; Thyroid Cancer,Childhood; Transitional Cell Cancer of the Renal Pelvis and Ureter;Trophoblastic Tumor, Gestational; Unknown Primary Site, Cancer of,Childhood; Unusual Cancers of Childhood; Ureter and Renal Pelvis,Transitional Cell Cancer; Urethral Cancer; Uterine Sarcoma; VaginalCancer; Visual Pathway and Hypothalamic Glioma, Childhood; VulvarCancer; Waldenstrom's Macroglobulinemia; and Wilms' Tumor.

Further exemplary cancers include diffuse large B-cell lymphoma (DLBCL)and mantle cell lymphoma (MCL), Yet further exemplary cancers includeendocervical cancer, B-cell ALL, T-cell ALL, B- or T-cell lymphoma, mastcell cancer, glioblastoma, neuroblastoma, follicular lymphoma andRichter's syndrome.

Exemplary sarcomas include fibrosarcoma, alveolar soft part sarcoma(ASPS), liposarcoma, leiomyosarcoma, chondrosarcoma, synovial sarcoma,chordoma, spindle cell sarcoma, histiocytoma, rhabdomyosarcoma, Ewing'ssarcoma, neuroectodermal sarcoma, phyllodes/osteogenic sarcoma andchondroblastic osteosarcoma.

Metastases of the aforementioned cancers can also be treated orprevented in accordance with the methods described herein.

Combination Therapies

In some embodiments, the compound of Structural Formula I or (single)crystalline form thereof (e.g., in a pharmaceutical compositiondescribed herein) is administered together with an additional “second”therapeutic agent or treatment. The choice of second therapeutic agentmay be made from any agent that is typically used in a monotherapy totreat the indicated disease or condition. As used herein, the term“administered together” and related terms refers to the simultaneous orsequential administration of therapeutic agents in accordance with thisinvention. For example, the compound of Structural Formula I may beadministered with another therapeutic agent simultaneously orsequentially in separate unit dosage forms or together in a single unitdosage form. Accordingly, the present invention provides a single unitdosage form comprising the compound of Structural Formula I (e.g., acrystalline form or single crystalline form of the compound ofStructural Formula I), an additional therapeutic agent, and apharmaceutically acceptable carrier.

In one embodiment of the invention, where a second therapeutic agent isadministered to a subject, the effective amount of the compound ofStructural Formula I is less than its effective amount would be wherethe second therapeutic agent is not administered. In another embodiment,the effective amount of the second therapeutic agent is less than itseffective amount would be were the compound of Structural Formula I notadministered. In this way, undesired side effects associated with highdoses of either agent may be minimized. Other potential advantages(including without limitation improved dosing regimens and/or reduceddrug cost) will be apparent to those of skill in the art. The additionalagents may be administered separately, as part of a multiple doseregimen, from the compound of Structural Formula I. Alternatively, thoseagents may be part of a single dosage form, mixed together with thecompound of Structural Formula I in a single pharmaceutical composition.

In certain embodiments, the compound of Structural Formula I or (single)crystalline form thereof (e.g., in a pharmaceutical compositiondescribed herein) may be administered alone or in combination with othercompounds useful for treating or preventing inflammation. Exemplaryanti-inflammatory agents include, for example, steroids (e.g., Cortisol,cortisone, fludrocortisone, prednisone, 6[alpha]-methylprednisone,triamcinolone, betamethasone or dexamethasone), nonsteroidalantiinflammatory drugs (NSAIDS (e.g., aspirin, acetaminophen, tolmetin,ibuprofen, mefenamic acid, piroxicam, nabumetone, rofecoxib, celecoxib,etodolac or nimesulide). In another embodiment, the other therapeuticagent is an antibiotic (e.g., vancomycin, penicillin, amoxicillin,cefotaxime, ceftriaxone, cefixime, rifampinmetronidazole, doxycycline orstreptomycin). In another embodiment, the other therapeutic agent is aPDE4 inhibitor (e.g., roflumilast or rolipram). In another embodiment,the other therapeutic agent is an antihistamine (e.g., cyclizine,hydroxyzine, promethazine or diphenhydramine). In another embodiment,the other therapeutic agent is an anti-malarial (e.g., artemisinin,artemether, artsunate, chloroquine phosphate, mefloquine hydrochloride,doxycycline hyclate, proeuanil hydrochloride, atovaquone orhalofantrine). In one embodiment, the other compound is drotrecoginalfa. In a specific embodiment, the compound of Structural Formula I or(single) crystalline form thereof (e.g., in a pharmaceutical compositiondescribed herein) is administered in combination with dexamethasone.

Further examples of anti-inflammatory agents include, for example,aceclofenac, acemetacin, e-acetamidocaproic acid, acetaminophen,acetaminosalol, acetanilide, acetylsalicylic acid, S-adenosylmethionine,alclofenac, alclometasone, alfentanil, algestone, allylprodine,alminoprofen, aloxiprin, alphaprodine, aluminum bis(acetylsalicylate),amcinonide, amfenac, aminochlorthenoxazin, 3-amino-4-hydroxybutyricacid, 2-amino-4-picoline, aminopropylon, aminopyrine, amixetrine,ammonium salicylate, ampiroxicam, amtolmetin guacil, anileridine,antipyrine, antrafenine, apazone, beclomethasone, bendazac, benorylate,benoxaprofen, benzpiperylon, benzydamine, benzylmorphine, bermoprofen,betamethasone, betamethasone-17-valerate, bezitramide,[alpha]-bisabolol, bromfenac, p-bromoacetanilide, 5-bromosalicylic acidacetate, bromosaligenin, bucetin, bucioxic acid, bucolome, budesonide,bufexamac, bumadizon, buprenorphine, butacetin, butibufen, butorphanol,carbamazepine, carbiphene, caiprofen, carsalani, chlorohutanol,chloroprednisone, chlorthenoxazin, choline salicylate, cinchophen,cinmetacin, ciramadol, clidanac, clobetasol, clocortolone, clometacin,clonitazene, clonixin, clopirac, cloprednol, clove, codeine, codeinemethyl bromide, codeine phosphate, codeine sulfate, cortisone,cortivazol, cropropamide, crotethamide, cyclazocine, deflazacort,dehydrotestosterone, desomorphine, desonide, desoximetasone,dexamethasone, dexamethasone-21-isonicotinate, dexoxadrol,dextromoramide, dextropropoxyphene, deoxycorticosterone, dezocine,diampromide, diumorphone, diclofenac, difenamizole, difenpiramide,diflorasone, diflucortolone, diflunisal, difluprednate, dihydrocodeine,dihydrocodeinone enol acetate, dihydromorphine, dihydroxyaluminumacetylsalicylate, dimenoxadol, dimepheptanol, dimethylthiambutene,dioxaphetyl butyrate, dipipanone, diprocetyl, dipyrone, ditazol,droxicam, emorfazone, enfenamic acid, enoxolone, epirizole, eptazocine,etersalate, ethenzamide, ethoheptazine, ethoxazene,ethylmethylthiambutene, ethylmorphine, etodolac, etofenamate,etonitazene, eugenol, felbinac, fenbufen, fenclozic acid, fendosal,fenoprofen, fentanyl, fentiazac, fepradinol, feprazone, floctafenine,fluazacort, flucloronide, flufenamic acid, flumethasone, flunisolide,flunixin, flunoxaprofen, fluocinolone acetonide, fluocinonide,fluocinolone acetonide, fluocortin butyl, fluocoitolone, fluoresone,fluorometholone, fluperolone, flupirtine, fluprednidene,fluprednisolone, fluproquazone, flurandrenolide, flurbiprofen,fluticasone, formocortal, fosfosal, gentisic acid, glafenine,glucametacin, glycol salicylate, guaiazulene, halcinonide, halobetasol,halometasone, haloprednone, heroin, hydrocodone, hydro cortamate,hydrocortisone, hydrocortisone acetate, hydrocortisone succinate,hydrocortisone hemisuccinate, hydrocortisone 21-lysinate, hydrocortisonecypionate, hydromorphone, hydroxypethidine, ibufenac, ibuprofen,ibuproxam, imidazole salicylate, indomethacin, indoprofen, isofezolac,isoflupredone, isoflupredone acetate, isoladol, isomethadone, isonixin,isoxepac, isoxicam, ketobemidone, ketoprofen, ketorolac,p-lactophenetide, lefetamine, levallorphan, levorphanol,levophenacyl-morphan, lofentanil, lonazolac, lomoxicam, loxoprofen,lysine acetylsalicylate, mazipredone, meclofenamic acid, medrysone,mefenamic acid, meloxicam, meperidine, meprednisone, meptazinol,mesalamine, metazocine, methadone, methotrimeprazine,methylprednisolone, methylprednisolone acetate, methylprednisolonesodium succinate, methylprednisolone suleptnate, metiazinic acid,metofoline, metopon, mofebutazone, mofezolac, mometasone, morazone,morphine, morphine hydrochloride, morphine sulfate, morpholinesalicylate, myrophine, nabumetone, nalbuphine, nalorphine, 1-naphthylsalicylate, naproxen, narceine, nefopam, nicomorphine, nifenazone,niflumic acid, nimesulide, 5′-nitro-2′-propoxyacetanilide,norlevorphanol, normethadone, normorphine, norpipanone, olsalazine,opium, oxaceprol, oxametacine, oxaprozin, oxycodone, oxymorphone,oxyphenbutazone, papaveretum, paramethasone, paranyline, parsalmide,pentazocine, perisoxal, phenacetin, phenadoxone, phenazocine,phenazopyridine hydrochloride, phenocoll, phenoperidine, phenopyrazone,phenomorphan, phenyl acetylsalicylate, phenylbutazone, phenylsalicylate, phenyramidol, piketoprofen, piminodine, pipebuzone,piperylone, pirazolac, piritramide, piroxicam, pirprofen, pranoprofen,prednicarbate, prednisolone, prednisone, prednival, prednylidene,proglumetacin, proheptazine, promedol, propacetamol, properidine,propiram, propoxyphene, propyphenazone, proquazone, protizinic acid,proxazole, ramifenazone, remifentanil, rimazolium metilsulfate,salacetamide, salicin, salicylamide, salicylamide o-acetic acid,salicylic acid, salicylsulfuric acid, salsalate, salverine, sametride,sufentanil, sulfasalazine, sulindac, superoxide dismutase, suprofen,suxibuzone, talniflumate, tenidap, tenoxicam, terofenamate, tetrandrine,thiazolinobutazone, tiaprofenic acid, tiaramide, tilidine, tinoridine,tixocortol, tolfenamic acid, tolmetin, tramadol, triamcinolone,triamcinolone, acetonide, tropesin, viminol, xenbucin, ximoprofen,zaltoprofen and zomepirac.

In one embodiments, the compound of Structural Formula I may beadministered with a selective COX-2 inhibitor for treating or preventinginflammation. Exemplary selective COX-2 inhibitors include, for example,deracoxib, parecoxib, celecoxib, valdecoxib, rofecoxib, etoricoxib, andlumiracoxib.

In some embodiments, the compound of Structural Formula I isadministered in combination with an anthracycline or a Topo IIinhibitor. In certain embodiments, the compound of Structural Formula Iis administered in combination with Doxorubicin (Dox). In certainembodiments, the compound of Structural Formula I is administered incombination with bortezomib (and more broadly including carfilzomib).

Cancer Combination Therapies

In some embodiments, the compound of Structural Formula I or (single)crystalline form thereof, (e.g., in a pharmaceutical compositiondescribed herein) is administered together with an additional cancertreatment. Exemplary additional cancer treatments include, for example:chemotherapy, targeted therapies such as antibody therapies, kinaseinhibitors, immunotherapy, and hormonal therapy, epigenetic therapy,proteosome inhibitors, and anti-angiogenic therapies. Examples of eachof these treatments are provided below. As used herein, the term“combination,” “combined,” and related terms refer to the simultaneousor sequential administration of therapeutic agents in accordance withthis invention. For example, the compound of Structural Formula I can beadministered with another therapeutic agent simultaneously orsequentially in separate unit dosage forms or together in a single unitdosage form. Accordingly, the present invention provides a single unitdosage form comprising the compound of Structural Formula I (e.g., acrystalline form or single crystalline form of the compound ofStructural Formula I), an additional therapeutic agent, and apharmaceutically acceptable carrier.

The amount of both the compound of Structural Formula I and additionaltherapeutic agent (in those pharmaceutical compositions which comprisean additional therapeutic agent as described above) that can be combinedwith the carrier materials to produce a single dosage form will varydepending upon the host treated and the particular mode ofadministration. Preferably, pharmaceutical compositions of thisinvention should be formulated so that a dosage of between 0.01-100mg/kg body weight/day of a compound of Structural Formula I can beadministered.

Chemotherapy

In some embodiments, a pharmaceutical composition described herein isco-administered with a chemotherapy. Chemotherapy is the treatment ofcancer with drugs that can destroy cancer cells. “Chemotherapy” usuallyrefers to cytotoxic drugs which affect rapidly dividing cells ingeneral, in contrast with targeted therapy. Chemotherapy drugs interferewith cell division in various possible ways, e.g., with the duplicationof DNA or the separation of newly formed chromosomes. Most forms ofchemotherapy target all rapidly dividing cells and are not specific forcancer cells, although some degree of specificity may come from theinability of many cancer cells to repair DNA damage, while normal cellsgenerally can.

Examples of chemotherapeutic agents used in cancer therapy include, forexample, antimetabolites (e.g., folic acid, purine, and pyrimidinederivatives) and alkylating agents (e.g., nitrogen mustards,nitrosoureas, platinum, alkyl sulfonates, hydrazines, triazenes,aziridines, spindle poison, cytotoxic agents, topoisomerase inhibitorsand others). Exemplary agents include Aclarubicin, Actinomycin,Alitretinoin, Altretamine, Aminopterin, Aminolevulinic acid, Amrubicin,Amsacrine, Anagrelide, Arsenic trioxide, Asparaginase, Atrasentan,Belotecan, Bexarotene, Bendamustine, Bleomycin, Bortezomib, Busulfan,Camptothecin, Capecitabine, Carboplatin, Carboquone, Carfilzomib,Carmofur, Carmustine, Celecoxib, Cetuximab, Chlorambucil, Chlormethine,CHOEP-21, CHOP, Cisplatin, Cladribine, Clofarabine, Crisantaspase,Cyclophosphamide, Cytarabine or ara-C, Dacarbazine, Dactinomycin, DAEPOCH, Daratumumab, Daunorubicin, Decitabine, Demecolcine,Dexamethasone, Docetaxel, Doxorubicin, Efaproxiral, Elesclomol,Elsamitrucin, Enocitabine, Epirubicin, Eribulin, Estramustine,Etoglucid, Etoposide, FLAG (Flu+Cyt), Floxuridine, Fludarabine,Fluorouracil (5FU), FOLFOX, Fotemustine, Gemcitabine,gemcitabine-oxaliplatin. (GemOx), Gliadel implants, Hydroxycarbamide,Hydroxyurea, Ibrutinib, Idarubicin, Ifosfamide, Irinotecan, Irofulven,Ixabepilone, Ixazomib, Larotaxel, Lenalidomide, Leucovorin, Liposomaldoxorubicin, Liposomal daunorubicin, Lonidamine, Lomustine, Lucanthone,Mannosulfan, Masoprocol, Melphalan, Mercaptopurine, Mesna, Methotrexate,Methyl aminolevulinate, Mitobronitol, Mitoguazone, Mitotane, Mitomycin,Mitoxantrone, Nab-paclitaxel, Nedaplatin, Nimustine, Oblimersen,Onacetaxine, Ortataxel, Oxahplatin, Paclitaxel, Pegaspargase,Pemetrexed, Pentostatin, Pirarubicin, Pixantrone, PLD (pegylatedliposomal doxorubicin), Plicamycin, Pomalidomide, Porfimer sodium,Prednimustine, Procarbazine, Raltitrexed, Ranimustine, R-CHOP, r-dhaox,r-dhap, Rituximab, Romidepsin, Rubitecan, Sapacitabine, Semustine,Sitimagene ceradenovec, Sorafonib, Strataplatin, Streptozocin,Talaporfin, Tegafur-uracil, Temoporfin, Temozolomide, Teniposide,Tesetaxel, Testolactone, Tetranitrate, Thiotepa, Tiazofurine,Tioguanine, Tipifarnib, Topotecan, Trabectedin, Triaziquone,Triethylenemelamine, Triplatin, Tretinoin, Treosulfan, Trofosfamide,Uramustine, Valrubicin, Verteporfin, Vinblastine, Vincristine,Vindesine, Vinflunine, Vinorelbine, Vorinostat, Zorubicin, and othercytostatic or cytotoxic agents described herein.

Because some drugs work better together than alone, two or more drugsare often given at the same time. Often, two or more chemotherapy agentsare used as combination chemotherapy. In some embodiments, thechemotherapy agents (including combination chemotherapy) can be used incombination with a pharmaceutical composition described herein.

Targeted Therapy

Targeted therapy constitutes the use of agents specific for thederegulated proteins of cancer cells. Small molecule targeted therapydrugs are generally inhibitors of enzymatic domains on mutated,overexpressed, or otherwise critical proteins within the cancer cell.Prominent examples are the tyrosine kinase inhibitors such as Axitinib,Bosutinib, Cediranib, desatinib, erolotinib, imatinib, gefitinib,lapatinib, Lestaurtinib, Nilotinib, Semaxanib, Sorafenib, Sunitinib, andVandetanib, and also cyclin-dependent kinase inhibitors such asAlvocidib and Seliciclib. Monoclonal antibody therapy is anotherstrategy in which the therapeutic agent is an antibody whichspecifically binds to a protein on the surface of the cancer cells.Examples include the anti-HER2/neu antibody trastuzumab (Herceptin®)typically used in breast cancer, and the anti-CD20 antibody rituximaband Tositumomab typically used in a variety of B-cell malignancies.Other exemplary antibodies include Cetuximab, Panitumumab, Trastuzumab,Alemtuzumab, Bevacizumab, Edrecolomab, and Gemtuzumab. Exemplary fusionproteins include Aflibercept and Denileukin diftitox. In someembodiments, the targeted therapy can be used in combination with apharmaceutical composition described herein, e.g., Gleevec (Vignari andWang 2001).

Targeted therapy can also involve small peptides as “homing devices”which can bind to cell surface receptors or affected extracellularmatrix surrounding the tumor. Radionuclides which are attached to thesepeptides (e.g., RGDs) eventually kill the cancer cell if the nuclidedecays in the vicinity of the cell. An example of such therapy includesBEXXAR®.

Angiogenesis

Pharmaceutical compositions described herein may be used to treat orprevent a disease or disorder associated with angiogenesis. Diseasesassociated with angiogenesis include cancer, cardiovascular disease andmacular degeneration.

Angiogenesis is the physiological process involving the growth of newblood vessels from pre-existing vessels. Angiogenesis is a normal andvital process in growth and development, as well as in wound healing andin granulation tissue. However, it is also a fundamental step in thetransition of tumors from a dormant state to a malignant one.Angiogenesis may be a target for combating diseases characterized byeither poor vascularization or abnormal vasculature.

Application of specific compounds that may inhibit or induce thecreation of new blood vessels in the body may help combat such diseases.The presence of blood vessels where there should be none may affect themechanical properties of a tissue, increasing the likelihood of failure.The absence of blood vessels in a repairing or otherwise metabolicallyactive tissue may inhibit repair or other essential functions. Severaldiseases, such as ischemic chronic wounds, are the result of failure orinsufficient blood vessel formation and may be treated by a localexpansion of blood vessels, thus bringing new nutrients to the site,facilitating repair. Other diseases, such as age-related maculardegeneration, may be created by a local expansion of blood vessels,interfering with normal physiological processes.

Vascular endothelial growth factor (VEGF) has been demonstrated to be amajor contributor to angiogenesis, increasing the number of capillariesin a given network. Upregulation of VEGF is a major component of thephysiological response to exercise and its role in angiogenesis issuspected to be a possible treatment in vascular injuries. In vitrostudies clearly demonstrate that VEGF is a potent stimulator ofangiogenesis because, in the presence of this growth factor, platedendothelial cells will proliferate and migrate, eventually forming tubestructures resembling capillaries.

Tumors induce blood vessel growth (angiogenesis) by secreting variousgrowth factors (e.g., VEGF). Growth factors such as bFGF and VEGF caninduce capillary growth into the tumor, which some researchers suspectsupply required nutrients, allowing for tumor expansion.

Angiogenesis represents an excellent therapeutic target for thetreatment of cardiovascular disease. It is a potent, physiologicalprocess that underlies the natural manner in which our bodies respond toa diminution of blood supply to vital organs, namely the production ofnew collateral vessels to overcome the ischemic insult.

Overexpression of VEGF causes increased permeability in blood vessels inaddition to stimulating angiogenesis. In wet macular degeneration, VEGFcauses proliferation of capillaries into the retina. Since the increasein angiogenesis also causes edema, blood and other retinal fluids leakinto the retina, causing loss of vision.

Anti-angiogenic therapy can include kinase inhibitors targeting vascularendothelial growth factor (VEGF) such as sunitinib, sorafenib, ormonoclonal antibodies or receptor “decoys” to VEGF or VEGF receptorincluding bevacizumab or VEGF-Trap, or thalidomide or its analogs(lenalidomide, pomalidomide), or agents targeting non-VEGF angiogenictargets such as fibroblast growth factor (FGF), angiopoietins, orangiostatin or endostatin.

Epigenetics

Pharmaceutical compositions described herein may be used to treat orprevent a disease or disorder associated with epigenetics. Epigeneticsis the study of heritable changes in phenotype or gene expression causedby mechanisms other than changes in the underlying DNA sequence. Oneexample of epigenetic changes in eukaryotic biology is the process ofcellular differentiation. During morphogenesis, stem cells become thevarious cell lines of the embryo which in turn become fullydifferentiated cells. In other words, a single fertilized egg cellchanges into the many cell types including neurons, muscle cells,epithelium, blood vessels etc. as it continues to divide. It does so byactivating some genes while inhibiting others.

Epigenetic changes are preserved when cells divide. Most epigeneticchanges only occur within the course of one individual organism'slifetime, but, if a mutation in the DNA has been caused in sperm or eggcell that results in fertilization, then some epigenetic changes areinherited from one generation to the next. Specific epigenetic processesinclude paramutation, bookmarking, imprinting, gene silencing, Xchromosome inactivation, position effect, reprogramming, transvection,maternal effects, the progress of carcinogenesis, many effects ofteratogens regulation of historic modifications and heterochromatin, andtechnical limitations affecting parthenogenesis and cloning.

Exemplary diseases associated with epigenetics include ATR-syndrome,fragile X-syndrome, ICF syndrome, Angelman's syndrome, Prader-Willssyndrome, BWS, Rett syndrome, α-thalassaemia, cancer, leukemia,Rubinstein-Taybi syndrome and Coffin-Lowry syndrome.

The first human disease to be linked to epigenetics was cancer.Researcners found that diseased tissue from patients with colorectalcancer had less DNA methylation than normal tissue from the samepatients. Because methylated genes are typically turned off, loss of DNAmethylation can cause abnormally high gene activation by altering thearrangement of chromatin. On the other hand, too much methylation canundo the work of protective tumor suppressor genes.

DNA methylation occurs at CpG sites, and a majority of CpG cytosines aremethylated in mammals. However, there are stretches of DNA near promoterregions that have higher concentrations of CpG sites (known as CpGislands) that are free of methylation in normal cells. These CpG islandsbecome excessively methylated in cancer cells, thereby causing genesthat should not be silenced to turn off. This abnormality is thetrademark epigenetic change that occurs in tumors and happens early inthe development of cancer. Hypermethylation of CpG islands can causetumors by shutting off tumor-suppressor genes. In fact, these types ofchanges may be more common in human cancer than DNA sequence mutations.

Furthermore, although epigenetic changes do not alter the sequence ofDNA, they can cause mutations. About half of the genes that causefamilial or inherited forms of cancer are turned off by methylation.Most of these genes normally suppress tumor formation and help repairDNA, including O6-methylguanine-DNA methyltransferase (MGMT), MLH1cyclin-dependent kinase inhibitor 2B (CDKN2B), and RASSF1A. For example,hypermethylation of the promoter of MGMT causes the number of G-to-Amutations to increase.

Hypermethylation can also lead to instability of microsatellites, whichare repeated sequences of DNA. Microsatellites are common in normalindividuals, and they usually consist of repeats of the dinucleotide CA.Too much methylation of the promoter of the DNA repair gene MLH1 canmake a microsatellite unstable and lengthen or shorten it.Microsatellite instability has been linked to many cancers, includingcolorectal, endometrial, ovarian, and gastric cancers.

Fragile X syndrome is the most frequently inherited mental disability,particularly in males. Both sexes can be affected by this condition, butbecause males only have one X chromosome, one fragile X will impact themmore severely. Indeed, fragile X syndrome occurs in approximately 1 in4,000 males and 1 in 8,000 females. People with this syndrome havesevere intellectual disabilities, delayed verbal development, and“autistic-like” behavior.

Fragile X syndrome gets its name from the way the part of the Xchromosome that contains the gene abnormality looks under a microscope;it usually appears as if it is hanging by a thread and easily breakable.The syndrome is caused by an abnormality in the FMR1 (fragile X mentalretardation 1) gene. People who do not have fragile X syndrome have 6 to50 repeats of the trinucleotide CCG in their FMR1 gene. However,individuals with over 200 repeats have a full mutation, and they usuallyshow symptoms of the syndrome. Too many CGGs cause the CpG islands atthe promoter region of the FMR1 gene to become methylated; normally,they are not. This methylation turns the gene off, stopping the FMR1gene from producing an important protein called fragile X mentalretardation protein. Loss of this specific protein causes fragile Xsyndrome. Although a lot of attention has been given to the CCGexpansion mutation as the cause of fragile X, the epigenetic changeassociated with FMR1 methylation is the real syndrome culprit.

Fragile X syndrome is not the only disorder associated with mentalretardation that involves epigenetic changes. Other such conditionsinclude Rubenstein-Taybi, Coffin-Lowry, Prader-Willi, Angelman,Beckwith-Wiedemann, ATR-X, and Rett syndromes.

Epigenetic therapies include inhibitors of enzymes controllingepigenetic modifications, specifically DNA methyltransferases andhistone deacetylases, which have shown promising anti-tumorigeniceffects for some malignancies, as well as antisense oligonucleotides andsiRNA.

Immunotherapy

In some embodiments, a pharmaceutical composition described herein isadministered with an immunotherapy. Cancer immunotherapy refers to adiverse set of therapeutic strategies designed to induce the patient'sown immune system to fight the tumor. Contemporary methods forgenerating an immune response against tumors include intravesicular BCGimmunotherapy for superficial bladder cancer, prostate cancer vaccineProvenge, and use of interferons and other cytokines to induce an immuneresponse in renal cell carcinoma and melanoma patients.

Allogeneic hematopoietic stem cell transplantation can be considered aform of immunotherapy, since the donor's immune cells will often attackthe tumor in a graft-versus-tumor effect. In some embodiments, theimmunotherapy agent(s) can be used in combination with a pharmaceuticalcomposition described herein.

Hormonal Therapy

In some embodiments, a pharmaceutical composition described herein isadministered with a hormonal therapy. The growth of some cancers can beinhibited by providing or blocking certain hormones. Common examples ofhormone-sensitive tumors include certain types of breast and prostatecancers, as well as certain types of leukemia which respond to certainretinoids/retinoic acids. Removing or blocking estrogen or testosteroneis often an important additional treatment. In certain cancers,administration of hormone agonists, such as progestogens may betherapeutically beneficial. In some embodiments, the hormonal therapyagents can be used in combination with a pharmaceutical compositiondescribed herein.

Hormonal therapy agents include the administration of hormone agonistsor hormone antagonists and include retinoids/retinoic acid, compoundsthat inhibit estrogen or testosterone, as well as administration ofprogestogens.

Inflammation and Autoimmune Disease

The pharmaceutical compositions described herein may be used to treat orprevent a disease or disorder associated with inflammation, particularlyin humans and other mammals. A pharmaceutical composition describedherein may be administered prior to the onset of, at, or after theinitiation of inflammation. When used prophylactically, thepharmaceutical compositions are preferably provided in advance of anyinflammatory response or symptom. Administration of the pharmaceuticalcompositions can prevent or attenuate inflammatory responses orsymptoms. Exemplary inflammatory conditions include, for example,multiple sclerosis, rheumatoid arthritis, psoriatic arthritis,degenerative joint disease, spondouloarthropathies, other seronegativeinflammatory arthridities, polymyalgia rheumatica, various vasculidities(e.g., giant cell arteritis, ANCA+ vasculitis), gouty arthritis,systemic lupus erythematosus, juvenile arthritis, juvenile rheumatoidarthritis, osteoarthritis, osteoporosis, diabetes (e.g., insulindependent diabetes mellitus or juvenile onset diabetes), menstrualcramps, cystic fibrosis, inflammatory bowel disease, irritable bowelsyndrome, Crohn's disease, mucous colitis, ulcerative colitis,gastritis, esophagitis, pancreatitis, peritonitis, Alzheimer's disease,shock, ankylosing spondylitis, gastritis, conjunctivitis, pancreatis(acute or chronic), multiple organ injury syndrome (e.g., secondary tosepticemia or trauma), myocardial infarction, atherosclerosis, stroke,reperfusion injury (e.g., due to cardiopulmonary bypass or kidneydialysis), acute glomerulonephritis, thermal injury (i.e., sunburn),necrotizing enterocolitis, granulocyte transfusion associated syndrome,and/or Sjogren's syndrome. Exemplary inflammatory conditions of the skininclude, for example, eczema, atopic dermatitis, contact dermatitis,urticaria, schleroderma, psoriasis, and dermatosis with acuteinflammatory components.

In another embodiment, a pharmaceutical composition described herein maybe used to treat or prevent allergies and respiratory conditions,including asthma, bronchitis, pulmonary fibrosis, allergic rhinitis,oxygen toxicity, emphysema, chronic bronchitis, acute respiratorydistress syndrome, and any chronic obstructive pulmonary disease (COPD).The compounds may be used to treat chronic hepatitis infection,including hepatitis B and hepatitis C.

Additionally, a pharmaceutical composition described herein may be usedto treat autoimmune diseases and/or inflammation associated withautoimmune diseases, such as organ-tissue autoimmune diseases (e.g.,Raynaud's syndrome), scleroderma, myasthenia gravis, transplantrejection, endotoxin shock, sepsis, psoriasis, eczema, dermatitis,multiple sclerosis, autoimmune thyroiditis, uveitis, systemic lupuserythematosis, Addison's disease, autoimmune polyglandular disease (alsoknown as autoimmune polyglandular syndrome), and Grave's disease.

In a particular embodiment, the pharmaceutical compositions describedherein can be used to treat multiple sclerosis.

Viral Infections

Pharmaceutical compositions described herein may be used to treat orprevent a disease or disorder associated with a viral infection,particularly in humans and other mammals. A pharmaceutical compositiondescribed herein may be administered prior to the onset of, at, or afterthe initiation of viral infection. When used prophylactically, thepharmaceutical composition is preferably provided in advance of anyviral infection or symptom thereof.

Exemplary viral diseases include acute febrile pharyngitis,pharyngoconjunctival fever, epidemic keratoconjunctivitis, infantilegastroenteritis, Coxsackie infections, infectious mononucleosis, Burkittlymphoma, acute hepatitis, chronic hepatitis, hepatic cirrhosis,hepatocellular carcinoma, primary HSV-1 infection (e.g.,gingivostomatitis in children, tonsillitis and pharyngitis in adults,keratoconjunctivitis), latent HSV-1 infection (e.g., herpes labialis andcold sores), primary HSV-2 infection, latent HSV-2 infection, asepticmeningitis, infectious mononucleosis, Cytomegalic inclusion disease,Kaposi's sarcoma, multicentric Castleman disease, primary effusionlymphoma, AIDS, influenza, Reye syndrome, measles, postinfectiousencephalomyelitis, Mumps, hyperplastic epithelial lesions (e.g., common,flat, plantar and anogenital warts, laryngeal papillomas,epidermodysplasia verruciformis), cervical carcinoma, squamous cellcarcinomas, croup, pneumonia, bronchiolitis, common cold, Poliomyelitis,Rabies, influenza-like syndrome, severe bronchiolitis with pneumonia,German measles, congenital rubella, Varicella, and herpes zoster.

Exemplary viral influenza A strains include H1N1, H3N2, H5N1, H7N3,H7N9. A compound described herein can also be used to treat or preventinfluenza B.

Exemplary viral pathogens include Adenovirus, Coxsackievirus, Denguevirus, Encephalitis Virus, Epstein-Barr virus, Hepatitis A virus,Hepatitis B virus, Hepatitis C virus, Herpes simplex virus type I,Herpes simplex virus type 2, cytomegalovirus, Human herpesvirus type 8,Human immunodeficiency virus, Influenza virus, measles virus, Mumpsvirus, Human papillomavirus, Parainfluenza virus, Poliovirus, Rabiesvirus, Respiratory syncytial virus, Rubella virus, Varicella-zostervirus, West Nile virus, Dungee, and Yellow fever virus. Viral pathogensmay also include viruses that cause resistant viral infections.

Antiviral drugs are a class of medications used specifically fortreating viral infections. Antiviral action generally falls into one ofthree mechanisms: interference with the ability of a virus to infiltratea target cell (e.g., amantadine, rimantadine and pleconaril), inhibitionof the synthesis of virus (e.g., nucleoside analogues, e.g., acyclovirand zidovudine (AZT), and inhibition of the release of virus (e.g.,zanamivir and oseltamivir).

Ophthalmology

Pharmaceutical compositions described herein may be used to treat orprevent ophthamology disorder. Exemplary ophthamology disorders includemacular edema (diabetic and nondiabetic macular edema), age relatedmacular degeneration wet and dry forms, aged disciform maculardegeneration, cystoid macular edema, palpebral edema, retina edema,diabetic retinopathy, chorioretinopathy, neovascular maculopathy,neovascular glaucoma, uveitis, iritis, retinal vasculitis,endophthalmitis, panophthalmitis, metastatic ophthalmia, choroiditis,retinal pigment epithelitis, conjunctivitis, cyclitis, scleritis,episcleritis, optic neuritis, retrobulbar optic neuritis, keratitis,blepharitis, exudative retinal detachment, corneal ulcer, conjunctivalulcer, chronic nummular keratitis, ophthalmic disease associated withhypoxia or ischemia, retinopathy of prematurity, proliferative diabeticretinopathy, polypoidal choroidal vasculopathy, retinal angiomatousproliferation, retinal artery occlusion, retinal vein occlusion, Coats'disease, familial exudative vitreoretinopathy, pulseless disease(Takayasu's disease), Eales disease, antiphospholipid antibody syndrome,leukemic retinopathy, blood hyperviscosity syndrome, macroglobulinemia,interferon-associated retinopathy, hypertensive retinopathy, radiationretinopathy, corneal epithelial stem cell deficiency and cataract.

Other ophthalmology disorders treatable using the pharmaceuticalcompositions described herein include proliferative vitreoretinopathyand chronic retinal detachment.

Inflammatory eye diseases are also treatable using the pharmaceuticalcompositions described herein.

Neurodegenerative Disease

Pharmaceutical compositions described herein may be used to treat orprevent an neurodegenerative disease. Neurodegeneration is the umbrellaterm for the progressive loss of structure or function of neurons,including death of neurons. Many neurodegenerative diseases includingParkinson's, Alzheimer's, and Huntington's occur as a result ofneurodegenerative processes. As research progresses, many similaritiesappear which relate these diseases to one another on a sub-cellularlevel. Discovering these similarities offers hope for therapeuticadvances that could ameliorate many diseases simultaneously. There aremany parallels between different neurodegenerative disorders includingatypical protein assemblies as well as induced cell death.

Alzheimer's disease is characterized by loss of neurons and synapses inthe cerebral cortex and certain subcortical regions. This loss resultsin gross atrophy of the affected regions, including degeneration in thetemporal lobe and parietal lobe, and parts of the frontal cortex andcingulate gyrus.

Huntington's disease causes astrogliosis and loss of medium spinyneurons. Areas of the brain are affected according to their structureand the types of neurons they contain, reducing in size as theycumulatively lose cells. The areas affected are mainly in the striatum,but also the frontal and temporal cortices. The striatum's subthalamicnuclei send control signals to the globus pallidus, which initiates andmodulates motion. The weaker signals from subthalamic nuclei thus causereduced initiation and modulation of movement, resulting in thecharacteristic movements of the disorder. Exemplary treatments forHuntington's disease include tetrabenazine, neuroleptics,benzodiazepines, amantadine, remacemide, valproic acid, selectiveserotonin reuptake inhibitors (SSRIs), mirtazapine and antipsychotics.

The mechanism by which the brain cells in Parkinson's are lost mayconsist of an abnormal accumulation of the protein alpha-synuclein boundto ubiquitin in the damaged cells. The alpha-synuclein-ubiquitin complexcannot be directed to the proteosome. This protein accumulation formsproteinaceous cytoplasmic inclusions called Lewy bodies. The latestresearch on pathogenesis of disease has shown that the death ofdopaminergic neurons by alpha-synuclein is due to a defect in themachinery that transports proteins between two major cellular organellesthe endoplasmic reticulum (ER) and the Golgi apparatus. Certain proteinslike Rab1 may reverse this defect caused by alpha-synuclein in animalmodels. Exemplary Parkinson's disease therapies include levodopa,dopamine agonists such as include bromocriptine, pergolide, pramipexole,ropinirole, piribedil, cabergoline, apomorphine and lisuride, dopadecarboxylate inhibitors, MAO-B inhibitors such as selegilene andrasagilene, anticholinergics and amantadine.

Amyotrophic lateral sclerosis (ALS/Lou Gehrig's Disease is a disease inwhich motor neurons are selectively targeted for degeneration. ExemplaryALS therapies include riluzole, baclofen, diazepam, trihexyphenidyl andamitriptyline.

Other exemplary neurodegenerative therapeutics includes antisenseoligonucleotides and stem cells.

Wound Healing

Wounds are a type of condition characterized by cell or tissue damage.Wound healing is a dynamic pathway that optimally leads to restorationof tissue integrity and function. The wound healing process consists ofthree overlapping phases. The first phase is an inflammatory phase,which is characterized by homeostasis, platelet aggregation anddegranulation. Platelets as the first response, release multiple growthfactors to recruit immune cells, epithelial cells, and endothelialcells. The inflammatory phase typically occurs over days 0-5. The secondstage of wound healing is the proliferative phase during whichmacrophages and granulocytes invade the wound. Infiltrating fibroblastsbegin to produce collagen. The principle characteristics of this phaseare epithelialization, angiogenesis, granulation tissue formation andcollagen production. The proliferative phase typically occurs over days3-14. The third phase is the remodeling phase where matrix formationoccurs. The fibroblasts, epithelial cells, and endothelial cellscontinue to produce collagen and collagenase as well as matrixmetalloproteases (MMPs) for remodeling. Collagen crosslinking takesplace and the wound undergoes contraction. The remodeling phasetypically occurs from day 7 to one year.

Pharmaceutical compositions described herein can be used for promotingwound healing (e.g., promoting or accelerating wound closure and/orwound healing, mitigating scar fibrosis of the tissue of and/or aroundthe wound, inhibiting apoptosis of cells surrounding or proximate to thewound). Thus, in certain embodiments, the present invention provides amethod for promoting wound healing in a subject, comprisingadministering to the subject a therapeutically effective amount of apharmaceutical composition described herein. The method need not achievecomplete healing or closure of the wound; it is sufficient for themethod to promote any degree of wound closure. In this respect, themethod can be employed alone or as an adjunct to other methods forhealing wounded tissue.

Pharmaceutical compositions described herein can be used to treat woundsduring the inflammatory (or early) phase, during the proliferative (ormiddle) wound healing phase, and/or during the remodeling (or late)wound healing phase.

In some embodiments, the subject in need of wound healing is a human oran animal, for example, a dog, a cat, a horse, a pig, or a rodent, suchas a mouse.

In some embodiments, the pharmaceutical compositions described hereinuseful for wound healing are administered topically, for example,proximate to the wound site, or systemically.

More specifically, a therapeutically effective amount of apharmaceutical composition described herein can be administered(optionally in combination with other agents) to the wound site bycoating the wound or applying a bandage, packing material, stitches,etc., that are coated or treated with the compound or compositiondescribed herein. As such, the pharmaceutical compositions describedherein can be formulated for topical administration to treat surfacewounds. Topical formulations include those for delivery via the mouth(buccal) and to the skin such that a layer of skin (i.e., the epidermis,dermis, and/or subcutaneous layer) is contacted with the pharmaceuticalcomposition described herein. Topical delivery systems may be used toadminister topical formulations of the compounds and compositionsdescribed herein.

Alternatively, the pharmaceutical compositions described herein can beadministered at or near the wound site by, for example, injection of asolution, injection of an extended release formulation, or introductionof a biodegradable implant comprising the compound or compositiondescribed herein.

The pharmaceutical compositions described herein can be used to treatacute wounds or chronic wounds. A chronic wound results when the normalreparative process is interrupted. Chronic wounds can develop from acuteinjuries as a result of unrecognized persistent infections or inadequateprimary treatment. In most cases however, chronic lesions are the endstage of progressive tissue breakdown owing to venous, arterial, ormetabolic vascular disease, pressure sores, radiation damage, or tumors.

In chronic wounds, healing does not occur for a variety of reasons,including improper circulation in diabetic ulcers, significant necrosis,such as in burns, and infections. In these chronic wounds, viability orthe recovery phase is often the rate-limiting step. The cells are nolonger viable and, thus, initial recovery phase is prolonged byunfavorable wound bed environment.

Chronic wounds include, but are not limited to the following: chronicischemic skin lesions; scleroderma ulcers; arterial ulcers; diabeticfoot ulcers; pressure ulcers; venous ulcers; non-healing lower extremitywounds; ulcers due to inflammatory conditions; and/or long-standingwounds. Other examples of chronic wounds include chronic ulcers,diabetic wounds, wounds caused by diabetic neuropathy, venousinsufficiencies, and arterial insufficiencies, and pressure wounds andcold and warm burns. Yet other examples of chronic wounds includechronic ulcers, diabetic wounds, wounds caused by diabetic neuropathy,venous insufficiencies, arterial insufficiencies, and pressure wounds.

Acute wounds include, but are not limited to, post-surgical wounds,lacerations, hemorrhoids and fissures.

In a particular embodiment, the pharmaceutical compositions describedherein can be used for diabetic wound healing or accelerating healing ofleg and foot ulcers secondary to diabetes or ischemia in a subject.

In one embodiment, the wound is a surface wound. In another embodiment,the wound is a surgical wound (e.g., abdominal or gastrointestinalsurgical wound). In a further embodiment, the wound is a burn. In yetanother embodiment, the wound is the result of radiation exposure.

The pharmaceutical compositions described herein can also be used fordiabetic wound healing, gastrointestinal wound healing, or healing of anadhesion due, for example, to an operation.

The pharmaceutical compositions described herein can also be used toheal wounds that are secondary to another disease. For example, ininflammatory skin diseases, such as psoriasis and dermatitis, there arenumerous incidents of skin lesions that are secondary to the disease,and are caused by deep cracking of the skin, or scratching of the skin.The pharmaceutical compositions described herein can be used to healwounds that are secondary to these diseases, for example, inflammatoryskin diseases, such as psoriasis and dermatitis.

In a further embodiment, the wound is an internal wound. In a specificaspect, the internal wound is a chronic wound. In another specificaspect, the wound is a vascular wound. In yet another specific aspect,the internal wound is an ulcer. Examples of internal wounds include, butare not limited to, fistulas and internal wounds associated withcosmetic surgery, internal indications, Crohn's disease, ulcerativecolitis, internal surgical sutures and skeletal fixation. Other examplesof internal wounds include, but are not limited to, fistulas andinternal wounds associated with cosmetic surgery, internal indications,internal surgical sutures and skeletal fixation.

Examples of wounds include, but are not limited to, abrasions,avulsions, blowing wounds (i.e., open pneumothorax), burn wounds,contusions, gunshot wounds, incised wounds, open wounds, penetratingwounds, perforating wounds, puncture wounds, seton wounds, stab wounds,surgical wounds, subcutaneous wounds, diabetic lesions, or tangentialwounds. Additional examples of wounds that can be treated by thepharmaceutical compositions described herein include acute conditions orwounds, such as thermal burns, chemical burns, radiation burns, bumscaused by excess exposure to ultraviolet radiation sunburn); damage tobodily tissues, such as the perineum as a result of labor andchildbirth; injuries sustained during medical procedures, such asepisiotomies; trauma-induced injuries including cuts, incisions,excoriations; injuries sustained from accidents; post-surgical injuries,as well as chronic conditions, such as pressure sores, bedsores,conditions related to diabetes and poor circulation, and all types ofacne. In addition, the wound can include dermatitis, such as impetigo,intertrigo, folliculitis and eczema, wounds following dental surgery;periodontal disease; wounds following trauma; and tumor-associatedwounds. Yet other examples of wounds include animal bites, arterialdisease, insect stings and bites, bone infections, compromisedskin/muscle grafts, gangrene, skin tears or lacerations, skin aging,surgical incisions, including slow or non-healing surgical wounds,intracerebral hemorrhage, aneurysm, dermal asthenia, and post-operationinfections.

In preferred embodiments, the wound is selected from the groupconsisting of a burn wound, an incised wound, an open wound, a surgicalor post surgical wound, a diabetic lesion, a thermal burn, a chemicalburn, a radiation burn, a pressure sore, a bedsore, and a conditionrelated to diabetes or poor circulation. In more preferred embodiments,the wound is selected from the group consisting of an incised wound, anopen wound, a surgical or post surgical wound, a diabetic lesion, apressure sore, a bedsore, and a condition or wound related to diabetesor poor circulation.

In some embodiments, the wound is selected from the group consisting ofa non-radiation burn wound, an incised wound, an open wound, a surgicalor post surgical wound, a diabetic lesion, a thermal burn, a chemicalburn, a pressure sore, a bedsore, and a condition related to diabetes orpoor circulation. In some embodiments, the wound is selected from thegroup consisting of an incised wound, an open wound, a surgical or postsurgical wound, a diabetic lesion, a pressure sore, a bedsore, and acondition related to diabetes or poor circulation.

The present disclosure also relates to methods and pharmaceuticalcompositions for reducing scar formation during wound healing in asubject. The pharmaceutical compositions described herein can beadministered directly to the wound or to cells proximate the wound at anamount effective to reduce scar formation in and/or around the wound.Thus, in some embodiments, a method of reducing scar formation duringwound healing in a subject is provided, the method comprisingadministering to the subject a therapeutically effective amount of apharmaceutical composition described herein.

The wound can include any injury to any portion of the body of asubject. According to embodiments, methods are provided to ameliorate,reduce, or decrease the formation of scars in a subject that hassuffered a burn injury. According to preferred embodiments, methods areprovided to treat, reduce the occurrence of, or reduce the probabilityof developing hypertrophic scars in a subject that has suffered an acuteor chronic wound or injury.

Other Disorders

Pharmaceutical compositions described herein may also be used to treatdisorders of abnormal tissue growth and fibrosis including dilativecardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy,pulmonary fibrosis, hepatic fibrosis, glomerulonephritis, and otherrenal disorders.

Combination Radiation Therapy

Pharmaceutical compositions described herein are useful asradiosensitizers. Therefore, pharmaceutical compositions describedherein can be administered in combination with radiation therapy.Radiation therapy is the medical use of high-energy radiation (e.g.,X-rays, gamma rays, charged particles) to shrink tumors and killmalignant cells, and is generally used as part of cancer treatment.Radiation therapy kills malignant cells by damaging their DNA.

Radiation therapy can be delivered to a patient in several ways. Forexample, radiation can be delivered from an external source, such as amachine outside the patient's body, as in external beam radiationtherapy. External beam radiation therapy for the treatment of canceruses a radiation source that is external to the patient, typicallyeither a radioisotope, such as ⁶⁰Co, ¹³⁷Cs, or a high energy X-raysource, such as a linear accelerator. The external source produces acollimated beam directed into the patient to the tumor site.External-source radiation therapy avoids some of the problems ofinternal-source radiation therapy, but it undesirably and necessarilyirradiates a significant volume of non-tumorous or healthy tissue in thepath of the radiation beam along with the tumorous tissue.

The adverse effect of irradiating of healthy tissue can be reduced,while maintaining a given dose of radiation in the tumorous tissue, byprojecting the external radiation beam into the patient at a variety of“gantry” angles with the beams converging on the tumor site. Theparticular volume elements of healthy tissue, along the path of theradiation beam, change, reducing the total dose to each such element ofhealthy tissue during the entire treatment.

The irradiation of healthy tissue also can be reduced by tightlycollimating the radiation beam to the general cross section of the tumortaken perpendicular to the axis of the radiation beam. Numerous systemsexist for producing such a circumferential collimation, some of whichuse multiple sliding shutters which, piecewise, can generate aradio-opaque mask of arbitrary outline.

For administration of external beam radiation, the amount can be atleast about 1 Gray (Gy) fractions at least once every other day to atreatment volume. In a particular embodiment, the radiation isadministered in at least about 2 Gray (Gy) fractions at least once perday to a treatment volume. In another particular embodiment, theradiation is administered in at least about 2 Gray (Gy) fractions atleast once per day to a treatment volume for live consecutive days perweek. In another particular embodiment, radiation is administered in 10Gy fractions every other day, three times per week to a treatmentvolume. In another particular embodiment, a total of at least about 20Gy is administered to a patient in need thereof. In another particularembodiment, at least about 30 Gy is administered to a patient in needthereof. In another particular embodiment, at least about 40 Gy isadministered to a patient in need thereof.

Typically, the patient receives external beam therapy four or five timesa week. An entire course of treatment usually lasts from one to sevenweeks depending on the type of cancer and the goal of treatment. Forexample, a patient can receive a dose of 2 Gy/day over 30 days.

Internal radiation therapy is localized radiation therapy, meaning theradiation source is placed at the site of the tumor or affected area.Internal radiation therapy can be delivered by placing a radiationsource inside or next to the area requiring treatment. Internalradiation therapy is also called brachytherapy. Brachytherapy includesintercavitary treatment and interstitial treatment. In intracavitarytreatment, containers that hold radioactive sources are put in or nearthe tumor. The sources are put into the body cavities. In interstitialtreatment, the radioactive sources alone are put into the tumor. Theseradioactive sources can stay in the patient permanently. Typically, theradioactive sources are removed from the patient after several days. Theradioactive sources are in containers.

There are a number of methods for administration of aradiopharmaceutical agent. For example, the radiopharmaceutical agentcan be administered by targeted delivery or by systemic delivery oftargeted radioactive conjugates, such as a radiolabeled antibody, aradiolabeled peptide and a liposome delivery system. In one particularembodiment of targeted delivery, the radiolabelled pharmaceutical agentcan be a radiolabelled antibody. See, for example, Ballangrud A. M., etal. Cancer Res., 2001; 61:2008-2014 and Goldenber, D. M. J. Nucl. Med.,2002; 43(5):693-713, the contents of which are incorporated by referenceherein.

In another particular embodiment of targeted delivery, theradiopharmaceutical agent can be administered in the form of liposomedelivery systems, such as small unilamellar vesicles, large unilamellarvesicles and multilamellar vesicles. Liposomes can be formed from avariety of phospholipids, such as cholesterol, stearylamine orphosphatidylcholines. See, for example, Emfietzoglou D, Kostarelos K,Sgouros G. An analytical dosimetry study for the use ofradionuclide-liposome conjugates in internal radiotherapy. J Nuci Med2001; 42:499-504, the contents of which are incorporated by referenceherein.

In yet another particular embodiment of targeted delivery, theradiolabeled pharmaceutical agent can be a radiolabeled peptide. See,for example, Weiner R E, Thakur M L. Radiolabeled peptides in thediagnosis and therapy of oncological diseases. Appl Radiat Isot 2002November; 57(5): 49-63, the contents of which are incorporated byreference herein.

In addition to targeted delivery, bracytherapy can be used to deliverthe radiopharmaceutical agent to the target site. Brachy therapy is atechnique that puts the radiation sources as close as possible to thetumor site. Often the source is inserted directly into the tumor. Theradioactive sources can be in the form of wires, seeds or rods.Generally, cesium, iridium or iodine are used.

Systemic radiation therapy is another type of radiation therapy andinvolves the use of radioactive substances in the blood. Systemicradiation therapy is a form of targeted therapy. In systemic radiationtherapy, a patient typically ingests or receives an injection of aradioactive substance, such as radioactive iodine or a radioactivesubstance bound to a monoclonal antibody.

A “radiopharmaceutical agent,” as defined herein, refers to apharmaceutical agent which contains at least one radiation-emittingradioisotope. Radiopharmaceutical agents are routinely used in nuclearmedicine for the diagnosis and/or therapy of various diseases. Theradiolabelled pharmaceutical agent, for example, a radiolabelledantibody, contains a radioisotope (RI) which serves as the radiationsource. As contemplated herein, the term “radioisotope” includesmetallic and non-metallic radioisotopes. The radioisotope is chosenbased on the medical application of the radiolabeled pharmaceuticalagents. When the radioisotope is a metallic radioisotope, a chelator istypically employed to bind the metallic radioisotope to the rest of themolecule. When the radioisotope is a non-metallic radioisotope, thenon-metallic radioisotope is typically linked directly, or via a linker,to the rest of the molecule.

As used herein, a “metallic radioisotope” is any suitable metallicradioisotope useful in a therapeutic or diagnostic procedure in vivo orin vitro. Suitable metallic radioisotopes include, but are not limitedto: Actinium-225, Antimony-124, Antimony-125, Arsenic-74, Barium-103,Barium-140, Beryllium-7, Bismuth-206, Bismuth-207, Bismuth212,Bismuth213, Cadmium-109, Cadmium-115m, Calcium-45, Cerium-139,Cerium-141, Cerium-144, Cesium-137, Chromium-51, Cobalt-55, Cobalt-56,Cobalt-57, Cobalt-58, Cobalt-60, Cobalt-64, Copper-60, Copper-62,Copper-64, Copper-67, Erbium-169, Europium-152, Gallium-64, Gallium-67,Gallium-68, Gadolinium153, Gadolinium-157 Gold-195, Gold-199,Hafnium-175, Hafnium-175−181, Holmium-166, Indium-110, Indium-111,Iridium-192, Iron 55, Iron-59, Krypton85, Lead-203, Lead-210,Lutetium-177, Manganese-54, Mercury-197, Mercury203, Molybdenum-99,Neodymium-147, Neptunium-237, Nickel-63, Niobium95, Osmium-185+191,Palladium-103, Palladium-109, Platinum-195m, Praseodymium-143,Promethium-147, Promethium-149, Protactinium-233, Radium-226,Rhenium-186, Rhenium-188, Rubidium-86, Ruthenium-97, Ruthenium-103,Ruthenium-105, Ruthenium-106, Samarium-153, Scandium-44, Scandium-46,Scandium-47, Selenium-75, Silver-110m, Silver-111, Sodium-22,Strontium-85, Strontium-89, Strontium-90, Sulfur-35, Tantalum-182,Technetium-99m, Tellurium-125, Tellurium-132, Thallium-204, Thorium-228,Thorium-232, Thallium-170, Tin-113, Tin-114, Tin-117m, Titanium-44,Tungsten-185, Vanadium-48, Vanadium-49, Ytterbium-169, Yttrium-86,Yttrium-88, Yttrium-90, Yttrium-91, Zinc-65, Zirconium-89, andZirconium-95.

As used herein, a “non-metallic radioisotope” is any suitablenonmetallic radioisotope (non-metallic radioisotope) useful in atherapeutic or diagnostic procedure in vivo or in vitro. Suitablenon-metallic radioisotopes include, but are not limited to: Iodine-131,Iodine-125, Iodine-123, Phosphorus-32, Astatine-211, Fluorine-18,Carbon-11, Oxygen-15, Bromine-76, and Nitrogen-13.

Identifying the most appropriate isotope for radiotherapy requiresweighing a variety of factors. These include tumor uptake and retention,blood clearance, rate of radiation delivery, half-life and specificactivity of the radioisotope, and the feasibility of large-scaleproduction of the radioisotope in an economical fashion. The key pointfor a therapeutic radiopharmaceutical is to deliver the requisite amountof radiation dose to the tumor cells and to achieve a cytotoxic ortumoricidal effect while not causing unmanageable side-effects.

It is preferred that the physical half-life of the therapeuticradioisotope be similar to the biological half-life of theradiopharmaceutical at the tumor site. For example, if the half-life ofthe radioisotope is too short, much of the decay will have occurredbefore the radiopharmaceutical has reached maximum target/backgroundratio. On the other hand, too long a half-life could cause unnecessaryradiation dose to normal tissues. Ideally, the radioisotope should havea long enough half-life to attain a minimum dose rate and to irradiateall the cells during the most radiation sensitive phases of the cellcycle. In addition, the half-life of a radioisotope has to be longenough to allow adequate time for manufacturing, release, andtransportation.

Other practical considerations in selecting a radioisotope for a givenapplication in tumor therapy are availability and quality. The purityhas to be sufficient and reproducible, as trace amounts of impuritiescan affect the radiolabeling and radiochemical purity of theradiopharmaceutical.

The target receptor sites in tumors are typically limited in number. Assuch, it is preferred that the radioisotope have high specific activity.The specific activity depends primarily on the production method. Tracemetal contaminants must be minimized as they often compete with theradioisotope for the chelator and their metal complexes compete forreceptor binding with the radiolabeled chelated agent.

The type of radiation that is suitable for use in the methods of thepresent invention can vary. For example, radiation can beelectromagnetic or particulate in nature. Electromagnetic radiationuseful in the practice of this invention includes, but is not limitedto, X-rays and gamma rays. Particulate radiation useful in the practiceof this invention includes, but is not limited to, electron beams (betaparticles), protons beams, neutron beams, alpha particles, and negativepi mesons. The radiation can be delivered using conventionalradiological treatment apparatus and methods, and by intraoperative andstereotactic methods. Additional discussion regarding radiationtreatments suitable for use in the practice of this invention can befound throughout Steven A. Leibel et al., Textbook of Radiation Oncology(1998) (publ. W. B. Saunders Company), and particularly in Chapters 13and 14. Radiation can also be delivered by other methods such astargeted delivery, for example by radioactive “seeds,” or by systemicdelivery of targeted radioactive conjugates, J. Padawer et al., CombinedTreatment with Radioestradiol lucanthone in Mouse C3HBA MammaryAdenocarcinoma and with Estradiol lucanthone in an Estrogen Bioassay,Int. J. Radiat. Oncol. Biol. Phys. 7:347-357 (1981). Other radiationdelivery methods can be used in the practice of this invention.

For tumor therapy, both α and β-particle emitters have beeninvestigated. Alpha particles are particularly good cytotoxic agentsbecause they dissipate a large amount of energy within one or two celldiameters. The β-particle emitters have relatively long penetrationrange (2-12 mm in the tissue) depending on the energy level. Thelong-range penetration is particularly important for solid tumors thathave heterogeneous blood flow and/or receptor expression. The β-particleemitters yield a more homogeneous dose distribution even when they areheterogeneously distributed within the target tissue.

In a particular embodiment, therapeutically effective amounts of thepharmaceutical compositions described herein are administered incombination with a therapeutically effective amount of radiation therapyto treat cancer (e.g., lung cancer, such as non-small cell lung cancer).The amount of radiation necessary can be determined by one of skill inthe art based on known doses for a particular type of cancer. See, forexample, Cancer Medicine 5^(th) ed., Edited by R. C. Bast et al., July2000, B C Decker.

Synthetic Methods

Also provided herein are synthetic methods for preparing crystallineforms (e.g., single crystalline forms, such as single crystalline Form Aand single crystalline Form D) of Selinexor.

A seventh embodiment provides a method of preparing a single crystallineform of a compound represented by Structural Formula I, wherein thesingle crystalline form is Form A and is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 4.4°, 19.9°,21.3° and 22.0°. The method comprises:

-   (a) suspending single crystalline Form B, C or D of the compound of    Structural Formula I, or a mixture comprising two or more of single    crystalline Form B, C or D of the compound of Structural Formula I,    in isopropanol or a mixture of isopropanol and water to form a    slurry, wherein single crystalline Form D is characterized by at    least three X-ray powder diffraction peaks at 2θ angles selected    from 3.7°, 7.3°, 10.9°, 18.3° and 21.9°, single crystalline Form B    is characterized by at least three X-ray powder diffraction peaks at    2θ angles selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8° and    single crystalline Form C is characterized by at least three X-ray    diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1° and    18.6°;-   (b) heating the slurry to a temperature less than or equal to about    70° C. to form a second slurry or a solution;-   (c) cooling the second slurry or the solution and adding water to    the second slurry or the solution, thereby forming solid particles    of crystalline Form A of the compound of Structural Formula I; and-   (d) isolating the solid particles of crystalline Form A,    thereby preparing a composition comprising particles of single    crystalline Form A of the compound of Structural Formula I.    Characteristics and alternative characteristics for single    crystalline Form A and single crystalline Forms B, C and D,    including alternative XRPD, DSC and/or TGA characteristics, are as    described above with respect to the first and second embodiments,    respectively. Values and alternative values for d(0.9), d(0.5) and    d(0.1), and ratios thereof, as well as characteristics of the    particle size distribution (e.g., unimodal, normal) of the particles    of single crystalline Form A, are as described in the fifth    embodiment, of any aspect thereof.

In some aspects of the seventh embodiment, the solid particles ofcrystalline Form A have a unimodal particle size distributioncharacterized by a d(0.9) of 70 microns or less. In some aspects of theseventh embodiment, the solid particles of crystalline Form A have aunimodal particle size distribution characterized by a d(0.9) of 100microns or less.

In some aspects of the seventh embodiment, single crystalline Form B, Cor D of the compound of Structural Formula I or a mixture comprising twoor more of the single crystalline forms of the compound of StructuralFormula I, is suspended in a mixture of isopropanol and water in step(a).

In some aspects of the seventh embodiment, the ratio of isopropanol towater by volume in the mixture of isopropanol and water is from about0.1 to about 4, for example, about 1.

In some aspects of the seventh embodiment, single crystalline Form B, Cor D of Structural Formula I or a mixture comprising two or more ofsingle crystalline Forms B, C or D of the compound of Structural FormulaI is suspended in an amount of isopropanol or mixture of isopropanol andwater of from about 5 to about 10 parts by weight with respect to singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more of single crystalline Form B, C or D ofthe compound of Structural Formula I, for example, from about 6 to about7 parts by weight with respect to single crystalline Form B, C or D or amixture comprising two or more of single crystalline Form B, C or D ofthe compound of Structural Formula I.

In some aspects of the seventh embodiment, the slurry is heated to atemperature less than or equal to about 50° C. In some aspects of theseventh embodiment, the slurry is heated to a temperature of from about35° C. to about 70° C. or of from about 35° C. to about 50° C. In someaspects of the seventh embodiment, the slurry is heated to a temperatureof from about 65° C. to about 70° C.

In some aspects of the seventh embodiment, the second slurry or thesolution is cooled to from about 0° C. to about 55° C. For example, thesecond slurry or the solution is cooled to from about 0° C. to about 5°C. to from about 15° C. to about 20° C. or to from about 45° C. to about50° C.

In some aspects of the seventh embodiment, water is added to the secondslurry or the solution in an amount of from about 5 parts by weight toabout 15 parts by weight with respect to single crystalline Form B, C orD of the compound of Structural Formula I or a mixture comprising two ormore of crystalline Forms B, C or D of the compound of StructuralFormula I. For example, water is added to the second slurry or thesolution in an amount of from about 7 parts by weight to about 10 partsby weight or of about 10 parts by weight with respect to singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more of crystalline Forms B, C or D of thecompound of Structural Formula I.

Isolating the solid particles of crystalline Form A is typicallyeffected by filtration and, optionally, rinsing of the filtered solidswith a solvent (e.g., a chilled solvent), although other means ofisolating the solid particles are known in the art. Other means ofisolating the solid particles of crystalline Form A include, but are notlimited to, distilling liquid present in the second slurry or thesolution away from the solid particles or otherwise drying the solidparticles of crystalline Form A, for example, by heating the secondslurry or the solution, by subjecting the second slurry or the solutionto reduced pressure (e.g., in vacuo) or any combination of theforegoing.

A eighth embodiment provides a method of preparing a single crystallineform of a compound represented by Structural Formula I, wherein thesingle crystalline form is Form A and is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 4.4°, 19.9°,21.3° and 22.0°. The method comprises:

-   (a) suspending single crystalline Form B, C or D of the compound of    Structural Formula I or a mixture comprising two or more of single    crystalline Form B, C or D of the compound of Structural Formula I,    in isopropanol or a mixture of isopropanol and water to form a    slurry, wherein single crystalline Form D is characterized by at    least three X-ray powder diffraction peaks at 2θ angles selected    from 3.7°, 7.3°, 10.9°, 18.3° and 21.9°, single crystalline Form B    is characterized by at least three X-ray powder diffraction peaks at    2θ angles selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8° and    single crystalline Form B is characterized by at least three X-ray    diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1° and    18.6°;-   (b) heating the slurry to a temperature less than or equal to about    70° C. to form a second slurry or a solution;-   (c) adding water to the second slurry or the solution and cooling    the second slurry or the solution and thereby forming solid    particles of crystalline Form A of the compound of Structural    Formula I; and-   (d) isolating the solid particles of crystalline Form A,    thereby preparing a composition comprising particles of single    crystalline Form A of the compound of Structural Formula I.    Characteristics and alternative characteristics for single    crystalline Form A and single crystalline Forms B, C and D,    including alternative XRPD, DSC and/or TGA characteristics, are as    described above with respect to the first and second embodiments,    respectively. Values and alternative values for d(0.9), d(0.5) and    d(0.1), and ratios thereof, as well as characteristics of the    particle size distribution (e.g., unimodal, normal) of the particles    of single crystalline Form A, are as described in the fifth    embodiment, of any aspect thereof.

In some aspects of the eighth embodiment, the solid particles ofcrystalline Form A have a unimodal particle size distributioncharacterized by a d(0.9) of 70 microns or less. In some aspects of theeighth embodiment, the solid particles of crystalline Form A have aunimodal particle size distribution characterized by a d(0.9) of 100microns or less.

In some aspects of the eight embodiment, single crystalline Form B, C orD of the compound of Structural Formula I or a mixture comprising two ormore of the single crystalline forms of the compound of StructuralFormula I, is suspended in a mixture of isopropanol and water in step(a).

In some aspects of the eighth embodiment, the ratio of isopropanol towater by volume in the mixture of isopropanol and water is from about0.1 to about 4, for example, about 1.

In some aspects of the eighth embodiment, single crystalline Form B, Cor D of Structural Formula I or a mixture comprising two or more ofsingle crystalline Form B, C or D of the compound of Structural FormulaI is suspended in an amount of isopropanol or mixture of isopropanol andwater of from about 5 to about 10 parts by weight with respect to singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more of single crystalline Form B, C or D ofthe compound of Structural Formula I, for example, from about 6 to about7 parts by weight with respect to single crystalline Form B, C or D or amixture comprising two or more of single crystalline Form B, C or D ofthe compound of Structural Formula I.

In some aspects of the eighth embodiment, the slurry is heated to atemperature less than or equal to about 50° C. In some aspects of theeighth embodiment, the slurry is heated to a temperature of from about35° C. to about 70° C. or of from about 35° C. to about 50° C. In someaspects of the seventh embodiment, the slurry is heated to a temperatureof from about 65° C. to about 70° C.

In some aspects of the eighth embodiment, the second slurry or thesolution is cooled to from about 0° C. to about 55° C. For example, thesecond slurry or the solution is cooled to from about 0° C. to about 5°C., to from about 15° C. to about 20° C. or to from about 45° C. toabout 50° C.

In some aspects of the eighth embodiment, water is added to the secondslurry or the solution in an amount of from about 5 parts by weight toabout 15 parts by weight with respect to single crystalline Form B, C orD of the compound of Structural Formula I or a mixture comprising two ormore of crystalline Form B, C or D of the compound of Structural FormulaI. For example, water is added to the second slurry or the solution inan amount of from about 7 parts by weight to about 10 parts by weight orof about 10 parts by weight with respect to single crystalline Form D,or single crystalline Form B, C or D of the compound of StructuralFormula I or a mixture comprising two or more of crystalline Form B, Cor D of the compound of Structural Formula I.

Isolating the solid particles of crystalline Form A is typicallyeffected by filtration and, optionally, rinsing of the filtered solidswith a solvent (e.g., a chilled solvent), although other means ofisolating the solid particles are known in the art. Other means ofisolating the solid particles of crystalline Form A include, but are notlimited to, distilling liquid present in the second slurry or thesolution away from the solid particles or otherwise drying the solidparticles of crystalline Form A, for example, by heating the secondslurry or the solution, by subjecting the second slurry or the solutionto reduced pressure (e.g., in vacuo) or any combination of theforegoing.

A ninth embodiment provides a method of preparing a single crystallineform of a compound represented by Structural Formula I, wherein thesingle crystalline form is Form A and is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 4.4°, 19.9°,21.3° and 22.0°. The method comprises: heating single crystalline FormB, C or D of the compound of Structural Formula I or a mixturecomprising two or more of crystalline Form B, C or D of the compound ofStructural Formula I and inducing formation of solid particles ofcrystalline Form A; or maturing single crystalline Form B, C or D of thecompound of Structural Formula I, or a mixture comprising two or more ofcrystalline Form B, C or D of the compound of Structural Formula I in asolvent system and inducing formation of solid particles of crystallineForm A; or drying single crystalline Form B, C or D of the compound ofStructural Formula I, or a mixture comprising two or more of crystallineForm B, C or D of the compound of Structural Formula I, thereby formingsolid particles of crystalline Form A; orally combination of theforegoing; and isolating the solid particles of crystalline Form A,thereby preparing single crystalline Form A of the compound ofStructural Formula I. Characteristics and alternative characteristicsfor single crystalline Forms A, B, C and D including alternative XRPD,DSC and/or TGA characteristics, are as described above with respect tothe first, second, third and fourth embodiments, respectively. Valuesand alternative values for d(0.9), d(0.5) and d(0.1), and ratiosthereof, as well as characteristics of the particle size distribution(e.g., unimodal, normal) of the particles of single crystalline Form A,are as described in the fifth embodiment, of any aspect thereof.

In some aspects of the ninth embodiment, the method comprises:

-   heating a mixture comprising two or more crystalline forms of the    compound of Structural Formula I and inducing formation of solid    particles of crystalline Form A; or-   maturing a mixture comprising two or more crystalline forms of the    compound of Structural Formula I in a solvent system and inducing    formation of solid particles of crystalline Form A; or-   drying a mixture comprising two or more crystalline forms of the    compound of Structural Formula I, thereby forming solid particles of    crystalline Form A; or

any combination of the foregoing.

In some aspects of this aspect, the mixture comprises two or morecrystalline forms selected from Form A, Form B, Form C or Form D, morespecifically, two or more crystalline forms selected from Form B, Form Cor Form D. In some aspects of this aspect, one of the two or morecrystalline forms is Form B, Form C or Form D. In some aspects of thisaspect, the mixture does not comprise Form A.

In alternative aspects of the ninth embodiment, the method comprises:heating single crystalline Form B, C or D of the compound of StructuralFormula I and inducing formation of solid particles of crystalline FormA; or maturing single crystalline Form B, C or D of the compound ofStructural Formula I in a solvent system and inducing formation of solidparticles of crystalline Form A; or drying single crystalline Form B, Cor D of the compound of Structural Formula I, thereby forming solidparticles of crystalline Form A; or any combination of the foregoing. Insome aspects of this aspect, the single crystalline form is Form B. Inother aspects of this aspect, the single crystalline form is Form C. Inyet other aspects of this aspect, the single crystalline form is Form D.

“Inducing formation,” used herein, includes any conditions that inducethe compound of Structural Formula I to crystallize as the specifiedcrystalline form, for example, crystalline Form A or crystalline Form D.Inducing formation includes merely allowing solid particles of thespecified crystalline form to precipitate from a solution or slurry, forexample, without actively performing any step. Inducing formation alsoincludes maturing (e.g., aging, with or without cooling, and/or cycling)a solution comprising a compound of Structural Formula I in anappropriate solvent system and/or allowing a solution comprising acompound of Structural Formula I in an appropriate solvent system toslowly evaporate, with or without cooling. Inducing formation alsoincludes cooling the compound of Structural Formula I or a solutionincluding the compound of Structural Formula I. Other methods ofinducing formation of a crystalline solid are known in the art andinclude, for example, seeding, and/or using anti-solvents and vapordiffusion. In preferred embodiments, inducing formation comprisescooling the compound of Structural Formula I or a solution or slurryincluding the compound of Structural Formula I in an appropriate solventsystem.

“Solvent system,” as used herein, refers to a single solvent or amixture of two or more (typically, two) different solvents. Exemplarysolvents for a solvent system include water and organic solvents suchas, but not limited to, methanol, s-butanol, m-butanol, i-butanol,cyclopentylmethylether, cyclopentylethylether, heptane, 1,4-dioxane,1,2-dimethoxyethane, 1,2-dichloroethane, toluene, cumene, diisopropylether, anisole, dichloromethane, tetrahydrofuran,2-methyltetrahydrofuran, tert-butanol, 2-propanol ethanol, ethylacetate, isopropyl acetate, nitromethane, acetonitrile,dimethylsulfoxide, tert-butylmethyl ether (TBME), methylisobutylketone(MIBK), propyl acetate, butyl acetate, dimethoxyethane, isooctane andpropionitrile.

Preferred solvent systems for inducing formation of crystalline Form Ainclude propionitrile, isopropanol, n-propanol, a mixture of isopropanoland water and a mixture of 2-methyltetrahydrofuran and isooctane,heptane, toluene or acetonitrile (comprising less than or about 20%acetonitrile by volume). A particularly preferred solvent system forinducing formation of solid particles of crystalline Form A is a mixtureof isopropanol and water (e.g., comprising from about 20% to about 50%isopropanol by volume). The solvent system for inducing formation ofsolid particles of crystalline Form A at temperatures below 50° C.should not be nitromethane, acetonitrile, or a mixture of acetonitrileand a second solvent comprising greater than about 20% acetonitrile byvolume.

Typically, when single crystalline Form B, C or D of the compound ofStructural Formula I or a mixture comprising two or more of crystallineForm B, C or D of the compound of Structural Formula I is heated, singlecrystalline Form B, C or D of the compound of Structural Formula I orthe mixture comprising two or more of crystalline Form B, C or D of thecompound of Structural Formula I is heated in a solvent system, forexample, an aqueous mixture of isopropanol and water. However, singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more of crystalline Form B, C or D of thecompound of Structural Formula I can also be heated neat (in the absenceof solvent). A preferred solvent system for heating single crystallineForm B, C or D of the compound of Structural Formula I or the mixturecomprising two or more crystalline forms of the compound of StructuralFormula I is a mixture of isopropanol in water (e.g., a mixturecomprising from about 20% to about 50% isopropanol by volume).

“Maturing,” as used herein, includes both aging single crystalline FormB, C or D of the compound of Structural Formula I or a mixturecomprising two or more crystalline forms of the compound of StructuralFormula I in a solvent system (with or without slow evaporation), forexample, under substantially constant conditions (e.g., ambienttemperature and pressure) for a period of time (e.g., less than 30minutes, less than 1 hour, at least 30 minutes, at least 1 hour, atleast 4 hours, at least 12 hours, at least 1 day, at least 7 days), andcycling single crystalline Form B, C or D of the compound of StructuralFormula I or a mixture comprising two or more crystalline forms of thecompound of Structural Formula I in a solvent system, for example,between two or more temperatures over a period of time (e.g., betweenroom temperature and 50° C. every four hours).

Preferred solvent systems for maturing single crystalline Form B, C or Dof the compound of Structural Formula I or a mixture comprising two ormore crystalline forms of the compound of Structural Formula I includeethyl acetate, a mixture of isopropanol and water (e.g., a mixturecomprising from about 20% to about 50% isopropanol by volume) and amixture of ethanol and water.

“Room temperature” and “ambient temperature,” as used herein, means atemperature of from about 16° C. to about 25° C.

“Ambient conditions,” as used herein, refers to room temperature andatmospheric pressure conditions.

Drying single crystalline Form B, C or D of the compound of StructuralFormula I or a mixture comprising two or more of crystalline Form B, Cor D of the compound of Structural Formula I can be accomplished, forexample, by distilling any liquid present away from the solidcrystalline form(s), by exposing the solid crystalline form(s) toambient conditions or passing a stream of gas, such as nitrogen gas,over the solid crystalline form(s) (and thereby inducing the evaporationor desolvation of any liquid or entrapped volatile substance, such asacetonitrile), by subjecting the solid crystalline form(s) to reducedpressure (e.g., in vacuo) or any combination of the foregoing. Singlecrystalline Form D, in particular, can be converted to singlecrystalline Form A by drying under conditions in which acetonitrile candesolvate from single crystalline Form D, for example, by subjectingsingle crystalline Form D to reduced pressure (e.g., in vacuo) or byexposing single crystalline Form D to ambient conditions or passing astream of gas over single crystalline Form D.

It is understood that, quite often, in practice, the steps for preparingsingle crystalline form A according to the methods described hereinentail a combination of heating, maturing and/or drying. For example,when a mixture comprising two or more crystalline forms of the compoundof Structural Formula I is aged, for example, at 50° C. for 72 hours upto 1 week, the method of preparing single crystalline Form A comprisesheating and maturing. When single crystalline Form D of the compound ofStructural Formula I is placed in vacuo at 35° C., the method ofpreparing single crystalline Form A comprises drying and heating.

Isolating the solid particles of crystalline Form A can be effected byfiltration and, optionally, rinsing of the filtered solids with asolvent (e.g., a chilled solvent), although other means of isolatingsolid particles are known in the art. Other means of isolating the solidparticles of crystalline Form A include, but are not limited to,distilling any liquid present away from the solid particles or otherwisedrying the solid particles of crystalline Form A, for example, byheating a slurry or solution containing the particles (to induceevaporation of any liquid or volatile substance), by subjecting a slurryor solution to reduced pressure (e.g., in vacuo), by passing a stream ofgas (e.g., nitrogen) over the sample, or any combination of theforegoing.

A tenth embodiment provides a method of preparing a single crystallineform of a compound represented by Structural Formula I wherein thesingle crystalline form is Form D and is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 3.7°, 7.3°,10.9°, 18.3° and 21.9°. The method comprises:

dissolving the compound of Structural Formula I in a solvent systemcomprising acetonitrile;

inducing formation of solid particles of crystalline Form D of thecompound of Structural Formula I or single crystalline Form B or C ofthe compound of Structural Formula I or a mixture comprising two or moreof crystalline Form B, C or D of the compound of Structural Formula I;and

isolating the solid particles of crystalline Form B, C or D ofStructural Formula I or a mixture comprising two or more of crystallineForm B, C or D of the compound of Structural Formula I, therebypreparing single crystalline Form B, C or D of the compound ofStructural Formula I or a mixture comprising two or more of crystallineForm B, C or D of the compound of Structural Formula I of the compoundof Structural Formula I. Characteristics and alternative characteristicsfor single crystalline Form D, including alternative XRPD, DSC and/orTGA characteristics, are as described above with respect to the secondembodiment.

Preferred solvent systems for the method provided by the tenthembodiment include acetonitrile and mixtures of acetonitrile and asecond solvent selected from water, 2-methyltetrahydrofuran, ethylacetate or a combination of the foregoing, containing greater than 20%by volume acetonitrile, such as greater than or about 40% acetonitrileor greater than or about 95% acetonitrile.

In some aspects of the tenth embodiment, inducing formation of solidparticles of crystalline Form D comprises cooling the solution of thecompound of Structural Formula I in the solvent system. Preferredsolvent systems for inducing formation of solid particles of crystallineForm D include acetonitrile and mixtures of acetonitrile and a secondsolvent selected from water, 2-methyltetrahydrofuran, ethyl acetate or acombination of the foregoing, containing greater than 20% by volumeacetonitrile, such as at least or about 40% acetonitrile or at least orabout 95% acetonitrile.

Isolating the solid particles of single crystalline Form B, C or D ofthe compound of Structural Formula I or a mixture comprising two or moreof crystalline Form B, C or D of the compound of Structural Formula I,is preferably achieved by filtration and, optionally, rinsing of thefiltered solids with a solvent (e.g., a chilled solvent). For example,single crystalline Form D can be isolated by filtration and rinsing ofthe filtered solids with acetonitrile, for example, cold acetonitrile.

Other means of isolating the solid particles include, but are notlimited to, distilling any liquid present away from the solid particlesor otherwise drying the solid particles, for example, by heating aslurry or solution containing the particles (to induce evaporation ofany liquid or volatile substance), by subjecting a slurry or solution toreduced pressure (e.g., in vacuo), by passing a stream of gas (e.g.,nitrogen) over the crystalline form, or any combination of theforegoing. However, as described in the Exemplification, crystallineForm D can desolvate under a variety of conditions. Thus, extendedheating or being subjected to reduced pressures for an extended periodof time can cause crystalline Form D to convert to crystalline Form B, Cand/or A or mixtures thereof. One skilled in the art will be able todetermine how to isolate crystalline Form D without undueexperimentation using the guidance provided herein.

In some aspects of the tenth embodiment, single crystalline Form D is inthe form of a solvate, for example, an acetonitrile solvate. Moreparticularly, the solvate (e.g., acetonitrile solvate) comprises fromabout 0.5 to about 1.5 molar equivalents of solute (e.g., acetonitrile)per molar equivalent of the compound of Structural Formula I, yet moreparticularly, one molar equivalent of solute per molar equivalent of thecompound of Structural Formula I.

In some aspects of the tenth embodiment, the solvent system comprisesgreater than 20% by volume acetonitrile, for exaniple, at least or about40% by volume acetonitrile, or at least or about 95% by volumeacetonitrile.

An eleventh embodiment provides a method of preparing a compound ofStructural Formula I. The method comprises:

-   combining a trialkylamine, 2-methyltetrahydrefuran, a compound of    Structural Formula II:

-   -   and a compound of Structural Formula III:

-   -   to form a reaction mixture;

-   cooling the reaction mixture to from about −80° C. to about 0° C.;

-   treating the reaction mixture with propylphosphonic anhydride to    provide a mixture comprising the compound of Structural Formula I;    and

-   isolating the compound of Structural Formula I from the mixture.

“Trialkylamine,” as used herein, means N(R)₃, wherein each R isindependently selected from C₁-C₄ alkyl. Exemplary triethylaminesincludes triethylamine and diisopropylethylamine. A preferredtrialkylamine is diisopropylethylamine.

In some aspects of the eleventh embodiment, the method comprises coolingthe reaction mixture to from about −50° C. to about −15° C., moreparticularly, from about −25° C. to about −20° C.

In some aspects of the eleventh embodiment, isolating the compound ofStructural Formula I from the mixture comprises adding an aqueous quenchsolution to the mixture comprising the compound of Structural Formula Iand performing an extractive work-up of the resulting quenched reactionmixture. For example, an aqueous quench solution of water or a neutral(e.g., pH 7) buffer, such as phosphate buffer, preferably water, can beadded to the reaction mixture, and an extractive work-up of theresulting quenched reaction mixture performed.

Methods of performing extractive work-ups are within the skill of one ofordinary skill in the art. For example, an extractive work-up of thereaction mixture comprising the compound of Structural Formula I caninclude separating the aqueous and organic layers resulting from theaddition of the aqueous quench solution to the reaction mixture and,optionally, washing the organic layer, for example, with a dilute(approximately 6% w/w sodium chloride) brine solution and with water. Insome aspects of the eleventh embodiment comprising an extractivework-up, the extractive work-up comprises washing the quenched reactionmixture with an aqueous solution of sodium chloride.

Isolating the compound of Structural Formula I from the reaction mixturecan alternatively or further include any of the techniques and methodsfor isolating crystalline forms of the compound of Structural Formula Idescribed above with respect to the seventh,eighth, ninth and tenthembodiments.

It will be understood that, although described independently of oneanother, the methods described in embodiments ten and eleven can beperformed in sequence (i.e., eleven then ten) to prepare singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more of crystalline Form B, C or D of thecompound of Structural Formula I. It will also be understood that,although described independently of one another, the methods describedin embodiments seven or nine, ten and eleven can be performed insequence (i.e., eleven, then ten, then seven or nine) to prepare singlecrystalline Form A of the compound of Structural Formula I.

Thus, in some aspects of the tenth embodiment, including the tenthembodiment and any aspect thereof described hereinabove, the methodfurther comprises combining a trialkylamine, 2-methyltetrahydrofuran, acompound of Structural Formula II and a compound of Structural FormulaIII to form a reaction mixture; cooling the reaction mixture to fromabout −80° C. to about 0° C.; treating the reaction mixture withpropylphosphonic anhydride to provide a mixture comprising the compoundof Structural Formula I; and isolating the compound of StructuralFormula I from the mixture. Alternative conditions for these furthersteps, including further details related to the trialkylamine, thetemperature of the reaction mixture and the isolation of the compound ofStructural Formula I, can be found in the eleventh embodiment, or anyaspect thereof.

In some aspects of the seventh, eighth and ninth embodiments, includingthe seventh embodiment, the eighth embodiment, the ninth embodiment andany aspect of the foregoing, the method further comprises combining atrialkylamine, 2-methyltetrahydrofuran, a compound of Structural FormulaII and a compound of Structural Formula III to form a reaction mixture;cooling the reaction mixture to from about −80° C. to about 0° C.;treating the reaction mixture with propylphosphonic anhydride to providea mixture comprising the compound of Structural Formula I; isolating thecompound of Structural Formula I from the reaction mixture; dissolvingthe isolated compound of Structural Formula I in a solvent systemcomprising acetonitrile; and inducing formation of solid particles ofcrystalline Form D of the compound of Structural Formula I and isolatingthe solid particles of crystalline Form D to obtain single crystallineForm B, C or D of the compound of Structural Formula I or a mixturecomprising two or more of crystalline Form B, C or D of the compound ofStructural Formula I. Alternative conditions for these further steps,including further details related to the trialkylamine, the temperatureof the reaction mixture, the isolation of the compound of StructuralFormula I, the solvent system and inducing formation and isolating solidparticles of crystalline Form D, can be found in the tenth and eleventhembodiments, or any aspect of the foregoing. Characteristics andalternative characteristics for single crystalline Form D, includingalternative XRPD, DSC and/or TGA characteristics, are as described abovewith respect to the second embodiment.

An twelfth embodiment provides a method of preparing a singlecrystalline form of a compound represented by Structural Formula Iwherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9°, 21.3° and 22.0°. The method comprises:

-   (a) combining a trialkylamine, 2-methyltetrahydrofuran, a compound    of Structural Formula II:

-   -   and a compound of Structural Formula III:

-   -   to form a reaction mixture;    -   (b) cooling the reaction mixture to from about −80° C. to about        0° C.;    -   (c) treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I;    -   (d) isolating the compound of Structural Formula I from the        mixture;    -   (e) dissolving the isolated compound of Structural Formula I in        a solvent system comprising acetonitrile;    -   (f) inducing formation of solid particles of crystalline Form D        of the compound of Structural Formula I and isolating the solid        particles of crystalline Form D to obtain single crystalline        Form D of the compound of Structural Formula I, or solid        particles of single crystalline Form B or C of Structural        Formula I or a mixture comprising two or more of single        crystalline Form B, C or D of the compound of Structural Formula        I wherein single crystalline Form D is characterized by at least        three X-ray powder diffraction peaks at 2θ angles selected from        3.7°, 7.3°, 10.9°, 18.3° and 21.9°, Form B is characterized by        at least three X-ray powder diffraction peaks at 2θ angles        selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single        crystalline Form B is characterized by at least three X-ray        diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1°        and 18.6°;    -   (g) heating single crystalline Form D, or single crystalline        Form B or C of Structural Formula I or a mixture comprising two        or more single crystalline forms of the compound of Structural        Formula I, and inducing formation of solid particles of        crystalline Form A; or maturing single crystalline Form D, or        single crystalline Form B or C of Structural Formula I or a        mixture comprising two or more of single crystalline Form B, C        or D of the compound of Structural Formula I, in a solvent        system and inducing formation of solid particles of crystalline        Form A; or drying single crystalline Form D or single        crystalline Form B or C of Structural Formula I or a mixture        comprising two or more of single crystalline Form B, C or D of        the compound of Structural Formula I, thereby forming solid        particles of crystalline Form A; or any combination of the        foregoing; and    -   (h) isolating the particles of crystalline Form A,        thereby preparing single crystalline Form A of the compound of        Structural Formula I. Conditions and alternative conditions for        the steps can be found in the ninth, tenth and eleventh        embodiments, or any aspect of the foregoing. Characteristics and        alternative characteristics for single crystalline Form A and        single crystalline Forms B, C and D, including alternative XRPD,        DSC and/or TGA characteristics, are as described above with        respect to the other embodiments.

A thirteenth embodiment provides a method of preparing a singlecrystalline form of a compound represented by Structural Formula Iwherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4°, 19.9°, 21.3° and 22.0°. The method comprises:

-   -   (a) combining a trialkylamine, 2-methyltetrahydrofuran, a        compound of Structural Formula II:

-   -   -   and a compound of Structural Formula III:

-   -   -   to form a reaction mixture;

    -   (b) cooling the reaction mixture to from about −80° C. to about        0° C.;

    -   (c) treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I;

    -   (d) exchanging solvent of the reaction mixture comprising the        compound of Structural Formula I for a solvent system comprising        acetonitrile;

    -   (e) inducing formation of solid particles of crystalline Form D        of the compound of Structural Formula I and isolating the solid        particles of crystalline Form D to obtain single crystalline        Form D of the compound of Structural Formula I, or solid        particles of single crystalline Form B or C of Structural        Formula I or a mixture comprising two or more of single        crystalline Form B, C or D of the compound of Structural Formula        I wherein single crystalline Form D is characterized by at least        three X-ray powder diffraction peaks at 2θ angles selected from        3.7°, 7.3°, 10.9°, 18.3° and 21.9°, Form B is characterized by        at least three X-ray powder diffraction peaks at 2θ angles        selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single        crystalline Form C is characterized by at least three X-ray        diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1°        and 18.6°;

    -   (f) heating single crystalline Form D, or single crystalline        Form B or C of Structural Formula I or a mixture comprising two        or more of single crystalline Form B, C or D of the compound of        Structural Formula I, and inducing formation of solid particles        of crystalline Form A; or maturing single crystalline Form D, or        single crystalline Form B or C of Structural Formula or a        mixture comprising two or more of single crystalline Form B, C        or D of the compound of Structural Formula I, in a solvent        system and inducing formation of solid particles of crystalline        Form A; or drying single crystalline Form D or single        crystalline Form B or C of Structural Formula I or a mixture        comprising two or more single of crystalline Form B, C or D of        the compound of Structural Formula I, thereby forming solid        particles of crystalline Form A; or any combination of the        foregoing; and

    -   (g) isolating the particles of crystalline Form A,        thereby preparing single crystalline Form A of the compound of        Structural Formula I. Conditions and alternative conditions for        the steps can be found in the ninth, tenth and eleventh        embodiments, or any aspect of the foregoing. Characteristics and        alternative characteristics for single crystalline Form A and        single crystalline Forms B, C and D, including alternative XRPD,        DSC and/or TGA characteristics, are as described above with        respect to other embodiments.

Distilligraphic exchange is a suitable solvent exchange method for anyembodiments having a solvent exchange step.

A fourteenth embodiment provides a method for preparing a compositioncomprising particles of a single crystalline form of a compoundrepresented by Structural Formula I wherein the single crystalline formis Form A and is characterized by at least three X-ray powderdiffraction peaks at 2θ angles selected from 4.4°, 19.9°, 21.3° and22.0°; and the particles have a particle size distribution characterizedby a d(0.9) of less than about 70 microns. In some aspects of thefourteenth embodiment, the particles have a particle size distributioncharacterized by a d(0.9) of less than about 100 microns.

In some embodiments, the method comprises:

-   -   (a) combining a trialkylamine, 2-methyltetrahydrefuran, a        compound of Structural Formula II:

-   -   -   and a compound of Structural Formula III:

-   -   -   to form a reaction mixture;

    -   (b) cooling the reaction mixture to from about −80° C. to about        0° C.;

    -   (c) treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I;

    -   (d) isolating the compound of Structural Formula I from the        mixture;

    -   (e) dissolving the isolated compound of Structural Formula I in        a solvent system comprising acetonitrile;

    -   (f) inducing formation of solid particles of crystalline Form D        of the compound of Structural Formula I and isolating the solid        particles of crystalline Form D to obtain single crystalline        Form D of the compound of Structural Formula I, or solid        particles of single crystalline Form B or C of Structural        Formula I or a mixture comprising two or more of single        crystalline Form B, C or D of the compound of Structural Formula        I wherein single crystalline Form D is characterized by at least        three X-ray powder diffraction peaks at 2θ angles selected from        3.7°, 7.3°, 10.9°, 18.3° and 21.9°, Form B is characterized by        at least three X-ray powder diffraction peaks at 2θ angles        selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single        crystalline Form C is characterized by at least three X-ray        diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1°        and 18.6°;

    -   (g) suspending single crystalline Form B, C or D of the compound        of Structural Formula I or a mixture comprising two or more of        single crystalline Form B, C or D of the compound of Structural        Formula I, in isopropanol or a mixture of isopropanol and water        to form a slurry; (h) heating the slurry to a temperature less        than or equal to about 70° C. to form a second shiny or a        solution;

    -   (i) cooling the second slurry or the solution and adding water        to the second slurry or the solution, thereby forming solid        particles of crystalline Form A of the compound of Structural        Formula I; and

    -   (j) isolating the solid particles of crystalline Form A, thereby        preparing a composition comprising particles of single        crystalline From A of the compound of Structural Formula I.

In some embodiments, the method comprises:

-   -   (a) combining a trialkylamine, 2-methyltetrahydrofuran, a        compound of Structural Formula II:

-   -   -   and a compound of Structural Formula III:

-   -   -   to form a reaction mixture;

    -   (b) cooling the reaction mixture to from about −80° C. to about        0° C.;

    -   (c) treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I;

    -   (d) exchanging solvent of the reaction mixture comprising the        compound of Structural Formula I for a solvent system comprising        acetonitrile;

    -   (e) inducing formation of solid particles of crystalline Form D        of the compound of Structural Formula I and isolating the solid        particles of crystalline Form D to obtain single crystalline        Form D of the compound of Structural Formula I, or solid        particles of single crystalline Form B or C of Structural        Formula I or a mixture comprising two or more of single        crystalline Form B, C or D of the compound of Structural Formula        I wherein single crystalline Form D is characterized by at least        three X-ray powder diffraction peaks at 2θ angles selected from        3.7°, 7.3°, 10.9°, 18.3° and 21.9°, Form B is characterized by        at least three X-ray powder diffraction peaks at 2θ angles        selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single        crystalline Form C is characterized by at least three X-ray        diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1°        and 18.6°;

    -   (f) suspending single crystalline Form D, or single crystalline        Form B or C of the compound of Structural Formula I or a mixture        comprising two or more of single crystalline Form B, C or D of        the compound of Structural Formula I, in isopropanol or a        mixture of isopropanol and water to form a slurry;

    -   (g) heating the slurry to a temperature less than or equal to        about 70° C. to form a second slurry or a solution;

    -   (h) cooling the second slurry or the solution and adding water        to the second shiny or the solution, thereby forming solid        particles of crystalline Form A of the compound of Structural        Formula I; and

    -   (i) isolating the solid particles of crystalline Form A, thereby        preparing a composition comprising particles of single        crystalline From A of the compound of Structural Formula I.

In some embodiments, the method comprises:

-   -   (a) combining a trialkylamine, 2-methyltetrahydrefuran, a        compound of Structural Formula II:

-   -   -   and a compound of Structural Formula III:

-   -   -   to form a reaction mixture;

    -   (b) cooling the reaction mixture to from about −80° C. to about        0° C.;

    -   (c) treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I;

    -   (d) isolating the compound of Structural Formula I from the        mixture;

    -   (e) dissolving the isolated compound of Structural Formula I in        a solvent system comprising acetonitrile;

    -   (f) inducing formation of solid particles of crystalline Form D        of the compound of Structural Formula I and isolating the solid        particles of crystalline Form D to obtain single crystalline        Form D of the compound of Structural Formula I, or solid        particles of single crystalline Form B or C of Structural        Formula I or a mixture comprising two or more of single        crystalline Form B, C or D of the compound of Structural Formula        I wherein single crystalline Form D is characterized by at least        three X-ray powder diffraction peaks at 2θ angles selected from        3.7°, 7.3°, 10.9°, 18.3° and 21.9°, Form B is characterized by        at least three X-ray powder diffraction peaks at 2θ angles        selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single        crystalline Form C is characterized by at least three X-ray        diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1°        and 18.6°;

    -   (g) suspending single crystalline Form D, or single crystalline        Form B or C of the compound of Structural Formula I or a mixture        comprising two or more of crystalline Form B, C or D of the        compound of Structural Formula I, in isopropanol or a mixture of        isopropanol and water to form a slurry;

    -   (h) heating the slurry to a temperature less than or equal to        about 70° C. to form a second slurry or a solution;

    -   (i) adding water to the second slurry or the solution and        cooling the second slurry or the solution, thereby forming solid        particles of crystalline Form A of the compound of Structural        Formula I; and

    -   (j) isolating the solid particles of crystalline Form A,        thereby preparing a composition comprising particles of single        crystalline Form A of the compound of Structural Formula I.        Conditions and alternative conditions for the steps can be found        in the seventh, eighth, tenth and eleventh embodiments, or any        aspect of the foregoing. Characteristics and alternative        characteristics for single crystalline Form A and single        crystalline Form D, including alternative XRPD, DSC and/or TGA        characteristics, are as described above with respect to the        first and second embodiments, respectively.

In some embodiments In some embodiments, the method comprises:

-   -   (a) combining a trialkylamine, 2-methyltetrahydrofuran, a        compound of Structural Formula II:

-   -   -   and a compound of Structural Formula III:

-   -   -   to form a reaction mixture;

    -   (b) cooling the reaction mixture to from about −80° C. to about        0° C.;

    -   (c) treating the reaction mixture with propylphosphonic        anhydride to provide a mixture comprising the compound of        Structural Formula I;

    -   (d) exchanging solvent of the reaction mixture comprising the        compound of Structural Formula I for a solvent system comprising        acetonitrile;

    -   (e) inducing formation of solid particles of crystalline Form D        of the compound of Structural Formula I and isolating the solid        particles of crystalline Form D to obtain single crystalline        Form D of the compound of Structural Formula I, or solid        particles of single crystalline Form B or C of Structural        Formula I or a mixture comprising two or more of single        crystalline Form B, C or D of the compound of Structural Formula        I wherein single crystalline Form D is characterized by at least        three X-ray powder diffraction peaks at 2θ angles selected from        3.7°, 7.3°, 10.9°, 18.3° and 21.9°, Form B is characterized by        at least three X-ray powder diffraction peaks at 2θ angles        selected from 9.4°, 11.1°, 16.5°, 18.3° and 18.8°. Single        crystalline Form C is characterized by at least three X-ray        diffraction peaks at 2θ angles selected from 3.7°, 11.2°, 12.1°        and 18.6°;

    -   (f) suspending single crystalline Form D, or single crystalline        Form B or C of the compound of Structural Formula I or a mixture        comprising two or more of crystalline Form B, C or D of the        compound of Structural Formula I, in isopropanol or a mixture of        isopropanol and water to form a slurry;

    -   (g) heating the slurry to a temperature less than or equal to        about 70° C. to form a second slurry or a solution;

    -   (h) adding water to the second slurry or the solution and        cooling the second slurry or the solution, thereby forming solid        particles of crystalline Form A of the compound of Structural        Formula I; and

    -   (i) isolating the solid particles of crystalline Form A,        thereby preparing a composition comprising particles of single        crystalline Form A of the compound of Structural Formula I.

EXEMPLIFICATION Example 1 Preparation of Selinexor Lot No. 1305365 (FormA)

Selinexor for Lot No. 1305365 was made in accordance with the followingreaction scheme:

A solution of propane phosphonic acid anhydride (T3P®, 50% in ethylacetate, 35 Kg) in THF (24.6 Kg) was cooled to about −40° C. To thissolution was added a solution of KG1 (13.8 Kg) and diisopropylethyiamine(12.4 Kg) in tetrahydrofuran (THF, 24.6 Kg). The resulting mixture wasstirred at about −40° C. for approximately 2.5 hours.

In a separate vessel, KJ8 (4.80 Kg) was mixed with THF (122.7 Kg), andthe resulting mixture cooled to about −20° C. The cold activated estersolution was then added to the KJ8 mixture with stirring, and thereaction was maintained at about −20° C. The mixture was warmed to about5° C., water (138.1 Kg) was added and the temperature adjusted to about20° C. After agitating for about an hour, the lower phase was allowed toseparate from the mixture and discarded. The upper layer was dilutedwith ethyl acetate (EtOAc). The organic phase was then washed threetimes with potassium phosphate dibasic solution (˜150 Kg), then withwater (138.6 Kg).

The resulting organic solution was concentrated under reduced pressureto 95 L, EtOAc (186.6 Kg) was added and the distillation repeated to avolume of 90 L. Additional EtOAc (186.8 Kg) was added and thedistillation repeated a third time to a volume of 90 L. The batch wasfiltered to clarify, further distilled to 70 L, then heated to about 75°C., and slowly cooled to 0 to 5° C. The resulting slurry was filteredand the filter cake washed with a mixture of EtOAc (6.3 Kg) and toluene(17.9 Kg) before being dried in a vacuum oven to provide selinexordesignated Lot No. 1305365 (Form A).

Example 2 Preparation of Selinexor Lot No. 1341-AK-109-2 (Form A)

The acetonitrile solvate of selinexor was prepared in accordance withExample 6.

The acetonitrile solvate of selinexor (2.7 g) was suspended in a mixtureof isopropanol (IPA, 8 mL) and water (8 mL), and the resulting mixtureheated to 65 to 70° C. to effect dissolution. The solution was cooled to45° C., and water (28 mL) was added over 15 minutes, maintaining thetemperature between 40 and 45° C. The slurry was cooled to 20 to 25° C.over an hour, then further cooled to 0 to 5° C. and held at thattemperature for 30 minutes before being filtered. The filter cake waswashed with 20% v/v IPA in water and the product dried under suctionovernight, then in vacuo (40° C.).

Example 3 Preparation of SelinexorSelinexorSelinexor Lot No. PG14-005(Form A)

The acetonitrile solvate of selinexor (Form D) was prepared inaccordance with the procedure described in Example 6.

The acetonitrile solvate of selinexor (1.07 Kg) was suspended in amixture of IPA (2.52 Kg) and water (3.2 Kg) and the mixture heated to 70to 75° C. to dissolve. The temperature was then adjusted to 40 to 45° C.and held at that temperature for 30 minutes. Water (10.7 Kg) was addedwhile maintaining the temperature at 40 to 45° C., then the batch wascooled to 20 to 25° C. and agitated at that temperature for 4 hoursbefore being further cooled to 0 to 5° C. After a further hour ofagitation, the slurry was filtered and the filter cake washed with acold mixture of IPA (0.84 Kg) and water (4.28 Kg) before being dried.

Example 4 Preparation of SelinexorSelinexorSelinexor Lot No. PC-14-009(Form A)

The acetonitrile solvate of selinexor (Form D) was prepared inaccordance with the procedure described in Example 6.

The acetonitrile solvate of selinexor (1.5 Kg) was suspended in IPA (3.6Kg) and water (4.5 Kg) and warmed to 37 to 42° C. with gentle agitation.The suspension was agitated at that temperature for 4 hours, and wasthen cooled to 1.5 to 20° C. over 1 hour. Water (15.1 Kg) was added,maintaining the temperature, then the agitation was continued for 1 hourand the batch was filtered. The filter cake was washed with a mixture ofIPA (1.2 Kg) and water (6 Kg), then dried under a flow of nitrogen.

Example 5 Preparation of Selinexor Lot Nos. 1339-BS-142-1, 1339-BS-142-2and PC-14-008 (Form A)

A reactor, under nitrogen, was charged with KG1 (1 Kg, 1.0 Eq), KJ8(0.439 Kg, 1.4 Eq) and MeTHF (7 L, 7 parts with respect to KG1).Diisopropylethylamine (0.902 Kg, 2.45 Eq with respect to KG1) was addedto the reaction mixture at −20° C. to −25° C. with a MeTHF rinse. To thereaction mixture, 50% T3P® in ethyl acetate (2.174 Kg, 1.2 Eq withrespect to KG1) was then charged, maintaining the temperature at −20° C.to −25° C. with a MeTHF rinse. After the completion of the addition, thereaction mixture was stirred briefly and then warmed to 20° C. to 25° C.Upon completion, the reaction mixture was washed first with water (5 L,5 parts with respect to KG1) and then with dilute brine (5 L, 5 partswith respect to KG1). The organic layer was concentrated by vacuumdistillation to a volume of 5 L (5 parts with respect to KG1), dilutedwith acetonitrile (15 L, 15 parts with respect to KG1) at approximately40° C. and concentrated again (5 L, 5 parts with respect to KG1). Aftersolvent exchange to acetonitrile, the reaction mixture was then heatedto approximately 60° C. to obtain a clear solution. The reaction mixturewas then cooled slowly to 0-5° C., held briefly and filtered. The filtercake was washed with cold acetonitrile (2 L, 5 parts with respect toKG1) and the filter cake was then dried under a stream of nitrogen toprovide the acetonitrile solvate of selinexor (Form D) as a slightlyoff-white solid.

Form D of selinexor (0.9 Kg) was suspended in IPA (2.1 Kg, 2.7L, 3 partswith respect to Form D) and water (2.7 Kg, 2.7 L, 3 parts with respectto Form D) and warmed to approximately 40° C. The resulting suspensionwas agitated for about 4 hours, selinexor, cooled to approximately 20°C., and diluted with additional water (9 Kg, 10 parts with respect toForm D). The mixture was stirred for a further 4-6 hours, then filtered,and the cake washed with a mixture of 20% IPA and water (4.5 L, 5 partswith respect to Form D). The filter cake was then dried under vacuum toprovide selinexor designated Lot No. PC-14-008 as a white crystallinepowder with a >99.5% a/a UPLC purity (a/a=area to area of all peaks;UPLC-ultra performance HPLC).

Example 6 Preparation of Selinexor Lot No. 1405463 (Form A)

Selinexor Lot No. 1405463 was prepared in accordance with the followingreaction scheme:

A reactor was charged with KG1 (15.8 Kg), (6.9 Kg) and MeTHF (90 Kg).Diisopropylethylamine (14.2 Kg) was added to the reaction mixture overapproximately 35 minutes at about −20° C. Following the addition of thediisopropylethylamine, T3P® (50% solution in EtAOc, 34.4 Kg) was addedmaintaining the temperature at −20° C. The mixture stirred to completethe reaction first at −20° C., then at ambient temperature.

Upon completion of the reaction, water (79 Kg) was added over about 1hour. The layers were separated and the organic layer was washed with amixture of water (55 Kg) and brine (18 Kg). The mixture was filtered,and the methyl-THF/ethyl acetate in the mixture distillatively replacedwith acetonitrile (volume of approximately 220 L). The mixture waswarmed to dissolve the solids, then slowly cooled to 0 to 5° C. beforebeing filtered. The filter cake was washed with acetonitrile to providethe acetonitrile solvate of selinexorSelinexorSelinexor (Form D).

The acetonitrile solvate of selinexorSelinexorSelinexor was dried, thenmixed with isopropanol (23 Kg) and water (55 Kg). The slurry was warmedto about 38° C. and held at that temperature for approximately 4 hoursbefore being cooled to 15 to 20 C. Water (182 Kg) was added. After afurther 5 hours of agitation, the mixture was filtered and the filtercake washed with a mixture of isopropanol (14 Kg) and water (73 Kg),before being dried under vacuum (45° C.). The dried product was packagedto provide selinexorSelinexorSelinexor Lot No. 1405463 (Form A).

Example 7 Polymorphism Studies of Selinexor

A comprehensive polymorphism assessment of selinexor was performed in arange of different solvents, solvent mixtures and under a number ofexperimental conditions based on the solubility of selinexor. Threeanhydrous polymorphs of selinexorSelinexorSelinexor were observed byXRPD investigation, designated Form A, Form B and Form C. Form A is ahighly crystalline, high-melting form, having a melting point of 177°C., and was observed to be stable from a physico-chemical point of viewwhen exposed for 4 weeks to 25° C./97% relative humidity (RH) and to 40°C./75% RH. A solvated form of selinexor was also observed inacetonitrile, designated Form D. A competitive slurry experimentconfirmed Form A as the stable anhydrous form under the conditionsinvestigated, except in acetonitrile, in which solvate formation wasobserved. It was further found that in acetonitrile, below 50° C., onlyForm D is observed, at 50° C. both Form A and Form D are observed, andat 55° C., Form A is observed.

General Instrument and Methodology Details X-Ray Powder Diffraction(XRPD)

Bruker AXS C2 GADDS: XRPD patterns were collected on a Bruker AXS C2GADDS diffractometer using Cu Kα radiation (40 kV, 40 mA), automated XYZstage, laser video microscope for auto-sample positioning and a HiStar2-dimensional area detector, X-ray optics consists of a single Gobelmultilayer mirror coupled with a pinhole collimator of 0.3 mm. A weeklyperformance check is carried out using a certified standard NIST 1976Corundum (flat plate).

The beam divergence, i.e., the effective size of the X-ray beam on thesample, was approximately 4 mm. A θ-θ continuous scan mode was employedwith a sample detector distance of 20 cm which gives an effective 2θrange of 3.2°-29.7°. Typically, the sample would be exposed to the X-raybeam for 120 seconds. The software used for data collection was GADDSfor XP/2000 4.1.43 and the data were analyzed and presented usingDiffrac Plus EVA v13.0.0.2 or v15.0.0.0.

Ambient conditions. Samples run under ambient conditions were preparedas flat plate specimens using powder as received without grinding.Approximately 1-2 mg of the sample was lightly pressed on a glass slideto obtain a flat surface.

Non-ambient conditions. Samples run under non-ambient conditions weremounted on a silicon wafer with heat-conducting element. The sample wasthen heated to the appropriate temperature and data collection wasinitiated.

Bruker AXS D8 Advance. XRPD patterns were collected on a Bruker D8diffractometer using Cu Kα radiation (40 kV, 40 mA), θ-2θ goniometer,and divergence of V4 and receiving slits, a Ge monochromator and aLynxeye detector. The instrument is performance checked using acertified Corundum standard (NIST 1976). The software used for datacollection was Diffrac Plus XRD Commander v2.6.1 and the data wereanalyzed and presented using Diffrac Plus EVA v13.0.0.2 or v15.0.0.0.

Samples were run under ambient conditions as flat plate specimens usingpowder as received. The sample was gently packed into a cavity cut intopolished, zero-background (510) silicon wafer. The sample was rotated inits own plane during analysis. The details of the data collection are:

-   Angular range: 2 to 42 °2θ-   Step size: 0.05 °2θ-   Collection time: 0.5 s/step.

Differential Scanning Calorimetry (DSC)

Mettler DSC 823e. DSC data were collected on a Mettler DSC 823E equippedwith a 34 position auto-sampler. The instrument was calibrated forenergy and temperature using certified indium. Typically, 0.5-3 mg ofeach sample, in a pin-holed aluminum pan, was heated at 10° C./minutefrom 25° C. to 300° C. A nitrogen purge at 50 ml/min was maintained overthe sample. The instrument control and data analysis software was STARev9.20.

Thermogravimetric Analysis (TGA)

Mettler TGA/SDTA. 851e. TGA data were collected on a Mettler TGA/SDTA851e equipped with a 34 position auto-sampler. The instrument wastemperature calibrated using certified indium. 5-30 mg of each samplewas loaded onto a pre-weighed aluminum crucible and was heated at 10°C./min from ambient temperature to 350° C. A nitrogen purge at 50 ml/minwas maintained over the sample. The instrument control and data analysissoftware was STARe v9.20.

Chemical Purity Determination by High Performance Liquid Chromatography(HPLC)

Purity analysis was performed on an Agilent HP1100 series systemequipped with a diode array detector using ChemStation softwarevB.02.01-SR1 (or SR2) as follows:

Sample Preparation ~0.4 mg/mL in 1:1 ACN/MeOH Column Zorbax SB-Phenyl,4.6 mm × 150 mm, 5 μm Column Temperature (° C.) 40 Injection (μl) 10Detection: 245 nm Wavelength, Bandwidth (nm) Flow Rate (ml/min) 1.0Phase A 20 mM Ammonium Acetate in Water (no pH specified) Phase B 70%ACN, 30% MeOH (v/v)

Solubility Assessment

The polymorphism assessment of selinexorSelinexorSelinexor included asolubility assessment, performed on Form A. About 20 mg ofselinexorSelinexorSelinexor prepared in accordance with the processdescribed in Example 1 were weighed into vials and the solubility wasvisually assessed at 50° C. in thirty solvents and solvent mixtures.After each solvent addition, samples were kept under agitation for 10minutes at 50° C. before each assessment; the samples were then left tocool to room temperature (RT) before a second assessment was done at RT.Samples/conditions that fully dissolved were designated as soluble,samples that showed signs of partial solubility (thinning, notably lesssolids) were designated partially soluble (PS) and conditions lackingvisual signs of solubility were designated insoluble. Three furthersolubility assessments were performed in IPA:water, MeCN:water andMTBE:heptane mixtures. Note: solubility was assessed within 5 and 100volumes for the different solvents used. 1 volume (in μL) is equal tothe sample mg used in the assessment (e.g., for a 20 mg sample, 1 volumeis equal to 20 μL).

Selinexor was soluble in about 60% of the solvent systems investigated.selinexor was not soluble in heptane, 1,2-dichloroethane, toluene,cumene, diisopropylether, water and isooctane. selinexor was alsosoluble in IPA:water mixtures having up to 60% water and inacetonitrile:water mixtures having up to 40% water in the mixture.Selinexor was not found to be soluble in up to 100 volumes of any of theMTBE:heptane mixtures investigated.

Polymorphism Studies

The samples produced during the solubility assessment were processed asfollows:

-   The clear solutions obtained were placed for cooling at 5° C. and    eventually at −20° C. for at least 12 hours (−20° C. step was    introduced or not depending on the 5° C. result and on the nature of    the solvent). Samples found as clear solutions at −20° C. were    placed for slow evaporation at RT using a 25-gauge syringe needle(s)    in the septum of the vial.-   The solid suspensions observed in 100 volumes of the relative    solvent systems at the end of the solubility assessment were placed    for 8 days maturation cycling between RT and 50° C. (cycling 4 hours    at RT; 4 hours at 50° C.). After the maturation, the solids were    recovered and the supernatants were placed for slow evaporation.-   All the solids obtained by these processes were analyzed by XRPD.

Most of the solids recovered from the processed samples were found to beconsistent with Form A (76% of the hits). The X-ray diffractogram ofForm A is depicted in FIG. 1A. Representative XRPD peaks for Form Adepicted in FIG. 1A are as follows:

2-Theta ° Intensity % 4.4 50.9 12.4 19.9 13.1 23.3 14.5 8.7 14.7 13.115.8 23.6 16.9 8.0 17.5 7.9 18.2 22.2 19.9 100.0 20.3 47.0 21.3 85.622.0 58.1 23.1 16.2 23.5 43.1 23.7 37.5 23.9 13.6 25.0 44.8 25.3 10.025.6 13.6 27.0 21.4 27.3 11.5 28.3 28.6 28.5 31.3 31.4 19.1 34.8 11.337.2 13.6

The starting anhydrous form (Form A) was found to be stable in most ofthe conditions investigated and no other anhydrous forms were observedto form under solvent-based experimentation. By TGA, 0.4% w/w of weightloss was observed between 160° C. and 200° C. DSC analysis showed anendothermic event at 177° C. due to the melting of the sample (meltingwas also observed by VT-XRPD). The DSC and TGA thermograms of Form A aredepicted in FIG. 1B.

In MeCN, Form A was observed to convert to a solvated form, designatedForm D (MeCN solvate). The X-ray powder diffractogram of Form D isdepicted in FIG. 2A. Representative XRPD peaks for Form D depicted inFIG. 2A are as follows:

2-Theta ° Intensity % 3.7 51.7 7.3 85.9 9.7 40.8 10.9 32.5 11.1 24.613.1 8.8 18.3 38.0 19.2 35.5 19.5 23.7 20.4 6.0 20.6 21.2 21.9 100.022.3 9.8 22.5 9.7 23.9 5.3 24.4 7.4 26.8 6.8 28.9 8.4 29.3 3.7 29.5 3.430.1 3.8 31.9 7.1 32.5 3.1 33.1 3.1 33.7 4.4 38.1 3.0 41.3 4.1

Form D was also observed in acetonitrile-water mixtures of greater than20% v/v MeCN in water. In 20% v/v MeCN in water, the solid recovered wasconsistent with Form A by XRPD. These results are described in Table 1.

TABLE 1 Solvent Solubility Experiment Result XRPD Acetonitrile Dissolvedin 70v Placed at −20° C. Solid Form D recovered 90% MeCN:10% WaterDissolved in 30v Placed at 5° C. Solid Form D recovered 80% MeCN:20%Water Dissolved in 30v Placed at 5° C. Solid Form D recovered 60%MeCN:40% Water Dissolved in 50v Placed at 5° C. Solid Form D recovered40% MeCN:60% Water Not dissolved Placed for slow evap. Solid Form D in100v recovered 20% MeCN:80% Water Not dissolved Matured 25° C.-50° C.Solid Form A in 100v recovered

TGA and DSC were performed on a sample of Form D. By TGA, a weight lossof 1.86% w/w was observed due to acetonitrile loss. However, the weightloss by TGA was influenced by preparation timing of the sample after therecovery of the material. By DSC, an endothermic/exothermic event wasobserved around 152° C. followed by an endothermic event at 177° C. TheDSC behavior is similar to that observed for Form C (see below).

A sample of selinexor isolated from MeCN was analyzed by DSC and TGAimmediately after recovering from the solvent. A solvent loss of 6.5%w/w was observed by TGA. DSC showed an endothermic event around 77° C.due to the solvent loss followed by endo/exo event of Form C and meltingevent of Form A around 178° C. The DSC and TGA thermograms of Form D aredepicted in FIG. 2B. As mentioned above, the solvent loss observed byTGA can vary depending on the time the sample of Form D is exposed toambient conditions during sample preparation. A solvent loss of 6.5%corresponds to 0.75 moles of solvent per mole of compound. Although notwishing to be bound by any particular theory, Form D could be amono-solvate which can be desolvated with drying and is thus sensitiveto isolation conditions, as evidenced by the TGA results.

Form D was analyzed by VT-XRPD, Form D was observed to convert to Form Cafter 80° C. and Form C was observed to convert to Form A. On heating,after the release of the solvent, solvate Form D converts to Form C; theendo/exo event observed by DSC around 152° C. is due to Form C.Moreover, Form D was dried for 15 hours at 80° C. and 3 mbar. The samplerecovered was found as consistent with Form C. By HPLC and ¹H-NMRanalysis, the recovered sample (Form C) was found as 99.6% pure, asmeasured by the area under the curve (AUC), and the compound structurewas confirmed. No residual MeCN was observed after heating and dryingthe sample.

Drying and thermal experiments (including VT-XRPD analysis) were foundto convert Form D to anhydrous Forms B and C. The thermal transition ofForm B to Form C and the thermal transition of Form C to Form A wereobserved by DSC. Form D was also observed to convert to Form A uponheating and upon maturation of Form D in a 20% volume/volume (v/v) MeCNin water mixture. However, mixtures of acetonitrile:water withacetonitrile above 20% v/v have shown a conversion of the anhydrouscompound to the acetonitrile solvate (Form D).

Form B was analyzed by DSC, TGA, XRPD and variable temperature XRPD(VT-XPRD). The X-ray powder diffractogram for Form B is depicted in FIG.3A. Representative XRPD peaks for Form B depicted in FIG. 3A are asfollows:

2-Theta ° Intensity % 8.1 26.1 9.4 100.0 11.1 60.7 13.8 23.3 16.5 50.218.3 51.9 18.8 59.8 20.2 47.3 20.8 45.3

The DSC and TGA thermograms are depicted in FIG. 3B. This polymorphshows an endothermic melting around 91° C. immediately followed by arecrystallization exotherm. A second endo/exo event is observed around155° C. followed by an endothermic event at 179° C. By VT-XRPD analysis,it is possible to explain the events observed by DSC: the first endo/exoevent at 91° C. is the melting of Form B followed by recrystallizationtowards Form C; the latter melts around 155° C. and recrystallizestowards Form A, which melts at 179° C. By TGA, no relevant weight losseswere observed.

Form C was analyzed by DSC, TGA and XRPD. The X-ray powder diffractogramfor Form C is depicted in FIG. 4A. Representative XRPD peaks for Form Cdepicted in FIG. 4A are as follows:

2-Theta ° Intensity % 3.7 100.0 10.5 9.0 11.2 15.4 12.1 15.0 14.9 7.416.4 8.6 17.7 13.3 18.6 32.0 19.7 15.4 21.2 14.2 22.2 15.2

The DSC and TGA thermograms are depicted in FIG. 4B. By DSC, anendothermic/exothermic event was observed around 155° C., followed by anendothermic event around 179° C. No VT-XRPD analysis was perforated forthis form but, as per the Form B VT-XRPD experiment, the endo/exo eventis due to the melting/recrystallisation of Form C towards Form A; thesecond endothermic event at 179° C. is the melting of Form A. Nosignificant weight losses were observed by TGA analysis for Form C.

Competitive Slurry Experiment

In a competitive slurry experiment, approximately 1:1 mixtures of Form Aand Form C were prepared. The mixtures were then stirred with a seed ofForm B (about 5% by weight of the mixture of Form A and Form C). Theproduced systems were matured at 5° C., 25° C. and 50° C. under stirringin acetonitrile, ethyl acetate, 30% IPA:70% water and 30% ethanol:70%water. The systems were analyzed by XRPD at two different time points:72 hours and 1 week.

Mixtures matured in ethyl acetate, IPA:water and ethanol:water werefound to be consistent with Form A by XRPD. Form D was observed bymaturation in acetonitrile. This experiment confirmed Form A as thestable form, compared to Form B and Form C, under the conditionsinvestigated. The presence of acetonitrile led to solvate formation atall of the temperatures investigated.

Example 8 Particle Size Method and Sample Analyses

A particle size method for analysis of samples of selinexor wasdeveloped, and a number of samples of selinexor were analyzed using thedeveloped method.

General Instrument and Methodology Details Polarized Light Microscopy

Polarized light microscopy was performed using a Leica DM LP microscopeequipped with a Spot Insight color camera. Crossed-polarized light wasused with a first order red compensator. Various objectives were used toview the sample. Images were acquired at ambient temperature using SpotAdvanced software (v.4.5.9). Micron bars were added to the images tohelp estimate particle sizing.

Particle Size Analysis

Particle size data was acquired using a Malvern Instruments MS2000equipped with a Hydro2000 μP dispersion unit. Data was collected andanalyzed using Mastersizer 2000 v 5.60 software, using volume-basedmeasurements. NIST-traceable glass beads were used as the referencestandard.

Polarized Light Microscopy

Photomicrographs of selinexor Lot No. 1305365 (described in Example 1)dispersed in mineral oil were collected to determine the morphology ofthe sample to determine if the sample was agglomerated and to gain aninitial estimate of the size of the particles. Based both uponobservation during analysis and on the obtained images, the sample wascomposed primarily of blades and acicular particles 10-100 μm in length,some smaller, irregularly-shaped particles and some agglomerates 200-300μm in length that dispersed easily in mineral oil. Later observations ofthe sample suspended in various dispersants showed larger agglomerates,but these were also easily dispersed. Table 2 summarizes theseobservations.

TABLE 2 Polarized Light Microscopy of selinexor Lot No. 1305365.Suspension medium Observations^(a) Mineral oil Blades and acicularparticles 10-100 μm, some smaller, irregularly-shaped particles and someagglomerates 200-300 μm. 0.1% (w/v) Blades and acicular particles 10-100μm, some Lecithin in smaller, irregularly-shaped particles and someIsopar G very large agglomerates. 0.3% (w/v) Span Blades and acicularparticles 10-100 μm, some 85 in heptane smaller, irregularly-shapedparticles and some larger agglomerates. 0.1% (w/v) Tween Blades andacicular particles 10-100 μm, some 20 in water smaller,irregularly-shaped particles and some larger agglomerates.^(a)Observations recorded during microscopy and based upon resultingphotomicrographs

Particle Size Method and Sample Analyses

Details of the method conditions used to analyze particle size arelisted below

Sample refractive index: 1.596

Sample absorption: 0.001

Dispersant: 0.1% (w/v) Lecithin in Isopar G

Dispersant refractive index: 1.42

Pump speed: 2100 rpm

Recirculation time: 2 minutes

Sample measurement time: 30 sec.

Background measurement time: 30 sec.

Model: general purpose

Sensitivity: normal

Particle shape: irregular.

The relative standard deviations for the d10, d50 and d90 using thesemethod conditions were 2.35%. 1.28% and 6.17%, respectively. Alldeviations fall well within the USP recommendation of ≤30%, ≤10%, ≤15%for the d10, d50 and d90, respectively.

One particle size measurement of each lot of selinexor described inExamples 1-6 was collected using the particle size method conditionsdescribed above. Table 4 provides sample information and particle sizeanalysis data for the indicated lot of selinexor under the particle sizemethod analysis conditions described above. FIGS. 5A-5P are particlesize distribution graphs and show the particle size distribution of thesample corresponding to the indicated lot of selinexor under theparticle size method analysis conditions described above.

TABLE 4 Sample Information and Particle Size Analysis of Selinexor Usingthe Revised Method Conditions. D[4,3] Volume d10 d50 d90 weightedCorresponding Lot No. (μm)^(a) (μm)^(b) (μm)^(c) mean FIG. No. 3053654.905 14.268 75.098 34.740 FIG. 5A & FIG. 5B 1341-AK- 2.815 10.42389.782 32.779 FIG. 5C & FIG. 5D 109-2 PC-14-005 6.363 18.345 86.90636.720 FIG. 5E & FIG. 5F 1339-BS- 4.978 11.969 28.331 16.614 FIG. 5G &FIG. 5H 142-1 1339-BS- 6.088 14.468 33.936 20.889 FIG. 5I & FIG. 5J142-2 PC-14-008 7.658 18.290 42.616 22.451 FIG. 5K & FIG. 5L PC-14-0099.665 24.406 68.445 32.557 FIG. 5M & FIG. 5N 1405463 12.062 27.65364.876 33.740 FIG. 5O & FIG. 5P ^(a)10% of the total volume of particlesis comprised of particles no larger than the indicated size. ^(b)50% ofthe total volume of particles is comprised of particles no larger thanthe indicated size. ^(c)90% of the total volume of particles iscomprised of particles no larger than the indicated size.

Particle size distributions for measurements obtained from the samplescorresponding to the lots of selinexor described in Examples 1-5 werealso overlaid in a graph. FIG. 5I is the graph resulting from overlayingthe particle size distributions obtained from the samples correspondingto the lots of selinexor described in Examples 1-5, and shows that thedistributions for four lots (Lot Nos. 1339-BS-142-1, 1339-BS-142-2,PC-14-008 and PC-14-009) had more a a unimodal characteristic than lot(Lot No. 1341-AK-109-2) which was distinctly bimodal. The distributionfor Lot No. PC-14-005, like the sample used for method development (LotNo. 1305365), showed a primary mode approximately 12-15 um, a secondarymode consisting or a shoulder on the side of the primary modecorresponding to particles approximately 80-90 μm and a minor tertiarymode approximately 400 μm.

Example 9 Process for Preparation of SelinexorSelinexorSelinexor

A new process for the preparation of the compound of Formula I wasdeveloped. The new process uses a T3P®-mediated coupling of KG1 with thehydrazinyl pyrazine (KJ8) to prepare selinexor such as described ininternational Publication No. WO 2013/019548, but incorporates changesto the reaction design. In particular, the process described hereinwherein the unstable active T3P® ester is generated in situ can resultin one or more of the following advantages over the process for thepreparation of the compound of Formula I described in internationalPublication No. WO 2013/019548:

-   -   A reduction of the isomerization of the double bond in KH8:    -   A reduction in the production of side products such as        3-(3,5-Bis-trifluoromethyl-phenyl)-1H-[1,2,4]triazole (KF9);    -   An increase in purity; and    -   An increase in yield.        The process described herein has been implemented into        multi-kilogram processes, see for example the 1 Kg scale process        (see Example 5), providing the desired final product in about        80% yield overall. Details of the improved process for preparing        selinexor are described in Examples 5 and 6.

Chemistry Development

A series of experiments aimed at generating the activated estertransiently were carried out. These experiments are summarized in Table5, Initially, the addition mode of the raw materials into T3P® wasmaintained, but subsequently the order of addition of the raw materialswas reversed when it was observed that the kinetics of the reaction ofKJ8 with T3P® were overshadowed by the rate of formation (and presumablyquench) of the T3P® ester of KG1. In addition to the improvements in thecharacteristics of the product of the coupling between KG1 and KJ8discussed above, the addition of T3P® to a mixture of KG1, KJ8 and DIPEAprovided a greatly simplified reactor flow and the need to verycarefully handle the T3P® activated ester of KG1 once formed. Adding theT3P® last also allowed for much better control over the addition rates,avoiding the need to transfer the activated ester as rapidly as possible(a task that is much more difficult and potentially dangerous at largerscale). Reduction in the number of solutions that had to be prepared andtransferred also allowed us to reduce the volume of the process.

TABLE 5 Conv. KF9 Temp Eq. Eq. Eq. Type/Eq. Type/Eq. (% KH8/(% KG1 +Cis:Trans (wrt Solvent ° C. T3P KG1 KJ8 Base Base #2 Addn. order IPCtime KH8))*100 ratio KH8) MeTHF/ −40 to 1.4 1.00 1.1 DIPEA NAKG1/DIPEA/MeTHF −40° C. 1 h 95.4% 98.4%  5.3% EtOAc −45 2.45 cool −40°C. RT O/N 97.4% 98.4%  5.4% Charge T3P Add to KJ8 (−40° C.) MeTHF/ −40to 1.4 1.00 1.1 DIPEA NA T3P/MeTHF −40° C. 1 h 84.8% >99% 2.7% EtOAc −452.45 cool −40° C. RT O/N 93.8% >99% 2.7% KG1/KJ8/DIPEA MeTHF/ −40 to 1.41.00 1.1 DIPEA DIPEA T3P/MeTHF −40° C. 1 h 81.2% >99% 2.95%  EtOAc −451.0  1.45 cool −40° C. −40° C. 3 h 81.4% >99% 3.0% KG1/KJ8/DIPEA (1) RTWE DIPEA (1.45) MeTHF/ −40 to 1.4 1.00 1.1 DIPEA DIPEA T3P/MeTHF −40° C.1 h 77.7% 98.9%  4.0% EtOAc −45 1.0  1.45 Cool −40° C. −40 3 h 90.3%99.2%  3.6% Charge DIPEA (1.4) KG1/KJ8/DIPEA (1) MeTHF/ −40 to 1.4 1.001.1 DIPEA DIPEA T3P/KJ8 −40° C. 30 m 45.0% >99% 4.1% EtOAc −45 1.0  1.45cool −40° C. RT 1 h 63.5% >99% 3.65%  DIPEA (1.45) KG1/DIPEA (1) MeTHF/−40 to 1.4 1.00 1.1 DIPEA NA T3P/KJ8 −40° C. 30 m 13.4% >99% 12.0% EtOAc −45 2.45 Cool −40° C. RT 1 h 28.1% 98.5%  DIPEA Charge KG1/MeTHFMeTHF/ −40 to 1.4 1.00 1.1 DIPEA NA Charge T3P/KJ8 −40° C. 30 m69.1% >99% 2.8% EtOAc −45 2.0  Cool −40° C. RT 1 h 70.9% >99% 2.7%Charge KG1/DIPEA MeTHF/ −40 to 1.4 1.00 1.25 DIPEA NA T3P/MeTHF −40° C.90 m 88.3% >99% 2.34%  EtOAc −45 2.45 Cool −40° C. RT 1 h 96.4% >99%2.25%  KG1/KJ8/DIPEA (1) MeTHF/ −40 to 1.4 1.00 1.1 DIPEA NAKG1/MeTHF/DIPEA −40° C. 30 m 97.2%  99% 3.8% EtOAc −45 2.45 Cool −40° C.RT 30 m 98.5% 98.6%  4.3% T3P KJ8/MeTHF MeTHF/ −40 to 1.4 1.00 1.1 DIPEANA KG1/KJ8/MeTHF −40° C. 90 m 97.1% 98.9%  2.1% EtOAc −45 2.45 DIPEA RT1 h 98.0% 98.7%  2.1% Cool −40° C. T3P Notes: KF9 =3-(3,5-Bis-trifluoromethyl-phenyl)-1H-[1,2,4]triazole. Column labeledTemp ° C. reflect a range of acceptable conditions, while thetemperature listed in the column Addn. order reflects the actualtemperature.

Investigation of reaction temperature is summarized in Table 6. Ofparticular note was the observation that the new chemistry did notrequire the use of cryogenic vessels, as the reaction could be run at−20° C. or even −10° C. with only a slight increase in the level of KF9produced. In some embodiments, the stoichiometry based on 1.00equivalent of KG1 is 1.05 equivalents of KJ8 and 1.6 equivalents of T3P.It is understood that the equivalents of KJ8, T3P or both can vary by±5% without impact on purity and yield. In some embodiments, thereaction is run at −20° C. In some embodiments, the reaction is run at−40° C.

TABLE 6 Conv. KF9 Eq. Eq. Eq. Type/Eq. (% KH8/(% KG1 + Cis:Trans (wrtSolvent Temp T3P KG1 KJ8 Base Addn. order IPC time KH8))*100 ratio KH8)MeTHF/ −40 to 1.4 1.00 1.1 2.45 KG1/KJ8/MeTHF −40° C. 90 m 97.2% >99.9%0.48% EtOAc −45 New DIPEA RT 2.5 h 98.2% >99.9% 0.71% Cool −40° C., T3PMeTHF/ −20 to 1.4 1.00 1.1 2.45 KG1/KJ8/MeTHF −20° C. 40 m 96.6% >99.9%0.91% EtOAc −25 New DIPEA RT 1 h 97.6% >99.9% 0 96% Cool −20° C.; T3PMeTHF/ −40 to 1.6 1.00 1.1 DIPEA KG1/KJ8/MeTHF −40° C. 5 m 91.9% >99.9%0 70% EtOAc −45 New 2.45 DIPEA RT 1 h 99.0% >99.9% 0.90% Cool −40° C..T3P MeTHF/ −40 to 1.4 1.00 1.4 DIPEA KG1/KJ8/MeTHF −40° C. 10 m98.2% >99.9% 0.77% EtOAc −45 New 2.45 DIPEA RT 1 h 99.4% >99.9% 0 86%Cool −40° C.; T3P MeTHF/ −40 to 1.4 1.00 1.4 DIPEA KG1/KJ8/MeTHF −40° C.10 m 98.5% >99.9% 0.90% EtOAc −45 New 2.45 DIPEA RT 1 h  99% Cool −40°C.; T3P MeTHF/ −10 to 1.4 1.00 1.4 DIPEA KG1/KJ8/MeTHF −10° C. 10 m 100% >99.9% 1.30% EtOAc −15 New 2.45 DIPEA RT 1 h  100% >99.9% Cool−10° C.; T3P Note: Column labeled Temp ° C. reflect a range ofacceptable conditions, while the temperature listed in the column Addn.order reflects the actual temperature.

The teachings of all patents, published applications and referencescited herein are incorporated by reference in their entirety.

While this invention has been particularly shown and described withreferences to example embodiments thereof, it will be understood bythose skilled in the art that various changes in form and details may bemade therein without departing from the scope of the inventionencompassed by the appended claims.

What is claimed is:
 1. A composition, comprising particles of a singlecrystalline form of a compound represented by Structural Formula I:

wherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9°, 21.3° and 22.0° ; and the particles have a unimodalparticle size distribution characterized by a d(0.9) of 100 microns orless.
 2. The composition of claim 1, where the a unimodal particle sizedistribution characterized by a d(0.9) of 70 microns or less.
 3. Thecomposition of claim 1 or 2, wherein the crystalline form ischaracterized by X-ray powder diffraction peaks at 2θ angles of 4.4°,19.9°, 21.3° and 22.0°.
 4. The composition of claim 3, wherein thecrystalline form is characterized by X-ray powder diffraction peaks at2θ angles of 4.4°, 19.9°, 20.3°, 21.3°, 22.0°, 23.5° and 25.0°.
 5. Thecomposition of claim 4, wherein the crystalline form is characterized byX-ray powder diffraction peaks at 2θ angles of 4.4°, 13.1°, 15.8°,18.2°, 19.9°, 20.3°, 21.3°, 22.0°, 23.5°, 23.7°, 25.0°, 27.0°, 28.3° and28.5°.
 6. The composition of claim 1 or 2, wherein the crystalline formis characterized by an X-ray powder diffraction pattern substantially inaccordance with that depicted in FIG. 1A.
 7. The composition of any oneof claims 1-6, wherein the crystalline form is further characterized bya differential scanning calorimetry thermogram comprising an endothermicpeak at 179° C.
 8. The compositions of any one of claims 1-7, whereinthe particles have a particle size distribution characterized by ad(0.9) of from 10 microns to 100 microns.
 9. The composition of claim 8,wherein the particles have a particle size distribution characterized bya d(0.9) of from 25 microns to 100 microns.
 10. The composition of claim9 wherein the particles have a particle size distribution characterizedby a d(0.9) of from 60 microns to 100 microns.
 11. The compositions of9, wherein the particles have a particle size distribution characterizedby a d(0.9) of from 10 microns to 70 microns.
 12. The composition ofclaim 9, wherein the particles have a particle size distributioncharacterized by a d(0.9) of from 25 microns to 70 microns.
 13. Thecomposition of claim 9, wherein the particles have a particle sizedistribution characterized by a d(0.9) of from 60 microns to 70 microns.14. The composition of any one of claims 1-13, wherein the particleshave a particle size distribution characterized by a d(0.5) of from 10microns to 35 microns.
 15. The composition of claim 14, wherein theparticles have a particle size distribution characterized by a d(0.5) offrom 15 microns to 30 microns.
 16. The composition of claim 15, whereinthe particles have a particle size distribution characterized by ad(0.5) of from 25 to 30 microns,
 17. The composition of any one ofclaims 1-16, wherein the particles have a particle size distributioncharacterized by a d(0.1) of 5 microns or greater.
 18. The compositionof claim 17, wherein the particles have a particle size distributioncharacterized by a d(0.1) of greater than 10 microns.
 19. Thecomposition of any one of claims 1-17, wherein the particles leave aparticle size distribution characterized by a d(0.1) of from 5 micronsto 15 microns.
 20. The composition of claim 19, wherein the particleshave a particle size distribution characterized by a d(0.1) of from 10microns to 15 microns.
 21. The composition of any one of claims 1-20,wherein the particles have a normal particle size distribution.
 22. Thecomposition of any one of claims 1-21, wherein the particles have aparticle size distribution characterized by a d(0.9):d(0.1) ratio of 10or less.
 23. The composition of claim 22, characterized by ad(0.9):d(0.1) ratio of 7.5 or less.
 24. The composition of claim 23,characterized by a d(0.9):d(0.1) ratio of 6 or less.
 25. The compositionof any one of claims 1-21, wherein the particles have a particle sizedistribution characterized by a d(0.9):d(0.1) ratio of from 2 to
 10. 26.The composition of claim 25, characterized by a d(0.9):d(0.1) ratio offrom 5 to 7.5.
 27. The composition of claim 26, characterized by ad(0.9):d(0.1) ratio of from 5 to
 6. 28. The composition of any one ofclaims 1-27, wherein the particles have a particle size distributioncharacterized by a d(0.9):d(0.5) ratio of 4.5 or less.
 29. Thecomposition of claim 28, characterized by a d(0.9):d(0.5) ratio of 3 orless.
 30. The composition of any one of claims 1-29, wherein theparticles have a particle size distribution characterized by ad(0.9):d(0.5) ratio of from 1.5 to 4.5.
 31. The composition of claim 30,characterized by a d(0.9):d(0.5) ratio of from 2 to
 3. 32. Apharmaceutical composition, comprising a composition of any one ofclaims 1-31 and a pharmaceutically acceptable carrier.
 33. A method fortreating a disorder associated with CRM1 activity, the method comprisingadministering to a subject in need thereof a therapeutically effectiveamount of a pharmaceutical composition of claim
 32. 34. The method ofclaim 33, wherein the disorder is a proliferative disorder, cancer, aninflammatory disorder, an autoimmune disorder, a viral infection, anophthalmological disorder, a neurodegenerative disorder, a disorder ofabnormal tissue growth, a disorder related to food intake, an allergicdisorder, or a respiratory disorder.
 35. The method of claim 34, whereinthe disorder is cancer.
 36. A method for promoting wound healing in asubject in need thereof, comprising administering to the subject in needthereof a therapeutically effective amount of a pharmaceuticalcomposition of claim
 32. 37. A single crystalline form of a compoundrepresented by Structural Formula I:

wherein the single crystalline form is Form D and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from3.7°, 7.3°, 10.9°, 18.3° and 21.9°.
 38. The single crystalline form ofclaim 37, characterized by X-ray powder diffraction peaks at 2θ anglesof 3.7°, 7.3°, 10.9°, 18.3° and 21.9°.
 39. The single crystalline formof claim 38, characterized by X-ray powder diffraction peaks at 2θangles of 3.7°, 7.3°, 9.7°, 10.9°, 18.3°, 19.2° and 21.9°.
 40. Thesingle crystalline form of claim 39, characterized by X-ray powderdiffraction peaks at 2θ angles of 3.7°, 7.3°, 9.7°, 10.9°, 11.1°, 18.3°,19.2°, 19.5°, 20.6° and 21.9°.
 41. The single crystalline form of claim37, characterized by an X-ray powder diffraction pattern substantiallyin accordance with that depicted in FIG. 2A.
 42. The single crystallineform of any one of claims 37-41, wherein the compound of StructuralFormula I is in the form of a solvate.
 43. The single crystalline formof claim 42, wherein the solvate is an acetonitrile solvate,
 44. Thesingle crystalline form of claim 42 or 43, wherein the solvate comprisesfrom about 0.5 to about 1.5 molar equivalents of solute per molarequivalent of the compound of Structural Formula I.
 45. The singlecrystalline form of claim 44, wherein the solvate comprises about onemolar equivalent of solute per molar equivalent of the compound ofStructural Formula I.
 46. A single crystalline form of a compoundrepresented by Structural Formula I:

wherein the single crystalline form is Form C and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from3.7°, 11.2°, 12.1° and 18.6°.
 47. The single crystalline form of claim46, characterized by X-ray powder diffraction peaks at 2θ anglesselected from 3.7°, 11.2°, 12.1° and 18.6°.
 48. The single crystallineform of claim 47, characterized by X-ray powder diffraction peaks at 2θangles selected from 3.7°, 11.2°, 17.7°, 12.1°, 18.6°, 19.7°, 21.2° and22.2°.
 49. The single crystalline form of claim 46, characterized by anX-ray powder diffraction pattern substantially in accordance with thatdepicted in FIG. 4A.
 50. A single crystalline form of a compoundrepresented by Structural Formula I:

wherein the single crystalline form is Form B and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from9.4°, 11.1°, 16.5°, 18.3° and 18.8°.
 51. The single crystalline form ofclaim 50, characterized by X-ray powder diffraction peaks at 2θ anglesof 9.4°, 11.1°, 16.5°, 18.3° and 18.8°.
 52. The single crystalline formof claim 51, characterized by X-ray powder diffraction peaks at 2θangles of 9.4°, 11.1°, 16.5°, 18.3°, 18.8°, 20.2° and 20.8°.
 53. Thesingle crystalline form of claim 52, characterized by X-ray powderdiffraction peaks at 2θ angles of 8.1°, 9.4°, 11.1°, 13.8°, 16.5°,18.3°, 18.8°, 20.2° and 20.8°.
 54. The single crystalline form of claim50, characterized by an X-ray powder diffraction pattern substantiallyin accordance with that depicted in FIG. 3A.
 55. A method of preparing asingle crystalline form of a compound represented by Structural FormulaI:

wherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9′, 21.3° and 22.0°. the method comprising: (a) suspendingsingle crystalline Form B, C or D of the compound of Structural FormulaI, or a mixture comprising two or more of single crystalline Form B, Cor D of the compound of Structural Formula I, in isopropanol or amixture of isopropanol and water to form a slurry, wherein singlecrystalline Form D is characterized by at least three X-ray powderdiffraction peaks at 2θ angles selected from 3.7°, 7.3°, 10.9°, 18.3°and 21.9°, single crystalline Form B is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 9.4°, 11.1°,16.5°, 18.3° and 18.8° and single crystalline Form C is characterized byat least three X-ray diffraction peaks at 2θ angles selected from 3.7°,11.2°, 12.1° and 18.6°; (b) heating the slurry to a temperature lessthan or equal to about 70° C. to form a second slurry or a solution; (c)cooling the second slurry or the solution and adding water to the secondslurry or the solution, thereby forming solid particles of crystallineForm A of the compound of Structural Formula I; and (d) isolating thesolid particles of crystalline Form A, thereby preparing a compositioncomprising particles of single crystalline Form A of the compound ofStructural Formula I.
 56. A method of preparing a single crystallineform of a compound represented by Structural Formula I:

wherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9°, 21.3° and 22.0°, the method comprising: (a) suspendingsingle crystalline Form B, C or D of the compound of Structural FormulaI, or a mixture comprising two or more of single crystalline B, C or Dof the compound of Structural Formula I, in isopropanol or a mixture ofisopropanol and water to form a slurry, wherein single crystalline FormD is characterized by at least three X-ray powder diffraction peaks at2θ angles selected from 3.7°, 7.3°, 10.9°, 18.3° and 21.9°, singlecrystalline Form B is characterized by at least three X-ray powderdiffraction peaks at 2θ angles selected from 9.4°, 11.1°, 16.5°, 18.3°and 18.8° and single crystalline Form C is characterized by at leastthree X-ray diffraction peaks at 2θ angles selected from 3.7°, 11.2′,12.1° and 18.6°; (b) heating the slurry to a temperature less than orequal to about 70° C. to form a second slurry or a solution; (c) addingwater to the second slurry or the solution and cooling the second slurryor the solution, thereby forming solid particles of crystalline Form Aof the compound of Structural Formula I; and (d) isolating the solidparticles of crystalline Form A, thereby preparing a compositioncomprising particles of single crystalline Form A of the compound ofStructural Formula I.
 57. The method of claim 55 or 56 wherein the solidparticles have a unimodal particle size distribution characterized by ad(0.9) of 100 microns or less.
 58. The method of claim 55 or 56, whereinthe solid particles have a unimodal particle size distributioncharacterized by a d(0.9) of 70 microns or less,
 59. The method of anyone of claims 55-58, wherein single crystalline Form B, C or D of thecompound of Structural Formula I or a mixture comprising two or more ofthe single crystalline forms of the compound of Structural Formula I, ofthe compound of Structural Formula I is suspended in a mixture ofisopropanol and water in step (a).
 60. The method of any one of claims55-59, wherein the ratio of isopropanol to water by volume in themixture of isopropanol and water is from about 0.1 to about
 4. 61. Themethod of claim 60, wherein the ratio of isopropanol to water by volumein the mixture of isopropanol and water is about
 1. 62. The method ofany one of claims 55-61, wherein single crystalline Form B, C or D ofthe compound of Structural Formula I or a mixture comprising two or moreof single crystalline Form B, C or D of the compound of StructuralFormula I, is suspended in an amount of isopropanol or mixture ofisopropanol and water of from about 5 to about 10 parts by weight withrespect to single crystalline Form B, C or D of the compound ofStructural Formula I or a mixture comprising two or more of singlecrystalline Form B, C or D of the compound of Structural Formula I. 63.The method of claim 55-61, wherein single crystalline Form B, C or D ofthe compound of Structural Formula I or a mixture comprising two or moreof single crystalline Form B, C or D of the compound of StructuralFormula I, is suspended in an amount of isopropanol or mixture ofisopropanol and water of from about 6 to about 7 parts by weight withrespect to single crystalline Form B, C or D of the compound ofStructural Formula I or a mixture comprising two or more of singlecrystalline Form B, C or D of the compound of Structural Formula I. 64.The method of any one of claims 55-63, wherein the slurry is heated to atemperature less than or equal to about 50° C.
 65. The method of any oneof claims 55-63, wherein the slurry is heated to a temperature of fromabout 35° C. to about 70° C.
 66. The method of claim 65, wherein theslurry is heated to a temperature of from about 35° C. to about 50° C.67. The method of claim 66, wherein the slurry is heated to atemperature of from about 65° C. to about 70° C.
 68. The method of anyone of claims 55-67, wherein the second slurry or the solution is cooledto from about 0° C. to about 55° C.
 69. The method of claim 68, whereinthe second slurry or the solution is cooled to from about 0° C. to about5° C.
 70. The method of claim 68, wherein the second slurry or thesolution is cooled to from about 15° C. to about 20° C.
 71. The methodof claim 68, wherein the second slurry or the solution is cooled to fromabout 45° C. to about 50° C.
 72. The method of any one of claims 55-71,wherein water is added to the second slurry or the solution in an amountof from about 5 parts by weight to about 15 parts by weight with respectto single crystalline Form B, C or D of the compound of StructuralFormula I or a mixture comprising two or more of single crystalline FormB, C or D of the compound of Structural Formula I.
 73. The method ofclaim 72, wherein water is added to the second slurry or the solution itan amount from about 7 parts by weight to about 10 parts by weight withrespect to single crystalline Form B, C or D of the compound ofStructural Formula I or a mixture comprising two or more of singlecrystalline Form B, C or D of the compound of Structural Formula I. 74.The method of claim 73, wherein water is added to the second slurry orthe solution it an amount of about 10 parts by weight with respect tosingle crystalline Form B, C or D of the compound of Structural FormulaI or a mixture comprising two or more of single crystalline Form B, C orD of the compound of Structural Formula I.
 75. A method of preparing asingle crystalline form of a compound represented by Structural FormulaI:

wherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9°, 21.3° and 22.0°, the method comprising: heating singlecrystalline Form B, C or D of the compound of Structural Formula I or amixture comprising two or more of crystalline Form B, C or D of thecompound of Structural Formula I and inducing formation of solidparticles of crystalline Form A; or maturing single crystalline Form B,C or D or the compound of Structural Formula I, or a mixture comprisingtwo or more of crystalline Form B, C or D of the compound of StructuralFormula I in a solvent system and inducing formation of solid particlesof crystalline Form A; or drying single crystalline Form B, C or D ofthe compound of Structural Formula I, or a mixture comprising two ormore of crystalline Form B, C or D of the compound of Structural FormulaI, thereby forming solid particles of crystalline Form A; or anycombination of the foregoing; and isolating the solid particles ofcrystalline Form A, thereby preparing single crystalline Form A of thecompound of Structural Formula I.
 76. The method of claim 75,comprising: heating a mixture comprising two or more of crystalline FormB, C or D of the compound of Structural Formula I and inducing formationof solid particles of crystalline Form A; or maturing a mixturecomprising two or more of crystalline Form B, C or D of the compound ofStructural Formula I in a solvent system and inducing formation of solidparticles of crystalline Form A; or drying a mixture comprising two ormore of crystalline Form B, C or D of the compound of Structural FormulaI, thereby forming solid particles of crystalline Form A; or anycombination of the foregoing.
 77. The method of claim 75 or 76, whereinthe mixture comprises of two or more crystalline forms optionallyincludes Form A.
 78. The method of claim 77, wherein the mixturecomprises two or more crystalline forms selected from Form B, Form C orForm D.
 79. The method of claim 75, comprising: heating singlecrystalline Form B, C or D of the compound of Structural Formula I andinducing formation of solid particles of crystalline Form A; or maturingsingle crystalline Form B, C or D of the compound of Structural FormulaI in a solvent system and inducing formation of solid particles ofcrystalline Form A; or drying single crystalline Form B, C or D of thecompound of Structural Formula I, thereby forming solid particles ofcrystalline Form A; or any combination of the foregoing.
 80. The methodof claim 75 or 79, wherein the single crystalline form is Form B. 81.The method of claim 75 or 79, wherein the single crystalline form isForm C.
 82. The method of claim 75 or 79, wherein the single crystallineform is Form D.
 83. A method of preparing a single crystalline form of acompound represented by Structural Formula I:

wherein the single crystalline form is Form D and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from3.7°, 7.3°, 10.9°, 18.3° and 21.9°, the method comprising: dissolvingthe compound of Structural Formula I in a solvent system comprisingacetonitrile; inducing formation of solid particles of crystalline FormD of the compound of Structural Formula I; and isolating the solidparticles of crystalline Form D, thereby preparing single crystallineForm D of the compound of Structural Formula I.
 84. The method of claim83, wherein single crystalline Form D is in the form of a solvate. 85.The method of claim 84, wherein the solvate is an acetonitrile solvate.86. The method of any one of claims 83-84, wherein the solvent systemcomprises greater than 20% by volume acetonitrile.
 87. The method ofclaim 86, wherein the solvent system comprises greater than or about 40%by volume acetonitrile.
 88. The method of claim 87, wherein the solventsystem comprises greater than or about 95% by volume acetonitrile. 89.The method of any one of claims 83-88, wherein inducing formation ofsolid particles of crystalline Form D comprises cooling the solutionformed by dissolving the compound of Structural Formula I in a solventsystem comprising acetonitrile.
 90. A method of preparing a compound ofStructural Formula I, comprising: combining a trialkylamine,2-methyltetrahydrofuran, a compound of Structural Formula II:

and a compound of Structural Formula III:

to form a reaction mixture; cooling the reaction mixture to from about−80° C. to about 0° C.; treating the reaction mixture withpropylphosphonic anhydride to provide a mixture comprising the compoundof Structural Formula I; and isolating the compound of StructuralFormula I from the mixture.
 91. The method of claim 90, wherein thetrialkylamine is diisopropylethylamine.
 92. The method of claim 90 or91, comprising cooling the reaction mixture to from about −50° C. toabout −15° C.
 93. The method of claim 92, comprising cooling thereaction mixture to from about −25° C. to about −20° C.
 94. The methodof any one of claims 90-93, wherein isolating the compound of StructuralFormula I from the mixture comprises adding an aqueous quench solutionto the mixture comprising the compound of Structural Formula I andperforming an extractive work-up of the resulting quenched reactionmixture.
 95. The method of claim 94, wherein the aqueous quench solutionis water and sue extractive work-up comprises washing the quenchedreaction mixture with an aqueous solution of sodium chloride.
 96. Amethod of preparing a single crystalline form of a compound representedby Structural Formula I:

wherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9°, 21.3° and 22.0°, the method comprising: (a) combining atrialkylamine, 2-methyltetrahydrofuran, a compound of Structural FormulaII:

and a compound of Structural Formula III:

to form a reaction mixture; (b) cooling the reaction mixture to fromabout −80° C. to about 0° C.; (c) treating the reaction mixture withpropylphosphonic anhydride to provide a mixture comprising the compoundof Structural Formula I; (d) isolating the compound of StructuralFormula I from the mixture; (e) dissolving the isolated compound ofStructural Formula I in a solvent system comprising acetonitrile; (f)inducing formation of solid particles of crystalline Form D of thecompound of Structural Formula I and isolating the solid particles ofcrystalline Form D to obtain single crystalline Form D of the compoundof Structural Formula I, or single crystalline Form B or C of thecompound of Structural Formula I or a mixture comprising two or more ofsingle crystalline Form B, C or D of the compound of Structural FormulaI, wherein single crystalline Form D is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 3.7°, 7.3°,10.9°, 18.3° and 21.9°; (g) heating single crystalline Form D, or singlecrystalline Form B or C of the compound of Structural Formula I or amixture comprising two or more of single crystalline Form B, C or D ofthe compound of Structural Formula I, and inducing formation of solidparticles of crystalline Form A; or maturing single crystalline Form D,or single crystalline Form B or C of the compound of Structural FormulaI or a mixture comprising two or more of crystalline Form B, C or D ofthe compound of Structural Formula I, in a solvent system and inducingformation of solid particles of crystalline Form A; or drying singlecrystalline Form D, or single crystalline Form B or C of the compound ofStructural Formula I or a mixture comprising two or more of crystallineForm B, C or D of the compound of Structural Formula I, thereby formingsolid particles of crystalline Form A; or any combination of theforegoing; and (h) isolating the particles of crystalline Form A,thereby preparing single crystalline Form A of the compound ofStructural Formula I.
 97. A method of preparing a single crystallineform of a compound represented by Structural Formula I:

wherein the single crystalline form is Form A and is characterized by atleast three X-ray powder diffraction peaks at 2θ angles selected from4.4°, 19.9°, 21.3° and 22.0°, the method comprising: (a) combining atrialkylamine, 2-methyltetrahydrofuran, a compound of Structural FormulaII:

and a compound of Structural Formula III:

to form a reaction mixture; (b) cooling the reaction mixture to fromabout −80° C. to about 0° C.; (c) treating the reaction mixture withpropylphosphonic anhydride to provide a mixture comprising the compoundof Structural Formula I; (d) isolating the compound of StructuralFormula I from the mixture; (e) dissolving the isolated compound ofStructural Formula I in a solvent system comprising acetonitrile;inducing formation of solid particles of crystalline Form D of thecompound of Structural Formula I and isolating the solid particles ofcrystalline Form D, or solid particles of single crystalline Form B or Cof the compound of Structural Formula I or a mixture comprising two ormore of single crystalline Form B, C or D of the compound of StructuralFormula I, to obtain single crystalline Form D of the compound ofStructural Formula I, or single crystalline Form B or C of the compoundof Structural Formula I or a mixture comprising two or more ofcrystalline Form B, C or D of the compound of Structural Formula I,wherein single crystalline Form D is characterized by at least threeX-ray powder diffraction peaks at 2θ angles selected from 3.7°, 7.3°,10.9°, 18.3° and 21.9°; (g) suspending single crystalline Form D, orsingle crystalline Form B or C of the compound of Structural Formula Ior a mixture comprising two or more of single crystalline Form B, C or Dof the compound of Structural Formula I in isopropanol or a mixture ofisopropanol and water to form a slurry; (h) heating the slurry to atemperature less than or equal to about 70° C. to form a second slurryor a solution; (i) cooling the second slurry or the solution and addingwater to the second slurry or the solution, thereby forming solidparticles of crystalline Form A of the compound of Structural Formula I;and (j) isolating the solid particles of crystalline Form A, therebypreparing single crystalline Form A of the compound of StructuralFormula I.